公开(公告)号：US20210040531A1

公开(公告)日：2021-02-11

申请号：US17069905

申请日：2020-10-14

Applicant: Panasonic Intellectual Property Management Co., Ltd.

Inventor： TAROU ISHIDOU ,  YOSIFUMI KARIATUMARI

IPC: C12Q1/04 ,  C12M1/34

Abstract: The present disclosure provides a simple and secure apparatus and a simple and secure method for selectively detecting a tomato pathogenic fungus. The tomato pathogenic fungus detecting apparatus according to the present disclosure is characterized by including an artificial cell wall, a test sample solution inlet provided above the artificial cell wall, and a culture solution storage part provided under the artificial cell wall, wherein a test sample solution contains a 50 mM to 70 mM buffer solution of a citrate salt in the test sample solution inlet, and the test sample solution has a pH of 5 to 5.5.

公开(公告)号：US20210024874A1

公开(公告)日：2021-01-28

申请号：US17069911

申请日：2020-10-14

Applicant: Panasonic Intellectual Property Management Co., Ltd.

Inventor： TAROU ISHIDOU ,  YOSIFUMI KARIATUMARI

IPC: C12M1/34 ,  C12Q1/04

Abstract: The present disclosure provides a simple and secure apparatus and a simple and secure method for selectively detecting a tomato pathogenic fungus. The tomato pathogenic fungus detecting apparatus according to the present disclosure is characterized by including an artificial cell wall, a test sample solution inlet provided above the artificial cell wall, and a culture solution storage part provided under the artificial cell wall, wherein a culture solution contains a 15 mM to 30 mM buffer solution of a citrate salt in the culture solution storage part, and the culture solution has a pH of 5 to 5.5.

公开(公告)号：US20190390244A1

公开(公告)日：2019-12-26

申请号：US16561028

申请日：2019-09-05

Applicant: Panasonic Intellectual Property Management Co., Ltd.

Inventor： YUKI KUSHIDA ,  YOSIFUMI KARIATUMARI

IPC: C12Q1/04 ,  G01N21/31

Abstract: The present invention provides a method for determining whether or not a sample contains at least one of Cercospora fungus and Pseudocercospora fungus. In the method, first, the sample is added an aqueous solution containing N,N,N′,N′-Tetramethyl-1,4-phenylenediamine and 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazolium to provide a mixture. The aqueous solution has a pH of not less than 5.1 and not more than 6.5. Then, the mixture is irradiated with light having a wavelength of 400 to 500 nanometers to measure a first absorbance Ay of the mixture, and the mixture is irradiated with light having a wavelength of 500 to 800 nanometers to measure a second absorbance Ax of the mixture. If the value of Ay/Ax is not less than 1.5 or the mixture is orangey, it is determined that the sample contains at least one of Cercospora fungus and Pseudocercospora fungus.