专利检索 ap:("Randolph V. Lewis" OR "Dong Chen" OR "Justin A. Jones" OR "Sreevidhya T. Krishnaji" OR "Paula F. Oliveira" OR "Gargi Bhattacharyya") AND inv:"Paula F. Oliveira" 第 1 页

1.

发明授权
Expression systems and associated methods 有权

公开(公告)号：US09714273B2

公开(公告)日：2017-07-25

申请号：US14879046

申请日：2015-10-08

申请人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya

发明人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya

IPC分类号： C12P21/02 , C07K14/435 , C12N15/70

CPC分类号： C07K14/43563 , C07K14/43518 , C12N15/70

摘要： A method of producing synthetic spider silk, including: transforming Escherichia coli with an expression vector; fermenting the transformed E. coli in a culture medium; inducing spider silk protein expression in the cultured E. coli; extracting the spider silk; and purifying the spider silk. Related vectors and genetically modified cells are also disclosed.

2.

发明申请
EXPRESSION SYSTEMS AND ASSOCIATED METHODS 有权
标题翻译：表达系统和相关方法

公开(公告)号：US20160102125A1

公开(公告)日：2016-04-14

申请号：US14879046

申请日：2015-10-08

申请人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya

发明人： Randolph V. Lewis , Dong Chen , Justin A. Jones , Sreevidhya T. Krishnaji , Paula F. Oliveira , Gargi Bhattacharyya

IPC分类号： C07K14/435 , C12N15/70

CPC分类号： C07K14/43563 , C07K14/43518 , C12N15/70

摘要： A method of producing synthetic spider silk, including: transforming Escherichia coli with an expression vector; fermenting the transformed E. coli in a culture medium; inducing spider silk protein expression in the cultured E. coli; extracting the spider silk; and purifying the spider silk. Related vectors and genetically modified cells are also disclosed.

摘要翻译： 一种生产合成蜘蛛丝的方法，包括：用表达载体转化大肠杆菌; 在培养基中发酵转化的大肠杆菌; 在培养的大肠杆菌中诱导蜘蛛丝蛋白表达; 提取蜘蛛丝; 并净化蜘蛛丝。还公开了相关载体和遗传修饰的细胞。

3.

发明申请
RECOMBINANT HAGFISH PROTEINS AND FIBERS 有权

公开(公告)号：US20220340622A1

公开(公告)日：2022-10-27

申请号：US17651020

申请日：2022-02-14

申请人： Justin A. JONES , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell

发明人： Justin A. JONES , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell

IPC分类号： C07K14/46 , D01F4/00

摘要： The present application provides fibers made from recombinant hagfish intermediate filament proteins. Methods of expressing and purifying the proteins, and methods of creating the fibers, are likewise disclosed. The method of purifying protein yields larger quantities of hagfish protein than have been previously achieved, and the fibers have the highest mechanical properties yet recorded for recombinant hagfish protein fibers.