公开(公告)号：US20180291412A1

公开(公告)日：2018-10-11

申请号：US16014348

申请日：2018-06-21

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  David C. Watkins ,  Stacey L. Lee ,  Frank H. Gaertner ,  Robert Shutter

IPC分类号： C12P21/00 ,  C07K14/61 ,  C07K14/52 ,  C12P21/02 ,  C12N15/78 ,  C07K16/40 ,  C07K14/57

摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.

公开(公告)号：US10041102B2

公开(公告)日：2018-08-07

申请号：US15230192

申请日：2016-08-05

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  David C. Watkins ,  Stacey L. Lee ,  Frank H. Gaertner ,  Robert Shutter

IPC分类号： C12P21/02 ,  C12P21/06 ,  C07K14/52 ,  C12P21/00 ,  C07K16/40 ,  C12N15/78 ,  C07K14/57 ,  C07K14/61

摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances. Processes for improved production of isolated mammalian, particularly human, proteins are provided.

公开(公告)号：US20170051327A1

公开(公告)日：2017-02-23

申请号：US15230192

申请日：2016-08-05

申请人： Pfenex Inc.

发明人： Diane M. Retallack ,  Charles H. Squires ,  David C. Watkins ,  Stacey L. Lee ,  Frank H. Gaertner ,  Robert Shutter

IPC分类号： C12P21/00 ,  C12N15/78 ,  C07K14/61 ,  C07K16/40 ,  C07K14/57

CPC分类号： C12P21/00 ,  C07K14/52 ,  C07K14/57 ,  C07K14/61 ,  C07K16/40 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/78 ,  C12P21/02 ,  C12Y302/01023

摘要： The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable Circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.

摘要翻译： 本发明是通过在假单胞菌，特别是荧光假单胞菌生物体中表达来改进重组哺乳动物蛋白质生产的方法。 本方法提供了哺乳动物蛋白质，特别是人或人衍生的蛋白质，通过已知的表达系统如大肠杆菌在可比较的情况下改进生产分离的哺乳动物，特别是人类蛋白质的方法。