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公开(公告)号：US20210171967A1
公开(公告)日：2021-06-10
申请号：US16770906
申请日：2018-12-06
IPC分类号： C12N15/82 , C12N15/113
摘要： A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.
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公开(公告)号：US11773401B2
公开(公告)日：2023-10-03
申请号：US16770906
申请日：2018-12-06
IPC分类号： C12N15/82 , C12N15/113 , A01H5/12
CPC分类号： C12N15/8218 , A01H5/12 , C12N15/113 , C12N2310/11 , C12N2310/141 , C12N2310/3231
摘要： A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.
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公开(公告)号：US20220213496A1
公开(公告)日：2022-07-07
申请号：US17605506
申请日：2020-06-05
IPC分类号： C12N15/82 , C07K14/415
摘要： A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.
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公开(公告)号：US20150313276A9
公开(公告)日：2015-11-05
申请号：US13940925
申请日：2013-07-12
IPC分类号： A24B15/42
CPC分类号： A24B15/42 , A24B15/28 , A24B15/30 , A24B15/302
摘要： A method of curing tobacco. The method comprises the steps of contacting a tobacco material with a curing additive comprising one or more salts and curing the tobacco material under conditions and temperature for a time sufficient to produce cured tobacco wherein the amount of the one or more salts contacted with the tobacco is at least 5 percent of the dry weight of the cured tobacco.
摘要翻译： 一种治疗烟草的方法。 该方法包括以下步骤：使烟草材料与包含一种或多种盐的固化添加剂接触，并在条件和温度下固化烟草材料足以产生固化烟草的时间，其中与烟草接触的一种或多种盐的量为 至少5％的干燥烟草干重。
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公开(公告)号：US20230416766A1
公开(公告)日：2023-12-28
申请号：US18243312
申请日：2023-09-07
IPC分类号： C12N15/82 , C12N15/113 , A01H5/12
CPC分类号： C12N15/8218 , C12N15/113 , A01H5/12 , C12N2310/11 , C12N2310/141 , C12N2310/3231
摘要： A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region/leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.
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公开(公告)号：US11649465B2
公开(公告)日：2023-05-16
申请号：US16127818
申请日：2018-09-11
IPC分类号： C12N15/82 , C07K14/535 , C07K16/18 , C12N15/113
CPC分类号： C12N15/8258 , C07K14/535 , C07K16/18 , C12N15/113 , C12N15/8205 , C12N15/8209 , C12N15/8216 , C12N15/8243 , C12N15/8257
摘要： Methods and compositions are provided for increasing the expression of a gene of interest in a plant by co-expressing the gene of interest with a p21 polynucleotide. The gene of interest can be endogenous or heterologous to the plant. Further provided are plants, such as tobacco plants, comprising a heterologous p21 polynucleotide. Co-expression of the p21 polynucleotide with a gene of interest in the plant increases the expression of the gene of interest when compared to a control plant. Accordingly, p21 co-expression can increase the expression of genes of interest encoding proteins such as defense proteins, enzymes, signaling proteins, reporter proteins, antibodies and fragments thereof, growth factors, cell surface receptor molecules, seed storage proteins, and fungicides in a plant.
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公开(公告)号：US20160128294A1
公开(公告)日：2016-05-12
申请号：US14537181
申请日：2014-11-10
CPC分类号： A24B13/00 , A24B15/303 , A24D3/041 , A24D3/06
摘要： Treated plant seeds are provided, which have one or more modifying ingredients associated therewith. Such modifying ingredients can include compositions comprising water, flavorants, pH adjusters, buffering agents, humectants, antioxidants, oral care ingredients, preservatives, additives derived from herbal or botanical sources, and mixtures thereof that can be provided or diluted form. The treated plant seeds can be incorporated within tobacco products, including smoking articles, to alter the properties thereof (e.g., by releasing the one or more modifying ingredients into the mainstream smoke of a smoking article).
摘要翻译： 提供了处理的植物种子，其具有与其相关的一种或多种改性成分。 这种改性成分可以包括可以提供或稀释形式的包含水，调味剂，pH调节剂，缓冲剂，保湿剂，抗氧化剂，口腔护理成分，防腐剂，衍生自草药或植物来源的添加剂的组合物，及其混合物。 经处理的植物种子可以并入烟草制品中，包括吸烟制品，以改变其性质（例如通过将一种或多种改性成分释放到吸烟制品的主流烟雾中）。
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公开(公告)号：US20150013694A1
公开(公告)日：2015-01-15
申请号：US13940925
申请日：2013-07-12
IPC分类号： A24B15/42
CPC分类号： A24B15/42 , A24B15/28 , A24B15/30 , A24B15/302
摘要： A method of curing tobacco. The method comprises the steps of contacting a tobacco material with a curing additive comprising one or more salts and curing the tobacco material under conditions and temperature for a time sufficient to produce cured tobacco wherein the amount of the one or more salts contacted with the tobacco is at least 5 percent of the dry weight of the cured tobacco.
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公开(公告)号：US20230159945A1
公开(公告)日：2023-05-25
申请号：US17995675
申请日：2021-04-09
CPC分类号： C12N15/8243 , C12N15/8218 , A24B15/10 , A24B13/00 , A24F40/20
摘要： The present invention provides a method for modulating (e.g. decreasing) the nicotine content of a plant (e.g. a tobacco plant) or part thereof, or tobacco plant cell, the method comprising modifying said plant or cell providing at least one mutation in a Nic3 locus. The present invention provides a method for modulating (e.g. decreasing) the nicotine content of a plant (e.g. a tobacco plant) or part thereof, or tobacco plant cell, the method comprising modifying said plant or cell to modulate the expression or activity of at least one Nic3 gene. The present invention also provides for the use of the Nic3 locus for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or delivery systems obtainable in accordance with the invention.
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10.发明申请公开(公告)号：US20190093119A1
公开(公告)日：2019-03-28
申请号：US16127818
申请日：2018-09-11
IPC分类号： C12N15/82 , C12N15/113 , C07K16/18 , C07K14/535
摘要： Methods and compositions are provided for increasing the expression of a gene of interest in a plant by co-expressing the gene of interest with a p21 polynucleotide. The gene of interest can be endogenous or heterologous to the plant. Further provided are plants, such as tobacco plants, comprising a heterologous p21 polynucleotide. Co-expression of the p21 polynucleotide with a gene of interest in the plant increases the expression of the gene of interest when compared to a control plant. Accordingly, p21 co-expression can increase the expression of genes of interest encoding proteins such as defense proteins, enzymes, signaling proteins, reporter proteins, antibodies and fragments thereof, growth factors, cell surface receptor molecules, seed storage proteins, and fungicides in a plant.
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