-
公开(公告)号:US20090171072A1
公开(公告)日:2009-07-02
申请号:US11875243
申请日:2007-10-19
申请人: Pedro Alfonso , Michael Capaldi , Dennis Dong , Thomas R. Gervais , Joshua Goldstein , Allen B. Magill , Nicole Quinlan , Deepak Saini
发明人: Pedro Alfonso , Michael Capaldi , Dennis Dong , Thomas R. Gervais , Joshua Goldstein , Allen B. Magill , Nicole Quinlan , Deepak Saini
IPC分类号: C07K16/00
CPC分类号: A61K39/39591 , B01D15/3809 , C07K1/22 , C07K2317/52 , C07K2319/30
摘要: Methods useful for producing a solution of purified Fc peptide chains are disclosed. The methods employ protein A chromatography to separate high molecular weight, aggregated peptide chains comprising antibody Fc domains from lower molecular weight unaggregated peptide chains that also comprise antibody Fc domains. The solutions of purified Fc peptide chains obtained by the methods of the invention contain less than 5% aggregate and greater than 70% of the Fc peptide chains subjected to purification.
摘要翻译: 公开了可用于产生纯化的Fc肽链溶液的方法。 该方法采用蛋白A色谱分离从包含抗体Fc结构域的低分子量未聚集肽链分离包含抗体Fc结构域的高分子量聚集肽链。 通过本发明方法获得的纯化的Fc肽链的溶液含有少于5%的聚集体和大于70%的经过纯化的Fc肽链。
-
公开(公告)号:US08853371B2
公开(公告)日:2014-10-07
申请号:US11875243
申请日:2007-10-19
申请人: Pedro Alfonso , Michael Capaldi , Dennis Dong , Thomas R. Gervais , Joshua Goldstein , Allen B. Magill , Nicole Quinlan , Deepak Saini
发明人: Pedro Alfonso , Michael Capaldi , Dennis Dong , Thomas R. Gervais , Joshua Goldstein , Allen B. Magill , Nicole Quinlan , Deepak Saini
IPC分类号: C07K17/00 , B01D15/38 , C07K1/22 , A61K39/395
CPC分类号: A61K39/39591 , B01D15/3809 , C07K1/22 , C07K2317/52 , C07K2319/30
摘要: Methods useful for producing a solution of purified Fc peptide chains are disclosed. The methods employ protein A chromatography to separate high molecular weight, aggregated peptide chains comprising antibody Fc domains from lower molecular weight unaggregated peptide chains that also comprise antibody Fc domains. The solutions of purified Fc peptide chains obtained by the methods of the invention contain less than 5% aggregate and greater than 70% of the Fc peptide chains subjected to purification.
摘要翻译: 公开了可用于产生纯化的Fc肽链溶液的方法。 该方法采用蛋白A色谱分离从包含抗体Fc结构域的低分子量未聚集肽链分离包含抗体Fc结构域的高分子量聚集肽链。 通过本发明方法获得的纯化的Fc肽链的溶液含有少于5%的聚集体和大于70%的经过纯化的Fc肽链。
-