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公开(公告)号：US20060258804A1
公开(公告)日：2006-11-16
申请号：US11431996
申请日：2006-05-11
IPC分类号： C08L53/00
CPC分类号： C08F283/00 , C08F271/00 , C08F283/04 , C08G73/0206 , C08G83/003 , C12N15/87 , Y10T428/31848
摘要： Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.
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公开(公告)号：US20050079149A1
公开(公告)日：2005-04-14
申请号：US10806025
申请日：2004-03-22
IPC分类号： A61K31/74 , A61K31/785 , C08F8/32 , C08F285/00 , C08G63/48 , C08G63/91 , C12Q20060101
CPC分类号： C08F283/00 , C08F271/00 , C08F283/04 , C08G73/0206 , C08G83/003 , C12N15/87 , Y10T428/31848
摘要： Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.
摘要翻译： 使用低分子量聚乙烯亚胺（BPEI-L）作为核心并用于基因递送合成超支化树突（HD）聚合物。 所获得的聚合物在低氮磷酸盐（N / P）比低的情况下显示低毒性和有效的基因递送。 使用乙烯亚胺部分连续连接到PEI芯上，该聚合物具有比在芯PEI中更低的线性至支化结构的相对比例。 更广泛的分支使得聚合物能够将质粒DNA冷凝成尺寸小于或等于约100nm的纳米结构复合物。 复合物在血清存在下转染细胞是稳定和有效的。 使用在活鼠中进行的荧光素酶报告基因的体内基因表达的生物发光成像显示在肝脏和下颌下淋巴结中的基因表达。
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公开(公告)号：US07153905B2
公开(公告)日：2006-12-26
申请号：US10806025
申请日：2004-03-22
IPC分类号： C08F8/30
CPC分类号： C08F283/00 , C08F271/00 , C08F283/04 , C08G73/0206 , C08G83/003 , C12N15/87 , Y10T428/31848
摘要： Hyperbranched dendron (HD) polymers are synthesized using low molecular weight polyethyleneimine (BPEI-L) as a core and used for gene delivery. The obtained polymers display low toxicity and efficient gene delivery at low nitrogen-to-phosphate (N/P) ratios. Using successive attachment of ethyleneimine moieties to a PEI core, the polymer has a lower relative ratio of linear-to-branched structures than in the core PEI. The more extensive branching enables the polymer to condense plasmid DNA into nanostructure complexes with a size of less than or equal to about 100 nm. The complexes are stable and efficient in transfecting cells in the presence of serum. Bioluminescent imaging of in vivo gene expression using a luciferase reporter gene performed in live mice showed gene expression in the liver and in submandibular lymph nodes.
摘要翻译： 使用低分子量聚乙烯亚胺（BPEI-L）作为核心并用于基因递送合成超支化树突（HD）聚合物。 所获得的聚合物在低氮磷酸盐（N / P）比低的情况下显示低毒性和有效的基因递送。 使用乙烯亚胺部分连续连接到PEI芯上，该聚合物具有比在芯PEI中更低的线性至支化结构的相对比例。 更广泛的分支使得聚合物能够将质粒DNA冷凝成尺寸小于或等于约100nm的纳米结构复合物。 复合物在血清存在下转染细胞是稳定和有效的。 使用在活鼠中进行的荧光素酶报告基因的体内基因表达的生物发光成像显示在肝脏和下颌下淋巴结中的基因表达。
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