公开(公告)号：US20210009483A1

公开(公告)日：2021-01-14

申请号：US17027030

申请日：2020-09-21

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung ,  Kevin Hammill ,  Douglas Higgins ,  Austin Davis-Richardson

IPC分类号： C05F11/08 ,  C12N1/20 ,  C12R1/22 ,  C12R1/025 ,  A01H3/00 ,  C12R1/01 ,  A01N63/00 ,  A01N63/10 ,  A01H6/46 ,  A01H5/06 ,  C12N1/04 ,  C12N15/11 ,  C12N15/52 ,  C12N15/74 ,  C12R1/065 ,  C12R1/07 ,  C12N15/70

摘要： Methods and systems are provided for generating and utilizing a bacterial composition that comprises at least one genetically engineered bacterial strain that fixes atmospheric nitrogen in an agricultural system that has been fertilized with more than 20 lbs of Nitrogen per acre.

公开(公告)号：US20220017911A1

公开(公告)日：2022-01-20

申请号：US17312172

申请日：2019-12-20

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Neal Shah ,  Shayin Gottlieb ,  Richard Broglie ,  Emily Tung ,  Rosemary Clark

IPC分类号： C12N15/74 ,  C12N1/20 ,  C12Q1/04

摘要： Methods of predicting in planta phenotypes of microbial strains are provided. The methods can include culturing microbial strains in a plant exudate medium and assaying in vitro phenotypes of the microbial strains. The methods may also include using the in vitro phenotypes to predict in planta phenotypes of the microbial strains. Methods of using the microbial strains in field trials are also provided.

公开(公告)号：US10934226B2

公开(公告)日：2021-03-02

申请号：US15954558

申请日：2018-04-16

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C07K14/195 ,  C05C1/00 ,  C05C3/00 ,  C05C5/00 ,  C05C11/00 ,  C12N9/02 ,  C12N9/80 ,  C12N9/00 ,  C12N1/20

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

公开(公告)号：US10556839B2

公开(公告)日：2020-02-11

申请号：US15954557

申请日：2018-04-16

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C07K14/195 ,  C05C1/00 ,  C05C3/00 ,  C05C5/00 ,  C05C11/00 ,  C12N9/02 ,  C12N9/80 ,  C12N9/00 ,  C12N1/20

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

公开(公告)号：US20210345618A1

公开(公告)日：2021-11-11

申请号：US17278022

申请日：2019-09-19

申请人： Pivot Bio, Inc.

发明人： Sarah Bloch ,  Karsten Temme ,  Alvin Tamsir ,  Douglas Higgins ,  Austin Davis-Richardson ,  Rosemary Clark ,  Shayin Gottlieb ,  James Gerard Lorigan

IPC分类号： A01N63/20 ,  C12N15/01 ,  A01N63/30 ,  C12N1/14 ,  C12N1/20

摘要： Methods and systems are provided for increasing the phosphate solubilization activity of a microbe. Microbes identified through the use of the disclosed methods and systems are also provided. The methods include the steps of and the systems include instructions for identifying a coding sequence associated with phosphate solubilization, altering the coding sequence associated with phosphate solubilization, functionally linking the altered coding sequence associated with phosphate solubilization to a promoter, and introducing the altered coding sequence associated with phosphate solubilization under the control of the promoter into the microbe. Alterations to the coding sequence can include, e.g., codon randomization of native codons, deletion of coding sequence, and insertion of regulatory sequences.

公开(公告)号：US20210214282A1

公开(公告)日：2021-07-15

申请号：US17148173

申请日：2021-01-13

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C07K14/195 ,  C05C1/00 ,  C05C3/00 ,  C05C5/00 ,  C05C11/00 ,  C12N9/02 ,  C12N9/80 ,  C12N9/00 ,  C12N1/20

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

公开(公告)号：US10919814B2

公开(公告)日：2021-02-16

申请号：US16685997

申请日：2019-11-15

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C12N9/02 ,  C12N9/80 ,  C05C1/00 ,  C05C3/00 ,  C05C5/00 ,  C05C11/00 ,  C07K14/195 ,  C12N1/20 ,  C12N9/22 ,  C12N15/74 ,  C05G3/90 ,  C12N9/00

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

公开(公告)号：US10384983B2

公开(公告)日：2019-08-20

申请号：US15950534

申请日：2018-04-11

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C07K14/195 ,  C12N1/20 ,  C05C1/00 ,  C05C3/00 ,  C05C5/00 ,  C05C11/00 ,  C12N9/02 ,  C12N9/80 ,  C12N9/00

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

公开(公告)号：US20240010576A1

公开(公告)日：2024-01-11

申请号：US18345783

申请日：2023-06-30

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C07K14/195 ,  C05C1/00 ,  C05C3/00 ,  C05C5/00 ,  C05C11/00 ,  C12N9/02 ,  C12N9/80 ,  C12N9/00 ,  C12N1/20

CPC分类号： C05F11/08 ,  C07K14/195 ,  C05C1/00 ,  C05C3/005 ,  C05C5/005 ,  C05C11/00 ,  C12N9/0095 ,  C12N9/80 ,  C12N9/93 ,  C12Y118/06001 ,  C12Y305/01002 ,  C12Y603/01002 ,  C12N1/20

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.

公开(公告)号：US11739032B2

公开(公告)日：2023-08-29

申请号：US17148173

申请日：2021-01-13

申请人： Pivot Bio, Inc.

发明人： Karsten Temme ,  Alvin Tamsir ,  Sarah Bloch ,  Rosemary Clark ,  Emily Tung

IPC分类号： C05F11/08 ,  C05C1/00 ,  C05C3/00 ,  C05C11/00 ,  C07K14/195 ,  C12N1/20 ,  C12N9/02 ,  C12N9/80 ,  C12N9/00 ,  C12N15/52 ,  C12N15/74 ,  C05G3/90 ,  C12N15/63 ,  C05C5/00

CPC分类号： C05F11/08 ,  C05C1/00 ,  C05C3/005 ,  C05C5/005 ,  C05C11/00 ,  C07K14/195 ,  C12N1/20 ,  C12N9/0095 ,  C12N9/80 ,  C12N9/93 ,  C12Y118/06001 ,  C12Y305/01002 ,  C12Y603/01002 ,  C05C1/00 ,  C05F11/08 ,  C05C3/005 ,  C05F11/08 ,  C05C5/005 ,  C05F11/08 ,  C05C11/00 ,  C05F11/08

摘要： Disclosed herein are methods of increasing nitrogen fixation in a non-leguminous plant. The methods can comprise exposing the plant to a plurality of bacteria. Each member of the plurality comprises one or more genetic variations introduced into one or more genes or non-coding polynucleotides of the bacteria's nitrogen fixation or assimilation genetic regulatory network, such that the bacteria are capable of fixing atmospheric nitrogen in the presence of exogenous nitrogen. The bacteria are not intergeneric microorganisms. Additionally, the bacteria, in planta, produce 1% or more of the fixed nitrogen in the plant.