公开(公告)号：US06589797B2

公开(公告)日：2003-07-08

申请号：US09866169

申请日：2001-05-25

申请人： Paschalis Alexandridis ,  Sriram Neelamegham

发明人： Paschalis Alexandridis ,  Sriram Neelamegham

IPC分类号： G01N100

CPC分类号： G01N1/34 ,  G01N1/405 ,  G01N2001/005 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： The present method describes a method for recovery of aqueous samples or components thereof from polymer packaging materials, wherein the spilled aqueous sample has been absorbed by the packaging material to form a liquid swollen absorbent gel. The method comprises the steps of contacting the liquid swollen absorbent gel with a recover fluid, wherein the recovery fluid has a higher osmotic pressure then the aqueous sample, disrupting the gel and removing the recovery mixture for detection and analysis of analyte.

摘要翻译： 本方法描述了从聚合物包装材料中回收水性样品或其组分的方法，其中溢出的含水样品已被包装材料吸收以形成液体溶胀的吸收凝胶。 该方法包括以下步骤：将液体溶胀的吸收凝胶与回收流体接触，其中回收流体具有较高的渗透压，然后是水性样品，破坏凝胶并除去回收混合物以用于检测和分析分析物。

公开(公告)号：US07008797B2

公开(公告)日：2006-03-07

申请号：US10615056

申请日：2003-07-08

申请人： Paschalis Alexandridis ,  Sriram Neelamegham

发明人： Paschalis Alexandridis ,  Sriram Neelamegham

IPC分类号： G01N1/00

CPC分类号： G01N1/34 ,  G01N1/405 ,  G01N2001/005 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255

摘要： The present invention describes a method for recovery of aqueous samples or components thereof from polymer packaging materials, wherein the spilled aqueous sample has been absorbed by the packaging material to form a liquid swollen absorbent gel. The method comprises the steps of contacting the liquid swollen absorbent gel with a recovery fluid, wherein the recovery fluid has a higher osmotic pressure than the aqueous sample.

公开(公告)号：US08278072B1

公开(公告)日：2012-10-02

申请号：US12274142

申请日：2008-11-19

申请人： Khushi L. Matta ,  Edaya V. Chandrasekaran ,  Sriram Neelamegham ,  Jun Xue

发明人： Khushi L. Matta ,  Edaya V. Chandrasekaran ,  Sriram Neelamegham ,  Jun Xue

IPC分类号： C12P19/18

CPC分类号： C12P19/18 ,  C12P7/6436 ,  C12P19/44 ,  C12P21/005 ,  C12Y204/99004

摘要： A method for producing sialylated molecules based on reverse sialylation that catalytically transfers the sialic acid moiety of sialylated donors to nucleotide monophosphates or transfer sialic acid moieties from sialylated donors to acceptor glycoproteins or glycolipids.

摘要翻译： 基于反向唾液酸化制备唾液酸化分子的方法，其将唾液酸化供体的唾液酸部分催化转移到核苷酸单磷酸酯或将唾液酸部分从唾液酸化供体转移到受体糖蛋白或糖脂。

公开(公告)号：US20130078661A1

公开(公告)日：2013-03-28

申请号：US13701930

申请日：2011-06-06

申请人： Sriram Neelamegham ,  Kannayakanahalli Dayananda

发明人： Sriram Neelamegham ,  Kannayakanahalli Dayananda

IPC分类号： C07K14/755 ,  C12Q1/37

CPC分类号： C07K14/755 ,  C12Q1/37 ,  G01N2333/96486

摘要： Provided are compositions and methods for Fluorescence/Forster Resonance Energy Transfer (FRET) analysis of cleavage of Von Willebrand Factor (VWF) polypeptides by the metalloprotease ADAMTS13. The polypeptides contain: i) a first fluorescent protein ii) a VWF amino acid sequence and iii) a second fluorescent protein. The VWF amino acid can contain any of a variety of amino acid substitutions. The method involves contacting a sample that contains ADAMTS13 and using FRET based measurements to determine ADAMTS13 cleavage of the recombinant polypeptide.

摘要翻译： 提供了通过金属蛋白酶ADAMTS13切割Von Willebrand因子（VWF）多肽的荧光/福斯特共振能量转移（FRET）分析的组合物和方法。 多肽包含：i）第一荧光蛋白ii）VWF氨基酸序列和iii）第二荧光蛋白。 VWF氨基酸可以含有任何多种氨基酸取代。 该方法包括接触包含ADAMTS13的样品并使用基于FRET的测量来确定重组多肽的ADAMTS13切割。