公开(公告)号：US20220315644A1

公开(公告)日：2022-10-06

申请号：US17843121

申请日：2022-06-17

Applicant: REGENERON PHARMACEUTICALS, INC.

Inventor： Shawn LAWRENCE ,  Michelle LAFOND ,  Ann KIM ,  Amy S. JOHNSON ,  Brandon VERES ,  Mark CZETERKO ,  Kristen WHALEN

IPC: C07K16/00 ,  C12Q3/00 ,  C12P21/02

Abstract: Improved methods for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.