公开(公告)号：US20200370020A1

公开(公告)日：2020-11-26

申请号：US16988145

申请日：2020-08-07

申请人： PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY ,  FUJIFILM Corporation

发明人： Tamihide MATSUNAGA ,  Takahiro Iwao ,  Tomoki Kabeya ,  Shinji Mima ,  Toshihide Miyashita

IPC分类号： C12N5/071

摘要： An object of the present invention is to provide a new means capable of easily and efficiently preparing cells showing a function more similar to the function of intestinal epithelial cells of a living body. According to the present invention, pluripotent stem cells are induced to differentiate into intestinal stem cell-like cells by a method for inducing differentiation of pluripotent stem cells into intestinal epithelial cells, the method including a step (1) of differentiating pluripotent stem, cells into intestinal stem cell-like cells and a step (2) of differentiating the intestinal stem cell-like cells obtained in the step (1) into intestinal epithelial cell-like cells by using an MEK1 inhibitor, a DNA methylation inhibitor, a TGFβ receptor inhibitor, EGF, and a cAMP activator in combination.

公开(公告)号：US20220125854A1

公开(公告)日：2022-04-28

申请号：US17436205

申请日：2020-02-12

申请人： PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY

发明人： Tamihide MATSUNAGA ,  Tadahiro HASHITA ,  Hiromasa AOKI

IPC分类号： A61K35/44 ,  C12N5/071

摘要： It is a subject to provide a means of preparing highly pure vascular endothelial progenitor cells in a simple and low-cost manner. It is also a subject to provide a method for efficiently proliferating vascular endothelial progenitor cells. High-purity vascular endothelial progenitor cells are prepared by the process of differentiating pluripotent stem cells into vascular endothelial progenitor cells and purifying the vascular endothelial progenitor cells using a difference in adhesion ability between the vascular endothelial progenitor cells constituting the cell population obtained in the process and other cells. On the other hand, vascular endothelial progenitor cells are cultured and expanded in the presence of a ROCK inhibitor, a GSK-3β inhibitor, and a TGF-β receptor inhibitor in addition to basic fibroblast growth factor and epidermal growth factor.

公开(公告)号：US20220033780A1

公开(公告)日：2022-02-03

申请号：US17290446

申请日：2019-10-31

申请人： PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY

发明人： Tamihide MATSUNAGA ,  Takahiro IWAO ,  Daichi ONOZATO ,  Isamu OGAWA

IPC分类号： C12N5/071 ,  G01N33/50 ,  A61L27/38

摘要： An object is to prepare an intestinal organoid having a characteristic close to the small intestine of a living body, from a pluripotent stem cell. An intestinal organoid is prepared from a pluripotent stem cell, by the following steps of: (1) differentiating the pluripotent stem cell into an endoderm-like cell; (2) differentiating the endoderm-like cell obtained in step (1) into an intestinal stem cell-like cell; (3) culturing the intestinal stem cell-like cell obtained in step (2) in the presence of an epidermal growth factor, a fibroblast growth factor, a TGF β receptor inhibitor, a GSK-3 β inhibitor, and a ROCK inhibitor; (4) culturing the cell obtained in step (3) to form a spheroid; and (5) differentiating the spheroid formed in step (4) to form an intestinal organoid, wherein the differentiation includes culturing in the presence of an epidermal growth factor, a BMP inhibitor, and a Wnt signal activator. Also, a plane culture system is prepared by subjecting the cells constituting the intestinal organoid formed in step (5) to plane culture in the presence of an epidermal growth factor and a TGF β receptor inhibitor. A highly functional evaluation system having the villi structure is constructed by using air-liquid interface culture in the plane culture.

公开(公告)号：US20230084457A1

公开(公告)日：2023-03-16

申请号：US17798206

申请日：2021-02-09

申请人： PUBLIC UNIVERSITY CORPORATION NAGOYA CITY UNIVERSITY

发明人： Tamihide MATSUNAGA ,  Tadahiro HASHITA ,  Hiromasa AOKI ,  Misaki YAMASHITA

IPC分类号： G01N33/50 ,  C12N5/071

摘要： Provided is a technology allowing for stable supply of brain microvascular endothelium-like cells. This coating agent for inducing differentiation of pluripotent stem cells into brain microvascular endothelium-like cells contains at least one component of a Laminin-221 fragment or an N-terminal Vitronectin.