公开(公告)号：US5856122A

公开(公告)日：1999-01-05

申请号：US292968

申请日：1994-08-22

申请人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

发明人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

IPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/68 ,  C12P21/02

CPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/6893

摘要： The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.

公开(公告)号：US5977304A

公开(公告)日：1999-11-02

申请号：US467536

申请日：1995-06-06

申请人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

发明人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

IPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/68 ,  C07K1/00 ,  C07H21/04 ,  C12N1/20 ,  C12P21/06

CPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/6893

摘要： The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.

公开(公告)号：US5965385A

公开(公告)日：1999-10-12

申请号：US467974

申请日：1995-06-06

申请人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

发明人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

IPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/68 ,  C12P21/06 ,  C07H21/04 ,  C07K1/00 ,  C12N1/20

CPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/6893

摘要： The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.

公开(公告)号：US06168928A

公开(公告)日：2001-01-02

申请号：US09082514

申请日：1998-05-21

申请人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

发明人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

IPC分类号： C12N910

CPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/6893

摘要： The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.

摘要翻译： 结晶百日咳全毒素（PT）的三维结构已经通过X射线晶体学确定。 也已经确定了百日咳毒素与PT生物活性相关分子的复合物的晶体结构。 分析这些三维结构以鉴定适于修饰以改变PT的生物学性质的功能性氨基酸。 类似的程序可用于预测有助于全毒素毒性的氨基酸，产生免疫保护，百日咳毒素的遗传解毒类似物。

公开(公告)号：US6018022A

公开(公告)日：2000-01-25

申请号：US467976

申请日：1995-06-06

申请人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

发明人： Randy J. Read ,  Penelope E. Stein ,  Stephen A. Cockle ,  Raymond P. Oomen ,  Sheena Loosmore ,  Michel H. Klein ,  Glen D. Armstrong ,  Bart Hazes

IPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/68 ,  C07K1/00 ,  A61K39/10 ,  A61K39/108 ,  C12P21/06

CPC分类号： C07K14/235 ,  G01N33/566 ,  G01N33/6893

摘要： The three-dimensional structure of crystalline pertussis holotoxin (PT) has been determined by X-ray crystallography. Crystal structures have also been determined for complexes of pertussis toxin with molecules relevant to the biological activity of PT. These three-dimensional structures were analyzed to identify functional amino acids appropriate for modification to alter the biological properties of PT. Similar procedures may be used to predict amino acids which contribute to the toxicity of the holotoxin, to produce immunoprotective, genetically-detoxified analogs of pertussis toxin.

摘要翻译： 结晶百日咳全毒素（PT）的三维结构已经通过X射线晶体学确定。 也已经确定了百日咳毒素与PT生物活性相关分子的复合物的晶体结构。 分析这些三维结构以鉴定适于修饰以改变PT的生物学性质的功能性氨基酸。 类似的程序可用于预测有助于全毒素毒性的氨基酸，产生免疫保护，百日咳毒素的遗传解毒类似物。