公开(公告)号：US08968746B2

公开(公告)日：2015-03-03

申请号：US13203653

申请日：2011-07-25

申请人： Patrick Baumhof ,  Söhnke Voss ,  Thomas Kramps ,  Karl-Josef Kallen

发明人： Patrick Baumhof ,  Söhnke Voss ,  Thomas Kramps ,  Karl-Josef Kallen

IPC分类号： A61K39/385 ,  A61K45/00 ,  A61K47/00 ,  A61K48/00 ,  A61K39/39 ,  C12N15/87 ,  A61K39/00

CPC分类号： A61K48/0041 ,  A61K39/00 ,  A61K39/385 ,  A61K39/39 ,  A61K47/645 ,  A61K2039/51 ,  A61K2039/55516 ,  A61K2039/55561 ,  C12N15/87 ,  Y02A50/412 ,  Y02A50/472

摘要： The present invention is directed to a polymeric carrier cargo complex, comprising as a cargo at least one nucleic acid (molecule) and disulfide-crosslinked cationic components as a (preferably non-toxic and non-immunogenic) polymeric carrier. The inventive polymeric carrier cargo complex allows for both efficient transfection of nucleic acids into cells in vivo and in vitro and/or for induction of an (innate and/or adaptive) immune response, preferably dependent on the nucleic acid to be transported as a cargo. The present invention also provides, pharmaceutical compositions, particularly vaccines and adjuvants, comprising the inventive polymeric carrier cargo complex and optionally an antigen, as well as the use of such the inventive polymeric carrier cargo complex and optionally an antigen for transfecting a cell, a tissue or an organism, for (gene-)therapeutic purposes as disclosed herein, and/or as an immunostimulating agent or adjuvant, e.g. for eliciting an immune response for the treatment or prophylaxis of diseases as mentioned above. Finally, the invention relates to kits containing the inventive polymeric carrier cargo complex and/or the inventive pharmaceutical composition, adjuvant or vaccine in one or more parts of the kit.

摘要翻译： 本发明涉及一种聚合物载体货物复合物，其包含至少一种核酸（分子）和作为（优选无毒的和非免疫原性的）聚合物载体的二硫键交联的阳离子组分。 本发明的聚合物载体货物复合物允许在体内和体外有效地将核酸转染到细胞中和/或诱导（先天和/或自适应）免疫应答，优选依赖于作为货物运输的核酸 。 本发明还提供药物组合物，特别是疫苗和佐剂，其包含本发明的聚合物载体货物复合物和任选的抗原，以及使用本发明的本发明的聚合物载体货物复合物和任选的抗原用于转染细胞，组织 或用于本文公开的（基因 - ）治疗目的的生物体，和/或作为免疫刺激剂或佐剂，例如， 用于引发用于治疗或预防上述疾病的免疫应答。 最后，本发明涉及在试剂盒的一个或多个部分中含有本发明的聚合物载体货物复合物和/或本发明的药物组合物，佐剂或疫苗的试剂盒。

公开(公告)号：US20130142818A1

公开(公告)日：2013-06-06

申请号：US13203653

申请日：2011-07-25

申请人： Patrick Baumhof ,  Söhnke Voss ,  Thomas Kramps ,  Karl-Josef Kallen

发明人： Patrick Baumhof ,  Söhnke Voss ,  Thomas Kramps ,  Karl-Josef Kallen

IPC分类号： A61K48/00

CPC分类号： A61K48/0041 ,  A61K39/00 ,  A61K39/385 ,  A61K39/39 ,  A61K47/645 ,  A61K2039/51 ,  A61K2039/55516 ,  A61K2039/55561 ,  C12N15/87 ,  Y02A50/412 ,  Y02A50/472

摘要： The present invention is directed to a polymeric carrier cargo complex, comprising as a cargo at least one nucleic acid (molecule) and disulfide-crosslinked cationic components as a (preferably non-toxic and non-immunogenic) polymeric carrier. The inventive polymeric carrier cargo complex allows for both efficient transfection of nucleic acids into cells in vivo and in vitro and/or for induction of an (innate and/or adaptive) immune response, preferably dependent on the nucleic acid to be transported as a cargo. The present invention also provides, pharmaceutical compositions, particularly vaccines and adjuvants, comprising the inventive polymeric carrier cargo complex and optionally an antigen, as well as the use of such the inventive polymeric carrier cargo complex and optionally an antigen for transfecting a cell, a tissue or an organism, for (gene-)therapeutic purposes as disclosed herein, and/or as an immunostimulating agent or adjuvant, e.g. for eliciting an immune response for the treatment or prophylaxis of diseases as mentioned above. Finally, the invention relates to kits containing the inventive polymeric carrier cargo complex and/or the inventive pharmaceutical composition, adjuvant or vaccine in one or more parts of the kit.

公开(公告)号：US09226959B2

公开(公告)日：2016-01-05

申请号：US12672442

申请日：2009-01-28

申请人： Thomas Kramps ,  Söhnke Voss ,  Jochen Probst ,  Ingmar Hoerr

发明人： Thomas Kramps ,  Söhnke Voss ,  Jochen Probst ,  Ingmar Hoerr

IPC分类号： C07H21/02 ,  A61K39/00 ,  A61K39/39 ,  A61P37/04 ,  C07H21/00 ,  C12N15/117

CPC分类号： A61K39/39 ,  A61K2039/53 ,  A61K2039/55561 ,  A61K2039/572 ,  C07H21/00 ,  C12N15/117 ,  C12N2310/17 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2320/31 ,  Y02A50/491

摘要： The present invention relates to nucleic acids of the general formula (I): (NuGlXmGnNv)a and derivatives thereof as an immunostimulating agent/adjuvant and to compositions containing same, optionally comprising an additional adjuvant. The present invention furthermore relates to a pharmaceutical composition or to a vaccine, each containing nucleic acids of formula (I) above and/or derivatives thereof as an immunostimulating agent, and optionally at least one additional pharmaceutically active component, e.g. an antigenic agent. The present invention relates likewise to the use of the pharmaceutical composition or of the vaccine for the treatment of cancer diseases, infectious diseases, allergies and autoimmune diseases etc. Likewise, the present invention includes the use of nucleic acids of the general formula (I): (NuGlXmGnNv)a and/or derivatives thereof for the preparation of a pharmaceutical composition for the treatment of such diseases.

摘要翻译： 本发明涉及通式（I）的核酸：（NuG1XmGnNv）a及其衍生物作为免疫刺激剂/佐剂和含有其的组合物，任选地包含另外的佐剂。 本发明还涉及药物组合物或疫苗，其各自含有上述式（I）核酸和/或其衍生物作为免疫刺激剂，以及任选的至少一种另外的药物活性成分，例如， 抗原剂。 本发明同样涉及药物组合物或疫苗用于治疗癌症疾病，感染性疾病，过敏症和自身免疫疾病等的用途。同样地，本发明包括通式（I）的核酸， ：（NuGlXmGnNv）a和/或其衍生物，用于制备用于治疗这些疾病的药物组合物。

公开(公告)号：US09572874B2

公开(公告)日：2017-02-21

申请号：US12994407

申请日：2009-09-30

申请人： Mariola Fotin-Mleczek ,  Söhnke Voss

发明人： Mariola Fotin-Mleczek ,  Söhnke Voss

IPC分类号： A61K48/00 ,  A61K47/06 ,  A61K39/00

CPC分类号： A61K39/39 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/55511 ,  Y02A50/491

摘要： The present invention relates to an immunostimulatory composition comprising a) an adjuvant component, comprising or consisting of at least one (m)RNA, complexed with a cationic or polycationic compound, and b) at least one free mRNA, encoding at least one therapeutically active protein, antigen, allergen and/or antibody, wherein the immunostimulatory composition is capable to elicit or enhance an innate and optionally an adaptive immune response in a mammal. The inventive immunostimulatory composition may be a pharmaceutical composition or a vaccine. The invention furthermore relates to a method of preparation of the inventive immunostimulatory composition. The invention also relates to the use of the inventive immunostimulatory composition or its components (for the preparation of a pharmaceutical composition or a vaccine) for the treatment of various diseases. Finally, the invention relates to kits containing the inventive immunostimulatory composition, its components and/or the pharmaceutical composition or vaccine.

摘要翻译： 本发明涉及免疫刺激组合物，其包含a）佐剂组分，其包含或与由阳离子或聚阳离子化合物络合的至少一种（m）RNA组成，和b）至少一种游离mRNA，其编码至少一种治疗活性 蛋白质，抗原，变应原和/或抗体，其中所述免疫刺激组合物能够引发或增强哺乳动物中的先天和任选的适应性免疫应答。 本发明的免疫刺激组合物可以是药物组合物或疫苗。 本发明还涉及本发明的免疫刺激组合物的制备方法。 本发明还涉及本发明的免疫刺激组合物或其组分（用于制备药物组合物或疫苗）用于治疗各种疾病的用途。 最后，本发明涉及包含本发明的免疫刺激组合物，其组分和/或药物组合物或疫苗的试剂盒。

公开(公告)号：US20110250225A1

公开(公告)日：2011-10-13

申请号：US12994407

申请日：2009-09-30

申请人： Mariola Fotin-Mleczek ,  Söhnke Voss

发明人： Mariola Fotin-Mleczek ,  Söhnke Voss

IPC分类号： A61K39/39 ,  A61P37/04 ,  A61P37/08 ,  A61P31/04 ,  A61P31/12 ,  A61P33/02 ,  A61P35/00 ,  A61P31/00

CPC分类号： A61K39/39 ,  A61K39/0011 ,  A61K2039/53 ,  A61K2039/55511 ,  Y02A50/491

摘要： The present invention relates to an immunostimulatory composition comprising a) an adjuvant component, comprising or consisting of at least one (m)RNA, complexed with a cationic or polycationic compound, and b) at least one free mRNA, encoding at least one therapeutically active protein, antigen, allergen and/or antibody, wherein the immunostimulatory composition is capable to elicit or enhance an innate and optionally an adaptive immune response in a mammal. The inventive immunostimulatory composition may be a pharmaceutical composition or a vaccine. The invention furthermore relates to a method of preparation of the inventive immunostimulatory composition. The invention also relates to the use of the inventive immunostimulatory composition or its components (for the preparation of a pharmaceutical composition or a vaccine) for the treatment of various diseases. Finally, the invention relates to kits containing the inventive immunostimulatory composition, its components and/or the pharmaceutical composition or vaccine.

摘要翻译： 本发明涉及免疫刺激组合物，其包含a）佐剂组分，其包含或与由阳离子或聚阳离子化合物络合的至少一种（m）RNA组成，和b）至少一种游离mRNA，其编码至少一种治疗活性 蛋白质，抗原，变应原和/或抗体，其中所述免疫刺激组合物能够引发或增强哺乳动物中的先天和任选的适应性免疫应答。 本发明的免疫刺激组合物可以是药物组合物或疫苗。 本发明还涉及本发明的免疫刺激组合物的制备方法。 本发明还涉及本发明的免疫刺激组合物或其组分（用于制备药物组合物或疫苗）用于治疗各种疾病的用途。 最后，本发明涉及包含本发明的免疫刺激组合物，其组分和/或药物组合物或疫苗的试剂盒。