公开(公告)号：US20200248139A1

公开(公告)日：2020-08-06

申请号：US16637450

申请日：2018-08-08

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA

Inventor： Timothy D. O'Brien ,  Beth A. Lindborg ,  Amanda Vegoe ,  Jakub Tolar

IPC: C12N5/079 ,  A61K35/30

Abstract: This disclosure describes methods for organoid generation including, for example, for generation of a mid-brain organoid including an A9 neuron. Specifically, the methods comprising: introducing an input cell into a cell culture medium comprising hyaluronic acid, wherein the input cell comprises an embryonic stem cell, an induced pluripotent stem cell, or a neural progenitor cell; transferring the input cell to a cell culture device; and culturing the cell in the cell culture device for at least 7 days. This disclosure further describes methods for using the organoids.