公开(公告)号：US20240309328A1

公开(公告)日：2024-09-19

申请号：US18270368

申请日：2022-01-04

Applicant: UNIVERSITY OF CONNECTICUT

Inventor： Cato T. Laurencin ,  Paulos Mengsteab ,  Takayoshi Otsuka

IPC: C12N5/077 ,  A61K38/00 ,  C07K14/50

CPC classification number: C12N5/0658 ,  C07K14/50 ,  C12N5/0655 ,  A61K38/00 ,  C12N2501/119 ,  C12N2506/1323 ,  C12N2506/1384

Abstract: Methods for enhancing myogenic and/or chondrogenic lineage commitment of stem cells, inhibiting adipogenic differentiation of stem cells, and for promoting tissue regeneration using fibroblast growth factor 8 (FGF8).

公开(公告)号：US20240240154A1

公开(公告)日：2024-07-18

申请号：US18473619

申请日：2023-09-25

Applicant: Children's Hospital Medical Center

Inventor： James M. Wells ,  Jorge Orlando Munera

IPC: C12N5/071

CPC classification number: C12N5/0679 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2506/02

Abstract: Disclosed herein are methods for the in vitro differentiation of a precursor cell into definitive endoderm, which may further be differentiated into a human colonic organoid (HCO), via modulation of signaling pathways. Further disclosed are HCOs and methods of using HCOs, which may be used, for example, for the HCOs may be used to determine the efficacy and/or toxicity of a potential therapeutic agent for a disease selected from colitis, colon cancer, polyposis syndromes, and/or irritable bowel syndrome.

公开(公告)号：US11981929B2

公开(公告)日：2024-05-14

申请号：US16950244

申请日：2020-11-17

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/40 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/22

Abstract: The present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells of a mature phenotype. The resulting pancreatic endocrine cells express single hormonal insulin, PDX1, NKX6.1, and MAFA. In one or more differentiation stages, culturing may be carried out in a culture vessel at the air-liquid interface.

公开(公告)号：US20240117304A1

公开(公告)日：2024-04-11

申请号：US18539589

申请日：2023-12-14

Applicant: KYOTO UNIVERSITY ,  SUMITOMO PHARMA CO., LTD.

Inventor： Jun TAKAHASHI ,  Daisuke DOI ,  Kenji YOSHIDA ,  Atsushi KUWAHARA ,  Masayo TAKAHASHI

IPC: C12N5/0793 ,  A61K35/30 ,  C12N5/074

CPC classification number: C12N5/0619 ,  A61K35/30 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/119 ,  C12N2501/13 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/90

Abstract: A cell population comprising Corin- and/or Lrtm1-positive cells was produced by the following steps (1) and (2), from which Corin positive and/or Lrtm1 positive cells are collected using a substance that binds to Corin and/or a substance that binds to Lrtm1, and dopaminergie neuron progenitor cells are produced by performing suspension culture of the Corin positive and/or Lrtm1 positive cells in a culture solution containing one or more nutritional factors:



(1) a step of performing adhesion culture of pluripotent stem cells in a medium for maintaining undifferentiated state containing a Sonic hedgehog (SHH) signal stimulant, and an undifferentiated state-maintaining factor in the absence of feeder cells but in the presence of an extracellular matrix, and
(2) a step of culturing the cell population obtained in the step (1) in a culture solution containing one or more differentiation-inducing factors.

公开(公告)号：US20240084259A1

公开(公告)日：2024-03-14

申请号：US17767822

申请日：2019-11-13

Applicant: KOREA UNIVERSITY RESEARCH AND BUSINESS FOUNDATION

Inventor： Do-Sun LIM ,  Seung Cheol CHOI ,  Hyung Joon JOO ,  Jong-Ho KIM ,  Chi-Yeon PARK ,  Yongdoo PARK

IPC: C12N5/077 ,  C12N5/00 ,  G01N33/50 ,  G01N33/68

CPC classification number: C12N5/0657 ,  C12N5/0018 ,  G01N33/5061 ,  G01N33/6887 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/119 ,  C12N2503/02 ,  G01N2333/805

Abstract: The present invention relates to stem cell-derived mature cardiomyocytes and a cardiovascular disease model using same and, more specifically, to differentiation into mature ventricular cardiomyocytes by culturing stem cells in a medium containing FGF4 and ascorbic acid, and use of the differentiated mature ventricular cardiomyocytes as a cardiovascular disease cell model. The mature ventricular cardiomyocytes, obtained by culturing stem cells in a medium containing FGF4 and ascorbic acid and inducing the differentiation thereof, and cardiovascular disease cell model using same according to the present invention are very useful for screening for cardiovascular disease therapeutic agents and evaluation of the toxicity of new drugs.

公开(公告)号：US20240052318A1

公开(公告)日：2024-02-15

申请号：US18354201

申请日：2023-07-18

Applicant: Lung Biotechnology PBC

Inventor： Adrian COOPER ,  Andre DHARMAWAN ,  Elena FEDERZONI ,  Kurt WONG

IPC: C12N5/071

CPC classification number: C12N5/0688 ,  C12N2501/117 ,  C12N2501/119 ,  C12N2501/39 ,  C12N2501/999 ,  C12N2501/01

Abstract: The present disclosure relates to methods of differentiating pluripotent stem cells or lung progenitor cells into alveolar type 2 (AT2) cells. The present disclosure also relates to AT2 cells made by such methods, organoids containing such AT2 cells and methods of using the same. The present disclosure also relates to differentiation media for use in the same.

公开(公告)号：US20240043812A1

公开(公告)日：2024-02-08

申请号：US18359916

申请日：2023-07-27

Applicant: Centre for Immunology & Infection Limited

Inventor： Chi Wai Michael CHAN ,  Pui Yan HUI ,  Hiu Ha CHING

IPC: C12N5/071

CPC classification number: C12N5/0688 ,  C12N2513/00 ,  C12N2501/155 ,  C12N2501/415 ,  C12N2501/119 ,  C12N2501/117 ,  C12N2509/00 ,  C12N2509/10 ,  C12N2503/00 ,  C12N2533/54

Abstract: The present invention relates to a non-invasive method for generating human three-dimensional nasopharyngeal organoids, including non-invasively collecting nasopharyngeal swab samples; culturing the samples in a matrix with niche factors; and obtaining the three-dimensional human nasopharyngeal organoids after culturing the samples in the matrix for 14-21 days. The three-dimensional human nasopharyngeal organoids are then dissociated into a single cell suspension for the creation of human two-dimensional nasopharyngeal organoids. The present nasopharyngeal organoids and their preparation method enable the rapid expansion of stem cells to form organoids within a short period of time. By combining organoid passaging techniques, a sufficient number of organoids can be obtained within a limited time for research and experimental operations. These organoid models provide new and excellent tools to study the transmission, tropism, and innate host responses of emerging viruses such as influenza virus and coronavirus, aiding in the evaluation of the pathophysiological characteristics of the viruses.

公开(公告)号：US20230384291A1

公开(公告)日：2023-11-30

申请号：US18029026

申请日：2021-09-28

Applicant: National University Corporation Tokyo Medical and Dental University ,  Takeda Pharmaceutical Company Limited

Inventor： Takanori TAKEBE ,  Norikazu SAIKI

IPC: G01N33/50 ,  C12N5/071 ,  C12M3/00 ,  C12M1/12

CPC classification number: G01N33/5082 ,  C12N5/0697 ,  C12M21/08 ,  C12M25/04 ,  C12N2501/119 ,  C12N2501/16 ,  C12N2513/00

Abstract: The present invention provides means for producing a parathyroid gland organoid. A culture method of the present invention includes a cell population containing a parathyroid gland cell in a ratio of 50% or more, and a mesenchymal cell in a ratio of 10% or more; and a step of culturing the cell population.

公开(公告)号：US20230374448A1

公开(公告)日：2023-11-23

申请号：US17913535

申请日：2021-04-06

Applicant: RXCELL INC.

Inventor： Xianmin ZENG ,  Mahendra RAO

IPC: C12N5/0793 ,  C12N15/90 ,  C12N5/00 ,  C07K14/54 ,  C07K14/52

CPC classification number: C12N5/0619 ,  C12N5/062 ,  C12N15/907 ,  C12N5/0006 ,  C07K14/5428 ,  C07K14/521 ,  C12N2506/45 ,  C12N2509/00 ,  C12N2501/105 ,  C12N2501/41 ,  C12N2501/119

Abstract: Hypoimmunogenic cell lines and methods and compositions for their production are provided.

公开(公告)号：US20230365939A1

公开(公告)日：2023-11-16

申请号：US18027900

申请日：2021-09-22

Applicant: CHILDREN'S HOSPITAL MEDICAL CENTER

Inventor： Vered Shacham-Silverberg ,  James Macormack Wells

IPC: C12N5/071

CPC classification number: C12N5/0679 ,  C12N2533/90 ,  C12N2501/119 ,  C12N2501/155

Abstract: Disclosed herein are esophageal raft culture compositions that more closely resemble native organ structures. These esophageal raft cultures may also be innervated by combination with enteric neural crest cells. These raft culture compositions are advantageous for purposes such as studying organellar function, development, and organization. Also disclosed herein are methods of producing said esophageal raft culture compositions.