公开(公告)号：US09784731B2

公开(公告)日：2017-10-10

申请号：US14280612

申请日：2014-05-18

申请人： Ramot at Tel-Aviv University Ltd.

发明人： Reuven Tirosh ,  Fernando Patolsky ,  Hagit Peretz-Soroka

IPC分类号： G01N33/50 ,  G01N33/84 ,  G06F19/12

CPC分类号： G01N33/5091 ,  G01N33/5005 ,  G01N33/5023 ,  G01N33/5038 ,  G01N33/5094 ,  G01N33/84 ,  G06F19/12

摘要： A method of measuring a metabolic activity (MA) of a cell is provided. The method comprising independently measuring in an extracellular environment of the cell, time-dependent acidification profiles due to secretion of: (i) non-volatile soluble metabolic products; (ii) non-volatile soluble metabolic products and volatile soluble metabolic products; and (iii) volatile soluble metabolic products; wherein at least one of the time dependent acidification profiles is indicative of the metabolic activity of the cell. Also provided are clinical methods which make use of the assay.

公开(公告)号：US11619634B2

公开(公告)日：2023-04-04

申请号：US16640392

申请日：2018-08-20

申请人： Savicell Diagnostic Ltd. ,  Ramot at Tel-Aviv University Ltd.

发明人： Giora Davidovits ,  Fernando Patolsky ,  David Eyal Davidovits ,  Irit Arbel ,  Shoval Tirman ,  Aviv Lutaty ,  Tali Scienmann ,  Reuven Tirosh ,  Hagit Peretz-Soroka

IPC分类号： G01N33/574 ,  G01N33/50 ,  A61K45/06

摘要： A method of diagnosing lung cancer in a subject-in-need thereof is provided. The method comprises: (a) providing a biological sample of the subject which comprises peripheral blood mononuclear cells (PBMCs); (b) in vitro contacting the PBMCs with a stimulant selected from the group consisting of the stimulants listed in Tables 3 and 4; and (c) measuring metabolic activity of the PBMCs having been contacted according to (b), wherein a statistically significant change in the metabolic activity of the PBMCs as compared to a control sample is indicative of lung cancer.

公开(公告)号：US20140255972A1

公开(公告)日：2014-09-11

申请号：US14280612

申请日：2014-05-18

申请人： Ramot at Tel-Aviv University Ltd.

发明人： Reuven TIROSH ,  Fernando Patolsky ,  Hagit Peretz-Soroka

IPC分类号： G01N33/50

CPC分类号： G01N33/5091 ,  G01N33/5005 ,  G01N33/5023 ,  G01N33/5038 ,  G01N33/5094 ,  G01N33/84 ,  G06F19/12

摘要： A method of measuring a metabolic activity (MA) of a cell is provided. The method comprising independently measuring in an extracellular environment of the cell, time-dependent acidification profiles due to secretion of:(i) non-volatile soluble metabolic products; (ii) non-volatile soluble metabolic products and volatile soluble metabolic products; and (iii) volatile soluble metabolic products; wherein at least one of the time dependent acidification profiles is indicative of the metabolic activity of the cell. Also provided are clinical methods which make use of the assay.

摘要翻译： 提供了测量细胞的代谢活性（MA）的方法。 该方法包括在细胞的细胞外环境中独立测量，由于分泌以下因素而导致的时间依赖性酸化曲线：（i）非挥发性可溶性代谢产物; （ii）不挥发性可溶性代谢产物和挥发性可溶性代谢产物; 和（iii）挥发性可溶性代谢产物; 其中至少一个时间依赖性酸化曲线指示细胞的代谢活性。 还提供了利用该测定法的临床方法。