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    CELL CULTURE PROCESS FOR MAKING A GLYCOPROTEIN
    2.
    发明申请
    CELL CULTURE PROCESS FOR MAKING A GLYCOPROTEIN 有权

    公开(公告)号：US20210388408A1

    公开(公告)日：2021-12-16

    申请号：US17409193

    申请日：2021-08-23

    Applicant: Regeneron Pharmaceuticals, Inc.

    Inventor： John CHEN ,  Shawn LAWRENCE ,  Amy JOHNSON ,  Theodore LONEY ,  Ravindra PANGULE ,  Ta-Chun HANG ,  Scott CARVER ,  Bernhard SCHILLING

    IPC: C12P21/00 ,  C07K14/47 ,  C07K14/715 ,  C07K14/71

    Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

    METABOLICALLY OPTIMIZED CELL CULTURE
    4.
    发明申请
    METABOLICALLY OPTIMIZED CELL CULTURE 有权

    公开(公告)号：US20210087535A1

    公开(公告)日：2021-03-25

    申请号：US16984531

    申请日：2020-08-04

    Applicant: Regeneron Pharmaceuticals, Inc.

    Inventor： Shawn LAWRENCE ,  Ann KIM ,  Amy S. JOHNSON

    IPC: C12N5/071 ,  C12N5/00 ,  C12P21/02 ,  C07K16/00

    Abstract: An improved method for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

    CELL CULTURE PROCESS FOR MAKING A GLYCOPROTEIN
    6.
    发明申请
    CELL CULTURE PROCESS FOR MAKING A GLYCOPROTEIN 有权

    公开(公告)号：US20210388407A1

    公开(公告)日：2021-12-16

    申请号：US17409158

    申请日：2021-08-23

    Applicant: Regeneron Pharmaceuticals, Inc.

    Inventor： John CHEN ,  Shawn LAWRENCE ,  Amy JOHNSON ,  Theodore LONEY ,  Ravindra PANGULE ,  Ta-Chun HANG ,  Scott CARVER ,  Bernhard SCHILLING

    IPC: C12P21/00 ,  C07K14/47 ,  C07K14/715 ,  C07K14/71

    Abstract: The instant application provides a method for screening batches of soy hydrolysate for a desired amount of a component thereof, such as ornithine or putrescine, and selecting only those batches of soy hydrolysate that have a desired amount of such component. The present disclosure also sets forth methods for culturing cells in media supplemented with selected batches of soy to produce more consistent, high quality lots of a protein of interest. Further, the present disclosure provides a plurality of protein preparations that have each been produced by culturing cells in media supplemented with separate batches of soy hydrolysate containing a desired amount of ornithine or putrescine, whereby each batch of protein produced exhibits improved quality of the protein of interest or amount of quality protein produced.

    METABOLICALLY OPTIMIZED CELL CULTURE
    8.
    发明申请
    METABOLICALLY OPTIMIZED CELL CULTURE 审中-公开
    代谢优化细胞培养

    公开(公告)号：US20160244725A1

    公开(公告)日：2016-08-25

    申请号：US15028521

    申请日：2014-10-10

    Applicant: REGENERON PHARMACEUTICALS, INC.

    Inventor： Shawn LAWRENCE ,  Ann KIM ,  Amy S. JOHNSON

    IPC: C12N5/071

    CPC classification number: C12N5/0682 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/00 ,  C12N2500/60 ,  C12N2511/00 ,  C12P21/02

    Abstract: An improved method for large scale production of proteins and/or polypeptides in cell culture is provided. In accordance with the present invention, the method provides for culturing cells that have metabolically shifted. The use of such a method or system allows high levels of protein or polypeptide production and reduces accumulation of unwanted metabolic waste such as lactate. Proteins and polypeptides expressed in accordance with the present invention may be advantageously used in the preparation of pharmaceutical, immunogenic, or other commercial biologic compositions, such as antibodies.

    Abstract translation: 提供了用于在细胞培养物中大规模生产蛋白质和/或多肽的改进方法。 根据本发明，该方法提供培养具有代谢转移的细胞。 使用这种方法或系统允许高水平的蛋白质或多肽产生并减少不期望的代谢废物如乳酸盐的积累。 根据本发明表达的蛋白质和多肽可有利地用于制备药物，免疫原性或其他商业生物组合物，例如抗体。

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