公开(公告)号：US20060019254A1

公开(公告)日：2006-01-26

申请号：US10630536

申请日：2003-07-29

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Vladimir Motin

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Vladimir Motin

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/686

摘要： Described herein are the identification of nucleotide sequences specific to Yersinia pestis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 本文描述了用作鉴定该细菌的标记物或签名的鼠疫耶尔森氏菌特异性的核苷酸序列的鉴定。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US20060040268A1

公开(公告)日：2006-02-23

申请号：US10630154

申请日：2003-07-29

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  Y10S435/975

摘要： Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

公开(公告)号：US20060019255A1

公开(公告)日：2006-01-26

申请号：US10630607

申请日：2003-07-29

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/686

摘要： Described herein are the identification of nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

摘要翻译： 本文描述了作为用于鉴定该细菌的标记或签名的布鲁杆菌特异性的核苷酸序列的鉴定。 此外，公开了用于检测细菌存在的核苷酸检测方法中使用的来自这些核苷酸序列的正向和反向引物和杂交探针。

公开(公告)号：US20070111248A1

公开(公告)日：2007-05-17

申请号：US11644669

申请日：2006-12-21

申请人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

发明人： Paula McCready ,  Lyndsay Radnedge ,  Gary Andersen ,  Linda Ott ,  Thomas Slezak ,  Thomas Kuczmarski ,  Elizabeth Vitalis

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/689 ,  Y10S435/975

摘要： Described herein is the identification of nucleotide sequences specific to Francisella tularensis that serves as a marker or signature for identification of this bacterium. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

公开(公告)号：US08232058B2

公开(公告)日：2012-07-31

申请号：US12161291

申请日：2007-01-22

申请人： Mary McBride ,  Thomas Slezak ,  James M. Birch

发明人： Mary McBride ,  Thomas Slezak ,  James M. Birch

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/701 ,  C12Q2531/113

摘要： Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

摘要翻译： 描述了用于检测样品中呼吸道病原体（甲型流感（包括H1，H3，H5和H7亚型的亚型能力）乙型流感，副流感（2型），呼吸道合胞病毒和腺病毒）的试剂盒和方法。 分析来自呼吸道病原体的基因组序列信息以鉴定特征序列，例如可用于确认样品中病原体存在或不存在的多核苷酸序列。 引物和探针组被设计和优化用于基于PCR的多重Luminex测定，以成功鉴定样品中病原体的存在或不存在。

公开(公告)号：US20090305229A1

公开(公告)日：2009-12-10

申请号：US12161291

申请日：2007-01-22

申请人： Mary McBride ,  Thomas Slezak ,  James M. Birch

发明人： Mary McBride ,  Thomas Slezak ,  James M. Birch

IPC分类号： C12Q1/70

CPC分类号： C12Q1/701 ,  C12Q2531/113

摘要： Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

摘要翻译： 描述了用于检测样品中呼吸道病原体（甲型流感（包括H1，H3，H5和H7亚型的亚型能力）乙型流感，副流感（2型），呼吸道合胞病毒和腺病毒）的试剂盒和方法。 分析来自呼吸道病原体的基因组序列信息以鉴定特征序列，例如可用于确认样品中病原体存在或不存在的多核苷酸序列。 引物和探针组被设计和优化用于基于PCR的多重Luminex测定，以成功鉴定样品中病原体的存在或不存在。

公开(公告)号：US20070172835A1

公开(公告)日：2007-07-26

申请号：US11335977

申请日：2006-01-20

申请人： Mary McBride ,  Thomas Slezak ,  James Birch

发明人： Mary McBride ,  Thomas Slezak ,  James Birch

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12M1/34

CPC分类号： C12Q1/70

摘要： The present invention is directed to methods, compositions and kits for multiplex detection of pathogens, such as respiratory pathogens.

摘要翻译： 本发明涉及多种检测病原体如呼吸道病原体的方法，组合物和试剂盒。