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公开(公告)号：US06686454B1
公开(公告)日：2004-02-03
申请号：US09390280
申请日：1999-09-03
IPC分类号： C07K1600
CPC分类号： C07K7/64 , A61K39/00 , C07K16/14 , G01N33/9493 , Y10S435/975
摘要： This invention relates to the production of polyclonal and monoclonal antibodies to specific regions of cyclosporine (CSA) and/or CSA metabolites/derivatives. The reactivity of these polyclonal and monoclonal antibodies make them particularly useful for immunoassays for therapeutic drug monitoring (TDM). These immunoassays or TDM kits may include polyclonal or monoclonal antibodies to specific sites of CSA and/or CSA metabolites. These kits may also include various combinations of polyclonal antibodies, polyclonal and monoclonal antibodies or a panel of monoclonal antibodies. Cyclosporine or CSA metabolite conjugate immunogens are prepared for the immunization of a host animal to produce antibodies directed against specific regions of the CSA or CSA metabolite molecule. By determining the specific binding region of a particular antibody, immunoassays which are capable of distinguishing between the parent molecule, active metabolites, inactive metabolites and other structurally similar immunosuppressant compounds are developed. The use of divinyl sulfone (DVS) as the linker arm molecule for forming cyclosporine and cyclosporine metabolite protein conjugate immunogens is described.
摘要翻译： 本发明涉及针对环孢菌素（CSA）和/或CSA代谢物/衍生物的特定区域的多克隆和单克隆抗体的产生。 这些多克隆抗体和单克隆抗体的反应性使其在治疗药物监测（TDM）的免疫测定中特别有用。 这些免疫测定或TDM试剂盒可以包括针对CSA和/或CSA代谢物的特定位点的多克隆或单克隆抗体。 这些试剂盒还可以包括多克隆抗体，多克隆和单克隆抗体或一组单克隆抗体的各种组合。 制备环孢菌素或CSA代谢物缀合物免疫原用于免疫宿主动物以产生针对CSA或CSA代谢物分子的特定区域的抗体。 通过确定特定抗体的特异性结合区域，开发了能够区分亲本分子，活性代谢物，无活性代谢物和其它结构相似的免疫抑制剂化合物的免疫测定。 描述了使用二乙烯基砜（DVS）作为形成环孢菌素和环孢菌素代谢物蛋白质结合物免疫原的连接臂分子。
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公开(公告)号：US06713266B1
公开(公告)日：2004-03-30
申请号：US09650684
申请日：2000-08-30
IPC分类号： G01N3353
CPC分类号： G01N33/9493 , A61K39/00 , C07K7/64 , C07K16/14
摘要： This invention relates to the production of polyclonal and monoclonal antibodies to specific regions of cyclosporine (CSA) and/or CSA metabolites/derivatives. The reactivity of these polyclonal and monoclonal antibodies make them particularly useful for immunoassays for therapeutic drug monitoring (TDM). These immunoassays or TDM kits may include polyclonal or monoclonal antibodies to specific sites of CSA and/or CSA metabolites. These kits may also include various combinations of polyclonal antibodies, polyclonal and monoclonal antibodies or a panel of monoclonal antibodies. Cyclosporine or CSA metabolite conjugate immunogens are prepared for the immunization of a host animal to produce antibodies directed against specific regions of the CSA or CSA metabolite molecule. By determining the specific binding region of a particular antibody, immunoassays which are capable of distinguishing between the parent molecule, active metabolites, inactive metabolites and other structurally similar immunosuppressant compounds are developed. The use of divinyl sulfone (DVS) as the linker arm molecule for forming cyclosporine and cyclosporine metabolite protein conjugate immunogens is described.
摘要翻译： 本发明涉及针对环孢菌素（CSA）和/或CSA代谢物/衍生物的特定区域的多克隆和单克隆抗体的产生。 这些多克隆抗体和单克隆抗体的反应性使其在治疗药物监测（TDM）的免疫测定中特别有用。 这些免疫测定或TDM试剂盒可以包括针对CSA和/或CSA代谢物的特定位点的多克隆或单克隆抗体。 这些试剂盒还可以包括多克隆抗体，多克隆和单克隆抗体或一组单克隆抗体的各种组合。 制备环孢菌素或CSA代谢物缀合物免疫原用于免疫宿主动物以产生针对CSA或CSA代谢物分子的特定区域的抗体。 通过确定特定抗体的特异性结合区域，开发了能够区分亲本分子，活性代谢物，无活性代谢物和其它结构相似的免疫抑制剂化合物的免疫测定。 描述了使用二乙烯基砜（DVS）作为形成环孢菌素和环孢菌素代谢物蛋白质结合物免疫原的连接臂分子。
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公开(公告)号：US06709873B1
公开(公告)日：2004-03-23
申请号：US09638900
申请日：2000-08-15
IPC分类号： G01N3353
CPC分类号： C07K16/14
摘要： This invention relates to the production of polyclonal and monoclonal antibodies to specific sites of rapamycin (Sirolimus). The reactivity of these poly and monoclonal antibodies make them particularly useful for immunoassays for therapeutic drug monitoring (TDM). These immunoassays or TDM kits may include polyclonal or monoclonal antibodies to specific sites of rapamycin. These kits may also include various combinations of polyclonal antibodies, polyclonal and monoclonal antibodies or a panel of monoclonal antibodies. Rapamycin conjugate immunogens are prepared for the immunization of a host animal to produce antibodies directed against specific regions of the rapamycin molecule. By determining the specific binding region of particular antibody, immunoassays which are capable of distinguishing between the parent molecule, active metabolites, inactive metabolites and other structurally similar immunosuppressant compounds are developed. The use of divinyl sulfone (DVS) as the linker arm molecule for forming rapamycin-protein conjugate immunogens is described. DVS-linked rapamycin-protein conjugates were found to elicit antibodies with greater specificity to the rapamycin molecule than succinate linked conjugates.
摘要翻译： 本发明涉及针对雷帕霉素（西罗莫司）特定部位的多克隆和单克隆抗体的产生。 这些多抗和单克隆抗体的反应性使其对治疗药物监测（TDM）的免疫测定特别有用。 这些免疫测定或TDM试剂盒可以包括针对雷帕霉素的特定位点的多克隆或单克隆抗体。 这些试剂盒还可以包括多克隆抗体，多克隆和单克隆抗体或一组单克隆抗体的各种组合。 制备雷帕霉素结合物免疫原用于免疫宿主动物以产生针对雷帕霉素分子的特定区域的抗体。 通过确定特定抗体的特异性结合区域，开发了能够区分亲本分子，活性代谢物，无活性代谢物和其他结构类似的免疫抑制剂化合物的免疫测定。 描述了使用二乙烯基砜（DVS）作为形成雷帕霉素 - 蛋白质结合物免疫原的连接臂分子。 发现DVS连接的雷帕霉素 - 蛋白质缀合物比琥珀酸酯连接的缀合物引发对雷帕霉素分子具有更高特异性的抗体。
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4.发明授权Compositions for attenuating antibody- mediated xenograft rejection in human recipients 失效用于减弱人类受体中抗体介导的异种移植排斥的组合物
公开(公告)号：US5977079A
公开(公告)日：1999-11-02
申请号：US935637
申请日：1997-09-23
摘要： Antibody-mediated xenograft rejection is attenuated by (1) removing preformed antibodies to various identified carbohydrate xenoantigens from the recipient's circulation prior to transplantation by extracorporeal perfusion of the recipient's blood over a biocompatible solid support to which the xenoantigens are bound and/or (2) parenterally administering a xenoantibody-inhibiting amount of an identified xenoantigen to the recipient shortly before graft revascularization and thereafter.
摘要翻译： 抗体介导的异种移植排斥通过（1）在移植之前从接受者的循环中除去预先形成的各种鉴定的碳水化合物异种抗原的预防性抗体，其通过体外灌注接受者的血液与生物相容性固体支持物结合，所述生物相容性固体支持物与所述异种抗原结合，和/或（2） 在移植物血运重建之前不久向肠胃外给予异种抗体抑制量的鉴定的异种抗原素，此后。
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公开(公告)号：US6132723A
公开(公告)日：2000-10-17
申请号：US114886
申请日：1998-07-14
申请人： Andrew J. Malcolm
发明人： Andrew J. Malcolm
CPC分类号： A61K39/39 , A61K39/092 , A61K47/4833 , C07H3/06 , A61K2039/55583 , A61K2039/6037 , A61K2039/64
摘要： The invention provides immunogenic oligosaccharide compositions and methods of making and using them. In particular, the compositions comprise oligosaccharides covalently coupled to carrier protein, wherein the resultant conjugate has been shown to contain specific immunogenic epitopes and elicits a protectively immunogenic response.
摘要翻译： 本发明提供免疫原性寡糖组合物及其制备和使用方法。 特别地，组合物包含与载体蛋白共价偶联的寡糖，其中所得缀合物已显示含有特异性免疫原性表位并引发保护性免疫原性应答。
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6.发明授权Methods and compositions for attenuating antibody-mediated xenograft rejection in human recipients 失效用于减弱人类受体中抗体介导的异种移植排斥的方法和组合物公开(公告)号：US06607723B1
公开(公告)日：2003-08-19
申请号：US08458683
申请日：1995-06-02
IPC分类号： A61K34385
CPC分类号： A61K35/14 , A61K39/001 , A61M1/3679
摘要： Antibody-mediated xenograft rejection is attenuated by (1) removing preformed antibodies to various identified carbohydrate xenoantigens from the recipient's circulation prior to transplantation by extracorporeal perfusion of the recipient's blood over a biocompatible solid support to which the xenoantigens are bound and/or (2) parenterally administering a xenoantibody-inhibiting amount of an identified xenoantigen to the recipient shortly before graft revascularization and thereafter.
摘要翻译： 抗体介导的异种移植排斥通过（1）在移植之前从接受者的循环中除去预先形成的各种鉴定的碳水化合物异种抗原的预防性抗体，其通过体外灌注接受者的血液与生物相容性固体支持物结合，所述生物相容性固体支持物与所述异种抗原结合，和/或（2） 在移植物血运重建之前不久向肠胃外给予异种抗体抑制量的鉴定的异种抗原素，此后。
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7.发明授权Immunostimulating activity of Streptococcus pneumoniae serotype 8 oligosaccharides 失效肺炎链球菌血清型8寡糖的免疫刺激活性公开(公告)号：US06168796A
公开(公告)日：2001-01-02
申请号：US09225507
申请日：1999-01-06
申请人： Andrew J. Malcolm
发明人： Andrew J. Malcolm
IPC分类号： A61K3909
CPC分类号： C07H3/06 , A61K39/092 , A61K39/39 , A61K47/646 , A61K2039/55511 , A61K2039/55583 , A61K2039/6037 , A61K2039/64 , Y02A50/484
摘要： This invention provides compositions comprising an oligosaccharide of S. pneumoniae serotype 8 useful for stimulating an immune response to an antigen, methods of providing protective immunization against a bacterial pathogen using these compositions, methods of augmenting an immunogenic response to an antigen by administering these S. pneumoniae serotype 8 oligosaccharide compositions along with the antigen, and methods of making the immunostimulatory compositions described above.
摘要翻译： 本发明提供了包含可用于刺激对抗原的免疫应答的肺炎链球菌血清型8的寡糖的组合物，使用这些组合物对细菌病原体提供保护性免疫的方法，通过施用这些S增加对抗原的免疫原性应答的方法。 肺炎链球菌血清型8寡糖组合物以及抗原，以及制备上述免疫刺激组合物的方法。
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公开(公告)号：US5807553A
公开(公告)日：1998-09-15
申请号：US647602
申请日：1996-05-13
申请人： Andrew J. Malcolm
发明人： Andrew J. Malcolm
CPC分类号： A61K39/39 , A61K39/092 , A61K47/4833 , C07H3/06 , A61K2039/55583 , A61K2039/6037 , A61K2039/64
摘要： The invention provides immunogenic oligosaccharide compositions and methods of making and using them. In particular, the compositions comprise oligosaccharides covalently coupled to carrier protein, wherein the resultant conjugate has been shown to contain specific immunogenic epitopes and elicits a protectively immunogenic response.
摘要翻译： 本发明提供免疫原性寡糖组合物及其制备和使用方法。 特别地，组合物包含与载体蛋白共价偶联的寡糖，其中所得缀合物已显示含有特异性免疫原性表位并引发保护性免疫原性应答。
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9.发明授权Methods for attenuating antibody-mediated xenograft rejection in human recipients 失效减少人类受体抗体介导的异种移植排斥的方法
公开(公告)号：US5767093A
公开(公告)日：1998-06-16
申请号：US460094
申请日：1995-06-02
CPC分类号： A61K35/14 , A61K39/001 , A61M1/3679
摘要： Antibody-mediated xenograft rejection is attenuated by parenterally administering a xenoantibody-inhibiting amount of an identified carbohydrate xenoantigen to the recipient shortly before graft revascularization and thereafter.
摘要翻译： 抗体介导的异种移植物排斥通过在移植物血运重建之前不久向肠胃外给予异种抗体抑制量的鉴定的碳水化合物异种抗原来减毒。
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10.发明授权Immunostimulating activity of streptococcus pneumoniae serotype 8 oligosaccharides 失效肺炎链球菌血清型8寡糖的免疫刺激活性
公开(公告)号：US5916571A
公开(公告)日：1999-06-29
申请号：US787106
申请日：1997-01-22
申请人： Andrew J. Malcolm
发明人： Andrew J. Malcolm
CPC分类号： C07H3/06 , A61K39/092 , A61K39/39 , A61K47/4833 , A61K2039/55511 , A61K2039/55583 , A61K2039/6037 , A61K2039/64
摘要： The invention provides compositions comprising an oligosaccharide of S. pneumoniae serotype 8 useful for stimulating an immune response to an antigen, methods of providing protective immunization against a bacterial pathogen using these compositions, methods of augmenting an immunogenic response to an antigen by administering these S. pneumoniae serotype 8 oligosaccharide compositions along with the antigen, and methods of making the immunostimulatory compositions described above.
摘要翻译： 本发明提供了包含用于刺激对抗原的免疫应答的肺炎链球菌血清型8的寡糖的组合物，使用这些组合物提供对细菌病原体的保护性免疫的方法，通过施用这些S增加对抗原的免疫原性应答的方法。 肺炎链球菌血清型8寡糖组合物以及抗原，以及制备上述免疫刺激组合物的方法。
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