公开(公告)号：US20140302486A1

公开(公告)日：2014-10-09

申请号：US14139464

申请日：2013-12-23

申请人： President and Fellows of Harvard College ,  University of Washington Through Its Center For Commercialization

发明人： Georg Seelig ,  Xi Chen ,  David Yu Zhang

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q1/6876 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q2600/178 ,  C12Q2537/1373 ,  C12Q2537/155 ,  C12Q2537/157 ,  C12Q2565/625 ,  C12Q2565/101

摘要： A detection probe for detecting single base mutations or alterations in a double stranded target nucleic acid molecule is provided. In some aspects, the detection probe may include a double-stranded probe nucleic acid molecule having a first end and a second end; at least one probe initiation toehold at the first end; at least one substance capable of emitting a detectable signal at the second end; and optionally, at least one dissociation toehold at the second end. The detection probe is designed to hybridize with a double stranded target nucleic acid in a reaction which proceeds at approximate thermodynamic equilibrium (ΔG≈0) when no single base mutations or alterations are present in the double stranded target nucleic acid molecule. The probe may be used in detection systems and methods to identifying the presence or absence of one or more single base mutations or alterations in a double-stranded nucleic acid target molecule.

摘要翻译： 提供了用于检测双链靶核酸分子中的单碱基突变或改变的检测探针。 在一些方面，检测探针可以包括具有第一末端和第二末端的双链探针核酸分子; 在第一端至少有一个探头起始; 能够在第二端发射可检测信号的至少一种物质; 并且任选地，在第二端处至少一个离解至止。 设计检测探针与当双链靶核酸分子中没有单碱基突变或改变存在时，以近似热力学平衡（＆Dgr;G≈0）进行的反应中的双链靶核酸杂交。 探针可以用于检测系统和方法中以鉴定双链核酸靶分子中一个或多个单碱基突变或改变的存在或不存在。