公开(公告)号：US20210345618A1

公开(公告)日：2021-11-11

申请号：US17278022

申请日：2019-09-19

申请人： Pivot Bio, Inc.

发明人： Sarah Bloch ,  Karsten Temme ,  Alvin Tamsir ,  Douglas Higgins ,  Austin Davis-Richardson ,  Rosemary Clark ,  Shayin Gottlieb ,  James Gerard Lorigan

IPC分类号： A01N63/20 ,  C12N15/01 ,  A01N63/30 ,  C12N1/14 ,  C12N1/20

摘要： Methods and systems are provided for increasing the phosphate solubilization activity of a microbe. Microbes identified through the use of the disclosed methods and systems are also provided. The methods include the steps of and the systems include instructions for identifying a coding sequence associated with phosphate solubilization, altering the coding sequence associated with phosphate solubilization, functionally linking the altered coding sequence associated with phosphate solubilization to a promoter, and introducing the altered coding sequence associated with phosphate solubilization under the control of the promoter into the microbe. Alterations to the coding sequence can include, e.g., codon randomization of native codons, deletion of coding sequence, and insertion of regulatory sequences.