公开(公告)号：US5866399A

公开(公告)日：1999-02-02

申请号：US695434

申请日：1996-08-12

申请人： Peter Crause ,  Paul Habermann ,  Jorg Moller ,  Wolfgang Ulmer

发明人： Peter Crause ,  Paul Habermann ,  Jorg Moller ,  Wolfgang Ulmer

IPC分类号： C12N9/48 ,  C07K14/815 ,  C12N1/19 ,  C12N9/99 ,  C12P21/00 ,  C12P21/02 ,  C12R1/865 ,  C12N9/50 ,  A23J1/00 ,  C12M1/32 ,  C12N1/14

CPC分类号： C12P21/02 ,  C07K14/815

摘要： The present invention relates to a process for inactivating Carboxypeptidase Y (CPY) in a hirudin-containing culture broth produced by fermenting a transformed yeast. It has been found that inactive precursors of CPY are activated at a temperature 70.degree. C. and that the active peptidases will further degrade hirudin when the two proteins are present together in a composition. The present invention overcomes the CPY activation problem by heating a broth containing hirudin and CPY to a temperature of about 80.degree.-100.degree. C. in about one minute or less.

摘要翻译： 本发明涉及通过发酵转化的酵母产生的含水蛭素的培养液中灭活羧肽酶Y（CPY）的方法。 已经发现，CPY的无活性前体在70℃的温度下被活化，并且当两种蛋白质在组合物中一起存在时，活性肽酶将进一步降解水蛭素。 本发明通过在约1分钟或更短的时间内将含有水蛭素和CPY的肉汤加热至约80℃-100℃的温度来克服CPY活化问题。

公开(公告)号：US20070154585A1

公开(公告)日：2007-07-05

申请号：US11642966

申请日：2006-12-21

申请人： Jorg Schmuhl ,  Brigitte Diekhaus ,  Rolf Michels ,  Jorg Moller

发明人： Jorg Schmuhl ,  Brigitte Diekhaus ,  Rolf Michels ,  Jorg Moller

IPC分类号： B29C47/92

CPC分类号： B29C48/907 ,  B29C48/07 ,  B29C48/09 ,  B29C48/903 ,  B29C48/905 ,  B29C48/908 ,  B29C48/913 ,  B29C48/919

摘要： Calibration sleeve for extruded plastic pipes with a first layer of flexible straps that run diagonal to the longitudinal axis of the calibration device and a second layer of flexible straps, the first and second layers of straps intercross in the manner of an extendable lattice grate in which the intercrossing points are connected in an articulated manner. The first or second layers of straps are fastened in an articulated manner on an axially adjustable rigid retaining ring at the end of a calibration sleeve, and the straps of the other layer end at an axial distance from the retaining ring. Such a calibration sleeve has an inside diameter which can be adjusted precisely while taking the contraction behavior of the extruded pipe into account. The straps of the first and second layer are fastened in a stationary and in an articulated manner at the intake of the calibration sleeve.

摘要翻译： 用于挤出塑料管的校准套管，其具有与校准装置的纵向轴线对角延伸的第一层柔性带和第二层柔性带，第一和第二层带以可延伸的格栅格栅的方式交叉，其中 交叉点以铰接方式连接。 第一层或第二层带以铰接的方式紧固在校准套筒末端的可轴向调节的刚性保持环上，而另一层的带束在与保持环的轴向距离处终止。 这种校准套筒具有可以精确调节的内径，同时考虑到挤出管的收缩特性。 第一层和第二层的带子以固定的方式并且以铰接方式固定在校准套筒的入口处。

公开(公告)号：US6103502A

公开(公告)日：2000-08-15

申请号：US755114

申请日：1996-11-22

申请人： Jorg Moller ,  Frank Richard

发明人： Jorg Moller ,  Frank Richard

IPC分类号： B01D61/14 ,  C07K1/14 ,  C07K1/34 ,  C07K14/815 ,  C12P21/04 ,  C12P1/00 ,  C12P21/06

CPC分类号： C07K1/34 ,  C07K14/815 ,  Y10S435/814

摘要： A peptide such as in a cell-free fermentation medium is ultrafiltered with a membrane having a molecular weight cut-off of at least approximately two times greater than the molecular weight of the peptide. The peptide is retained by the membrane, and is desalinated and concentrated to provide prepurification of the peptide. A preferred temperature for ultrafiltration is about 5 to about 15.degree. C. Permeate flow rate may be about 10 l/m.sup.2 /h to about 35 l/m.sup.2 /h, and permeate conductivity may be less than about 10 mS/cm. The permeate may be recycled until peptide concentration is constant. A cell-free fermentation medium containing hirudin from a recombinant microorganism such as Saccharomyces cerevisiae is ultrafiltered with a membrane having a molecular weight cut-off of about 20 kD to about 30 kD.

摘要翻译： 诸如在无细胞发酵培养基中的肽用分子量截留值至少大约为肽的分子量的大约2倍的膜进行超滤。 肽被膜保留，并脱盐并浓缩以提供肽的预纯化。 超滤的优选温度为约5至约15℃。渗透流速可以为约10l / m 2 / h至约35l / m 2 / h，并且渗透物导电率可以小于约10mS / cm。 渗透物可以循环使用，直到肽浓度恒定。 含有重组微生物如酿酒酵母的含水蛭素的无细胞发酵培养基用分子量截留约20kD至约30kD的膜进行超滤。