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公开(公告)号：US20220340622A1
公开(公告)日：2022-10-27
申请号：US17651020
申请日：2022-02-14
Applicant: Justin A. JONES , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell
Inventor： Justin A. JONES , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell
Abstract: The present application provides fibers made from recombinant hagfish intermediate filament proteins. Methods of expressing and purifying the proteins, and methods of creating the fibers, are likewise disclosed. The method of purifying protein yields larger quantities of hagfish protein than have been previously achieved, and the fibers have the highest mechanical properties yet recorded for recombinant hagfish protein fibers.
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公开(公告)号：US20190010195A1
公开(公告)日：2019-01-10
申请号：US15417106
申请日：2017-01-26
Applicant: Randolph V. Lewis , Justin A. Jones , Thomas Ian Harris , Danielle Gaztambide , Breton A. Day
Inventor： Randolph V. Lewis , Justin A. Jones , Thomas Ian Harris , Danielle Gaztambide , Breton A. Day
IPC: C07K14/435 , A61L27/22 , A61L27/54
Abstract: Medical devices made with coatings made from recombinant spider silk proteins are disclosed. Methods to make the devices are also disclosed. Methods for adhering objects to one another using recombinant spider silk proteins are also disclosed.
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3.发明授权Phage-cosmid hybrid vector, open cos DNA fragments, their method of use, and process of production 失效噬菌体 - 粘粒杂合载体，开放式COS DNA片段，其使用方法和生产过程
公开(公告)号：US5695971A
公开(公告)日：1997-12-09
申请号：US418194
申请日：1995-04-07
Applicant: Yoichi Kadokami , Randolph V. Lewis
Inventor： Yoichi Kadokami , Randolph V. Lewis
CPC classification number: C12N15/73
Abstract: A hybrid vector produced from a linearized cosmid and arms from .lambda. phage vector DNA has been developed. The hybrid vector can be used to generate fragments which are useful as vectors in a helper phage-mediated transformation system, permitting large fragments of foreign DNA to be introduced into a host on an industrial scale.
Abstract translation: 已经开发了由线性化粘粒产生的杂交载体和来自λ噬菌体载体DNA的臂。 杂交载体可以用于产生可用作辅助噬菌体介导的转化系统中的载体的片段，允许外来DNA的大片段以工业规模引入宿主。
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公开(公告)号：US12234268B2
公开(公告)日：2025-02-25
申请号：US17651020
申请日：2022-02-14
Applicant: Justin A. Jones , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell
Inventor： Justin A. Jones , Randolph V. Lewis , Dong Chen , Paula F. Oliveira , Thomas Ian Harris , Brianne Eileen Bell
Abstract: The present application provides fibers made from recombinant hagfish intermediate filament proteins. Methods of expressing and purifying the proteins, and methods of creating the fibers, are likewise disclosed. The method of purifying protein yields larger quantities of hagfish protein than have been previously achieved, and the fibers have the highest mechanical properties yet recorded for recombinant hagfish protein fibers.
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公开(公告)号：US07723109B2
公开(公告)日：2010-05-25
申请号：US11926871
申请日：2007-10-29
Applicant: Randolph V. Lewis
Inventor： Randolph V. Lewis
CPC classification number: C07K14/43518 , C12N15/8257
Abstract: The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.
Abstract translation: 本发明涉及分子生物学和植物生物学领域。 具体地说，本发明涉及在植物中表达蜘蛛丝蛋白的方法以及从其中合成和提纯蜘蛛丝蛋白。
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Patent Agency Ranking
公开(公告)号：US20100092538A1
公开(公告)日：2010-04-15
申请号：US12561779
申请日：2009-09-17
Applicant: Omer Choresh , Randolph V. Lewis
Inventor： Omer Choresh , Randolph V. Lewis
CPC classification number: C07K14/43518 , A61K38/00 , A61L24/108
Abstract: Nucleic acids encoding spider glue proteins and methods of use thereof are disclosed.
Abstract translation: 公开了编码蜘蛛胶蛋白的核酸及其使用方法。
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7.发明授权Spider silk protein encoding nucleic acids, polypeptides, antibodies and methods of use thereof 有权蜘蛛丝蛋白编码核酸，多肽，抗体及其使用方法公开(公告)号：US07521228B2
公开(公告)日：2009-04-21
申请号：US10488056
申请日：2002-03-28
Applicant: Randolph V. Lewis , Cheryl Y. Hayashi , John E. Gatesy , Dagmara Motriuk
Inventor： Randolph V. Lewis , Cheryl Y. Hayashi , John E. Gatesy , Dagmara Motriuk
CPC classification number: C07K14/43518
Abstract: Spider silk protein encoding nucleic acids, polypeptides and antibodies immunologically specific therefore are disclosed. Methods of use thereof are also provided.
Abstract translation: 因此公开了编码免疫学特性的蜘蛛丝蛋白编码核酸，多肽和抗体。 还提供了其使用方法。
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公开(公告)号：US5728810A
公开(公告)日：1998-03-17
申请号：US425069
申请日：1995-04-19
Applicant: Randolph V. Lewis , Ming Xu , Michael B. Hinman
Inventor： Randolph V. Lewis , Ming Xu , Michael B. Hinman
IPC: C07K14/195 , C07K14/435 , C12N1/21 , A61K38/17
CPC classification number: C07K14/195 , C07K14/43518 , C07K2319/00
Abstract: The present invention is directed to isolated cDNA which codes for spider silk protein or a fragment or variant thereof, a replicable vector containing cDNA which codes for spider silk protein and which is capable of expressing spider silk protein, a transformed cell or microorganism containing cDNA which codes for spider silk protein or a fragment thereof which is capable of expressing spider silk protein and products, such as fibers, which may be manufactured utilizing the recombinant protein of the present invention.
Abstract translation: 本发明涉及编码蜘蛛丝蛋白或其片段或变体的分离的cDNA，包含编码蜘蛛丝蛋白并能够表达蜘蛛丝蛋白的cDNA的可复制载体，含有cDNA的转化细胞或微生物 能够表达蜘蛛丝蛋白的蜘蛛丝蛋白质或其片段的代码，以及可以使用本发明的重组蛋白质制造的产品，例如纤维。
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公开(公告)号：US20150292120A1
公开(公告)日：2015-10-15
申请号：US14681951
申请日：2015-04-08
Applicant: Randolph V. Lewis , Justin A. Jones , Cameron G. Copeland
Inventor： Randolph V. Lewis , Justin A. Jones , Cameron G. Copeland
CPC classification number: B29C48/022 , B29C48/0018 , B29C48/05 , B29C48/919 , B29K2089/00 , B29L2031/731 , D01D5/06 , D01D5/16 , D01F4/02
Abstract: Methods and apparatuses for preparing protein fibers (biofilaments) from recombinant biofilament proteins are disclosed. The methods are particularly useful for spinning fibers of spider silk or silkworm silk proteins from recombinant sources and may be used to spin such fibers for use in the manufacture of industrial and commercial products.
Abstract translation: 公开了从重组生物丝蛋白制备蛋白质纤维（生物丝）的方法和装置。 该方法特别适用于从重组来源纺丝蜘蛛丝或蚕丝蛋白的纤维，并可用于纺丝这种纤维用于制造工业和商业产品。
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公开(公告)号：US20150202651A1
公开(公告)日：2015-07-23
申请号：US14574235
申请日：2014-12-17
Applicant: Randolph V. Lewis , Justin A. Jones , Chauncey L. Tucker
Inventor： Randolph V. Lewis , Justin A. Jones , Chauncey L. Tucker
IPC: B05D3/06 , A61K8/64 , A61K35/646 , B01D29/00 , A61L27/22 , G02B1/10 , C08J5/18 , B29C35/08 , A61Q19/00
CPC classification number: B05D3/06 , A23V2002/00 , A61K8/0216 , A61K8/64 , A61K35/646 , A61K2800/10 , A61L27/227 , A61Q19/00 , B01D29/0093 , B29C35/08 , B29K2093/00 , B29L2007/00 , C08J5/18 , C08J2389/00 , G02B1/10 , Y10T428/31971 , Y10T442/20
Abstract: Methods for forming useful films using recombinant spider silk protein are discussed. In one embodiment, the method involves dissolving silk protein in a sufficient quantity to form a film suspended in a solvent. The solution is then mixed with a compound selected from water, acids, imidazole crosslinking agents, antibiotics, nanoparticles, surfactants and combinations thereof. Solubilisation of the protein in the solution is effected by microwave exposure. Once solubilized the microwave treated solution is poured onto a suitable substrate and dried in order to result in the final film, the protein may be plant, mammal or bacterially derived. The film has widespread use and can be combined with any one of a number of substrates, examples of which include: a cosmetic composition; a pharmaceutical or medical composition, drug delivery system, artificial cell, contact lens coating, sustained-release drug delivery system, artificial skin graft; food composition; automotive part; aeronautic component; computer or data storage device, building material, textile, filter material, membrane material, nanomaterial, electronic component and combinations thereof.
Abstract translation: 讨论了使用重组蜘蛛丝蛋白形成有用膜的方法。 在一个实施方案中，该方法包括将丝蛋白溶解在足够的量中以形成悬浮在溶剂中的膜。 然后将溶液与选自水，酸，咪唑交联剂，抗生素，纳米颗粒，表面活性剂及其组合的化合物混合。 溶液中蛋白质的溶解是通过微波暴露来实现的。 一旦溶解，将微波处理的溶液倒入合适的底物上并干燥以产生最终的膜，蛋白质可以是植物，哺乳动物或细菌来源的。 该膜具有广泛的用途，并且可以与多种基材中的任一种组合，其实例包括：化妆品组合物; 药物或药物组合物，药物递送系统，人造细胞，隐形眼镜涂层，缓释药物递送系统，人造皮肤移植物; 食物成分; 汽车零件; 航空部件; 计算机或数据存储装置，建筑材料，纺织品，过滤材料，膜材料，纳米材料，电子部件及其组合。
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