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公开(公告)号：US4720454A
公开(公告)日：1988-01-19
申请号：US601650
申请日：1984-04-18
申请人： Perrin C. White , Bo Dupont , Maria I. New
发明人： Perrin C. White , Bo Dupont , Maria I. New
CPC分类号： C12N9/0087 , C12Q1/6883 , C12Q2600/156 , C12Q2600/172
摘要： A cDNA clone is isolated encoding a bovine adrenal cytochrome P-450 specific for steroid 21-hydroxylation (P-450.sub.C21). This plasmid pC21a contains an insert of 520 base pairs. It hybridizes with mRNA encoding P-450.sub.C21. The peptide encoded by the insert is highly homologous to two peptides isolated from porcine P-450.sub.C21 and shows limited homology to the P-450 induced by phenobarbital in rat liver. This probe and its human equivalents can be used to screen in utero i.e. pre-natal as well as post-natal patients for adrenal hyperplasia since it reacts well with human DNA as shown. These probes can also be useful for an industrial fermentation process to produce cortisone in quantity.
摘要翻译： 分离cDNA克隆，其编码对类固醇21-羟基化（P-450C21）特异性的牛肾上腺细胞色素P-450。 该质粒pC21a含有520个碱基对的插入片段。 它与编码P-450C21的mRNA杂交。 由插入片段编码的肽与从猪P-450C21分离的两种肽高度同源，并且与大鼠肝脏中苯巴比妥诱导的P-450显示有限的同源性。 该探针及其人类等同物可用于筛选子宫内，即出生前以及出生后患者的肾上腺增生，因为如图所示，它与人类DNA发生反应良好。 这些探针也可用于工业发酵过程以量产生可的松。
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公开(公告)号：US4230684A
公开(公告)日：1980-10-28
申请号：US887326
申请日：1978-03-16
申请人： Songja Pang , Maria I. New
发明人： Songja Pang , Maria I. New
CPC分类号： G01N33/76 , Y10S436/811 , Y10S436/817 , Y10S436/825
摘要： A micromethod for the determination of steroids in human body liquids, in particular blood, is disclosed. A small sample of the liquid is transferred to a sheet of absorbing material, e.g., filter paper. A disc of the impregnated paper, containing the dried sample, in eluted with an aqueous solvent, e.g., a buffer solution. Without separating the paper from the elute, the latter is extracted with an organic solvent. A steroid-containing residue is recovered from the organic extract and is subjected to a conventional radioimmunoassay. The method is particularly suited for the detection of disorders with elevated steroid levels such as congenital adrenal hyperplasia in newborn infants by determining the 17.alpha.-hydroxy-progesterone concentration in small blood samples.
摘要翻译： 公开了用于测定人体液体，特别是血液中类固醇的微量方法。 将一小部分液体样品转移到一张吸收材料（例如滤纸）上。 含有干燥样品的浸渍纸盘，用水溶剂例如缓冲溶液洗脱。 不将纸与洗脱液分离，后者用有机溶剂萃取。 从有机提取物中回收含类固醇的残留物，并进行常规的放射免疫测定。 该方法特别适用于通过测定小血液样品中17α-羟基孕酮浓度来检测新生儿中先天性肾上腺增生的类固醇水平升高的疾病。
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