公开(公告)号：US5824505A

公开(公告)日：1998-10-20

申请号：US500111

申请日：1995-07-10

申请人： Paul Tolstoshev ,  Richard Harvey ,  Michael Courtney ,  Jean-Pierre LeCocq

发明人： Paul Tolstoshev ,  Richard Harvey ,  Michael Courtney ,  Jean-Pierre LeCocq

IPC分类号： A61K35/16 ,  A61K38/00 ,  A61M1/36 ,  C07K14/815 ,  C07K17/10 ,  C12N1/20 ,  C12N15/00 ,  C12N15/70 ,  C12P21/02 ,  C12Q1/56 ,  C12N1/21 ,  C12N15/63

CPC分类号： C07K14/815 ,  C12N15/70 ,  A61K38/00

摘要： The present invention relates to vectors for cloning, in a host cell, hirudin or an analog of hirudin, characterized in that it comprises the gene for encoding hirudin or an analog of hirudin, and the elements for the expression of this gene in said host cell, said coding gene beginning, after the starting sequence, by an Ile codon and a Thr codon. This invention also relates to the process for the preparation of hirudin or analogs of hirudin as well as the hirudin or anologs of hirudin obtained.

摘要翻译： 本发明涉及用于在宿主细胞，水蛭素或水蛭素类似物中克隆的载体，其特征在于其包含用于编码水蛭素或水蛭素类似物的基因，以及用于在所述宿主细胞中表达该基因的元件 所述编码基因在开始序列之后由Ile密码子和Thr密码子开始。 本发明还涉及水蛭素水蛭素或类似物的制备方法以及获得的水蛭素的水蛭素或阴离子。