公开(公告)号：US20250122518A1

公开(公告)日：2025-04-17

申请号：US18897539

申请日：2024-09-26

Applicant: PlantForm Corporation

Inventor： Michael D. McLean ,  John D. Cossar ,  Wing-Fai Cheung ,  Haifeng Wang

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/12 ,  C12P21/00

Abstract: Plant T-DNA expression vectors encoding a post-translational modification (PTM) enzyme wherein the nucleic acid encoding the PTM enzyme has neither a promoter nor a 5′ untranslated region (UTR) are provided. Also provided are methods of optimizing expression and glycosylation of recombinant protein produced in plants by utilizing the plant T-DNA expression vectors.

公开(公告)号：US11802289B2

公开(公告)日：2023-10-31

申请号：US16635402

申请日：2018-08-02

Applicant: PlantForm Corporation

Inventor： Kiva Ferraro ,  Michael Marit ,  Michael D. McLean

IPC: C12N15/82 ,  C07K16/32

CPC classification number: C12N15/8218 ,  C07K16/32 ,  C12N15/8258 ,  C07K2317/13 ,  C07K2317/24

Abstract: A plant or plant cell with reduced endogenous AGO1 and AGO4 expression compared to a wild-type plant or plant cell is provided. In one embodiment, the plant or plant cell further comprises a nucleic acid sequence encoding a recombinant protein and the plant or plant cell has increased expression of the recombinant protein compared to a wild-type plant or plant cell comprising the nucleic acid sequence. Methods of reducing aglycosylation of recombinant protein produced in plants are also provided.

公开(公告)号：US20250122519A1

公开(公告)日：2025-04-17

申请号：US18897661

申请日：2024-09-26

Applicant: PlantForm Corporation

Inventor： Michael D. McLean ,  John D. Cossar ,  Wing-Fai Cheung ,  Haifeng Wang

IPC: C12N15/82 ,  C12N9/10 ,  C12N9/12 ,  C12P21/00

Abstract: Plant T-DNA expression vectors encoding a post-translational modification (PTM) enzyme wherein the nucleic acid encoding the PTM enzyme has neither a promoter nor a 5′ untranslated region (UTR) are provided. Also provided are methods of optimizing expression and glycosylation of recombinant protein produced in plants by utilizing the plant T-DNA expression vectors.

公开(公告)号：US11499160B2

公开(公告)日：2022-11-15

申请号：US16464818

申请日：2017-11-29

Applicant: PlantForm Corporation

Inventor： Michael D. McLean ,  Zacharie LeBlanc

IPC: C12N15/82 ,  C12N9/10

Abstract: A genetically modified plant or plant cell with reduced α1,3-fucosyltransferase and β1,2-xylosyltransferase activity compared to a wild type plant or plant cell, wherein less than 10% of the total glycan on a protein produced by the plant or plant cell is α1,3-fucosylated glycan and less than 3% of the total glycan on the protein is β1,2-xylosylated glycan is provided. In one embodiment, the plant or plant cell comprises three T-DNA insertions expressing five copies of RNAi targeting α1,3-fucosyltranserase and three copies of RNAi targeting β1,2xylosyltransferase.

公开(公告)号：US20220135992A1

公开(公告)日：2022-05-05

申请号：US17435946

申请日：2020-02-27

Applicant: PlantForm Corporation

Inventor： Michael D. McLean ,  John D. Cossar ,  Wing-Fai Cheung ,  Haifeng Wang

IPC: C12N15/82 ,  C12N9/12 ,  C12P21/00

Abstract: Plant T-DNA expression vectors with engineered 5′ sequences for driving transcription of genes encoding post-translational modification enzymes are provided. Also methods of optimizing expression and glycosylation of recombinant protein produced in plants by utilizing plant T-DNA expression vectors with engineered 5′ sequences for driving transcription of genes encoding post-translational modification enzymes.