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15 条记录 (耗时 0.029 秒)

    Structure and expression of an arabidopsis acetyl-coenzyme A carboxylase gene
    6.
    发明授权
    Structure and expression of an arabidopsis acetyl-coenzyme A carboxylase gene 失效
    拟南芥乙酰辅酶A羧化酶基因的结构和表达

    公开(公告)号：US5962767A

    公开(公告)日：1999-10-05

    申请号：US790519

    申请日：1997-01-29

    申请人： John B. Ohlrogge ,  Keith R. Roesler ,  Basil S. Shorrosh

    发明人： John B. Ohlrogge ,  Keith R. Roesler ,  Basil S. Shorrosh

    IPC分类号： C12N9/00 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10 ,  C12N5/04

    CPC分类号： C12N9/93 ,  C12N15/8247

    摘要： An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.

    摘要翻译： 拟南芥乙酰辅酶A羧化酶（ACCase）基因已被分离并测序。 10 kb基因编码251 kD胞质ACCase同工酶。 该基因的核酸序列如SEQ ID No.1所示，并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。 通过控制本发明的基因的表达，可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此，通过在正义或反义方向引入本发明基因的构建体，可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此，也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。

    Methods of increasing oil content of seeds
    7.
    发明授权
    Methods of increasing oil content of seeds 失效
    增加种子油含量的方法

    公开(公告)号：US5925805A

    公开(公告)日：1999-07-20

    申请号：US677010

    申请日：1996-07-01

    申请人： John B. Ohlrogge ,  Keith R. Roesler ,  Basil S. Shorrosh

    发明人： John B. Ohlrogge ,  Keith R. Roesler ,  Basil S. Shorrosh

    IPC分类号： C12N9/00 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8247 ,  C12N9/93

    摘要： An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.

    摘要翻译： 拟南芥乙酰辅酶A羧化酶（ACCase）基因已被分离并测序。 10-kb基因编码251-kd胞质ACCase同工酶。 该基因的核酸序列如SEQ ID No.1所示，并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。 通过控制本发明的基因的表达，可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此，通过在正义或反义方向引入本发明基因的构建体，可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。 因此，也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。

    Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof
    8.
    发明授权
    Plant cyclopropane fatty acid synthase genes, proteins, and uses thereof 失效
    植物环丙烷脂肪酸合成酶基因，蛋白质及其用途

    公开(公告)号：US07446188B2

    公开(公告)日：2008-11-04

    申请号：US10499246

    申请日：2002-12-20

    申请人： Xiaoming Bao ,  John B. Ohlrogge ,  Michael R. Pollard

    发明人： Xiaoming Bao ,  John B. Ohlrogge ,  Michael R. Pollard

    IPC分类号： C07H21/04 ,  A61K36/00

    CPC分类号： C12N9/1007 ,  C12N15/8247

    摘要： The present invention provides cyclopropane fatty acid synthase genes and proteins, and methods of their use. The present invention encompasses both native and recombinant wild-type forms of the synthase, as well as mutants and variant forms, some of which possess altered characteristics relative to the wild-type synthase. The present invention also provides methods of using cyclopropane fatty acid synthase genes and proteins, including in their expression in transgenic organisms and in the production of cyclopropane fatty acids in plant oils, and in particular seed oils.

    摘要翻译： 本发明提供了环丙烷脂肪酸合酶基因和蛋白质及其使用方法。 本发明包括合成酶的天然和重组野生型形式，以及突变体和变体形式，其中一些相对于野生型合酶具有改变的特征。 本发明还提供了使用环丙烷脂肪酸合酶基因和蛋白质的方法，包括在转基因生物体中的表达和在植物油中特别是种子油中的环丙烷脂肪酸的生产。

    Synthetic gene for acyl carrier protein
    10.
    发明授权
    Synthetic gene for acyl carrier protein 失效
    酰基载体蛋白的合成基因

    公开(公告)号：US4888282A

    公开(公告)日：1989-12-19

    申请号：US58054

    申请日：1987-06-04

    申请人： Phillips D. Beremand ,  John B. Ohlrogge ,  David N. Kuhn

    发明人： Phillips D. Beremand ,  John B. Ohlrogge ,  David N. Kuhn

    IPC分类号： C07K14/415 ,  C12N1/21 ,  C12N15/54

    CPC分类号： C07K14/415

    摘要： A synthetic gene which encodes for acyl carrier protein (ACP) has been designed. Construction, cloning, and expression in E. coli of spinach ACP-I has been demonstrated. In vitro production of ACP by appropriate expression vectors carrying the synthetic gene will augment the meager supply of this protein. Analogous genes designed for expression of ACP in plants would be a useful tool for controlling fatty acid synthesis and metabolism.

    摘要翻译： 已经设计了编码酰基载体蛋白（ACP）的合成基因。 菠菜ACP-I大肠杆菌中的构建，克隆和表达已被证明。 通过携带合成基因的合适表达载体体外生产ACP将增加该蛋白质的微量供应。 设计用于在植物中表达ACP的类似基因将是控制脂肪酸合成和代谢的有用工具。