公开(公告)号：US12006509B2

公开(公告)日：2024-06-11

申请号：US16896278

申请日：2020-06-09

Applicant: Purdue Research Foundation

Inventor： Sophie Andree Lelievre ,  Rahim Rahimi ,  Tim Kwok

IPC: C12N5/00 ,  C12M1/12 ,  C12N5/09

CPC classification number: C12N5/0068 ,  C12M25/14 ,  C12N5/0062 ,  C12N5/0693 ,  C12N2533/30 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

Abstract: A collagen-fibronectin-based method enables the production of tumors of centimeter size with recognizable pathological traits. The method supports reproducing tumor heterogeneity with preinvasive and invasive phenotypes and stacking of tumor portions using a paper-scaffold with 80 μm-punched holes for larger nodule creation and easy separation of tumor portions for analysis. Macrotumors are convenient for testing drug delivery and therapeutic tools that necessitate a minimum tumor size relevant to in vivo.