公开(公告)号：US20090118248A1

公开(公告)日：2009-05-07

申请号：US11587138

申请日：2005-04-22

申请人： Ping W. Chang ,  Ning Zhong ,  Xinping Fang ,  Shao-Kun Pang ,  Chi-Nung Hsiao ,  Tsung-Cheng Hu

发明人： Ping W. Chang ,  Ning Zhong ,  Xinping Fang ,  Shao-Kun Pang ,  Chi-Nung Hsiao ,  Tsung-Cheng Hu

IPC分类号： A61K31/58 ,  C07J7/00 ,  A61P25/00 ,  C07J43/00

CPC分类号： C07J43/003 ,  C07J7/002 ,  C07J7/0085

摘要： The invention relates to a novel multi step process of making compounds of Formula I: wherein R1 is an alkoxy group and R2 is an optionally substituted, N-attached heteroaryl. The hydrogen at the 5-position can be α or β isomer, preferably α. Preferably the compound of Formula I is 17β isomer. The invention also relates to novel 3α-hydroxy-3β-substituted-17-substituted steroid compounds having GABAA receptor modulating activity, pharmaceutical compositions comprising these compounds, and the use of these compounds in a method of modulating brain excitability.

摘要翻译： 本发明涉及制备式I化合物的新型多步骤方法：其中R 1是烷氧基，R 2是任选取代的，N-连接的杂芳基。 5位氢可以是α或β异构体，最好是α。 优选地，式I的化合物是17β型异构体。 本发明还涉及具有GABA A受体调节活性的新的3α-羟基-3β-取代的-17-取代的类固醇化合物，包含这些化合物的药物组合物，以及这些化合物在调节脑兴奋性的方法中的用途。

公开(公告)号：US20080319163A1

公开(公告)日：2008-12-25

申请号：US11911201

申请日：2006-02-14

申请人： Yuliang Wang ,  Qinghai Sheng ,  Xinping Fang ,  Wenshui Xia ,  Hongmei Zhai ,  Peng Wei

发明人： Yuliang Wang ,  Qinghai Sheng ,  Xinping Fang ,  Wenshui Xia ,  Hongmei Zhai ,  Peng Wei

IPC分类号： C07K7/06

CPC分类号： A61K35/50 ,  A61K38/00 ,  C07K14/4715

摘要： The present invention provides a method for isolating and purifying immuno-modulating polypeptide from cow placenta, which is characterized by using the steps of anion-exchange chromatography, gel exclusion chromatography and reverse-phase high performance liquid chromatography to isolate and purify immuno-modulating polypeptide from cow placenta, identifying its activity of stimulating lymphocyte proliferation in vitro by MTT method, then determining its molecular weight by MALDI-TOF-MS, its isoelectric point by CIEF and its amino acid sequence with analyzer for protein sequencing. Since the obtained immuno-modulating polypeptide by the method according to the present invention has more than 90% purity, its bioactivity can reach medicinal standards.

摘要翻译： 本发明提供了一种从牛胎盘中分离和纯化免疫调节多肽的方法，其特征在于使用阴离子交换色谱法，凝胶排阻色谱法和反相高效液相色谱法分离和纯化免疫调节多肽 从牛胎盘中，通过MTT法鉴定其在体外刺激淋巴细胞增殖的活性，然后通过MALDI-TOF-MS测定其分子量，通过CIEF测定其等电点及其氨基酸序列与蛋白质测序分析仪。 由于通过本发明的方法获得的免疫调节多肽具有超过90％的纯度，因此其生物活性可达到医学标准。