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公开(公告)号:US20170315087A1
公开(公告)日:2017-11-02
申请号:US15279548
申请日:2016-09-29
Inventor: MARI ONODERA , HIDENOBU YAKU
IPC: G01N27/416 , G01N27/447 , G01N27/06
CPC classification number: G01N27/4163 , G01N27/06 , G01N27/44704 , G01N27/44747 , G01N27/44791
Abstract: The present invention provides a method for determining whether or not a capillary filled with an electrophoretic medium is suitably used for electrophoresis before electrophoresis is performed using the analytes. The method comprises (a) applying an alternating-current voltage between a first electrode which is in contact with a first electrolyte solution in which one end of the capillary is immersed and a second electrode which is in contact with a second electrolyte solution in which the other end of the capillary is immersed to measure an electric conductivity of the electrophoresis medium with which an inside of the capillary is filled; and (b) determining that the capillary filled with the electrophoresis medium fails to be used suitably for the electrophoresis, when the electric conductivity is more than 4.2 mS/cm.
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2.
公开(公告)号:US20170275698A1
公开(公告)日:2017-09-28
申请号:US15257782
申请日:2016-09-06
Inventor: HIDENOBU YAKU
IPC: C12Q1/68
CPC classification number: C12Q1/6886 , C12Q1/686 , C12Q2521/113 , C12Q2521/327 , C12Q2565/1015
Abstract: The present invention provides a method for determining whether or not an aqueous solution contains two or more cancer cells. The present method is characterized by the following three matters. First, the PCR solution contains the TS primer at a concentration of not less than 0.1 μM and not more than 1 μM in the present invention. Second, the PCR solution contains an ACX reverse primer. Third, the PCR solution contains the ACX reverse primer at a concentration of not less than 0.02 μM and not more than 0.06 μM in the present invention. In the present method, it is determined that an aqueous solution contains cancer cells even if the aqueous solution contains only two cancer cells.
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