公开(公告)号：US20130137117A1

公开(公告)日：2013-05-30

申请号：US13386426

申请日：2010-07-18

申请人： Raphael Levi ,  Ido Margalit ,  Orgad Laub ,  Yarden Dloomy

发明人： Raphael Levi ,  Ido Margalit ,  Orgad Laub ,  Yarden Dloomy

IPC分类号： G01N33/53

CPC分类号： G01N33/5308 ,  C12Q1/37 ,  G01N33/543 ,  G01N33/6803 ,  G01N2600/00 ,  G16B30/00

摘要： Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.

摘要翻译： 公开了制备分子印迹聚合物的方法及其用于检测样品中蛋白质和/或多肽的用途。 制备方法基于从可用数据库中选择蛋白质/多肽靶分子的氨基酸序列; 用至少一种裂解剂将该序列与硅裂解，产生具有已知组成的片段; 选择至少一个包含唯一表位的此类片段; 制备代表独特表位的合成肽; 并制备包含合成肽特异性结合位点的分子印迹聚合物。 为了检测样品中的靶蛋白，使用用于硅片切割的相同切割剂来切割靶蛋白以形成MIP特异性的特异性肽片段。

公开(公告)号：US20090110601A1

公开(公告)日：2009-04-30

申请号：US12318378

申请日：2008-12-29

申请人： Raphael Levi ,  Ido Margalit ,  Yarden Dloomy

发明人： Raphael Levi ,  Ido Margalit ,  Yarden Dloomy

IPC分类号： G01N33/566

CPC分类号： G01N33/5302 ,  G01N33/54386 ,  G01N33/558

摘要： Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.

摘要翻译： 用于快速和简单地测定液体样品中靶分子（包括小分子，多肽，蛋白质，细胞和感染性疾病）的装置，方法和试剂盒，其能够在高度选择性，高度选择性的流体样品中实时测量这些实体 敏感，操作简单，成本低，便携。 在至少一些实施例中，装置，方法和试剂盒还提供了在流动或横向流动装置中使用MIP。

公开(公告)号：US20130102063A1

公开(公告)日：2013-04-25

申请号：US13341943

申请日：2011-12-31

申请人： Rafael LEVI ,  Ido Margalit ,  Yarden Dloomy

发明人： Rafael LEVI ,  Ido Margalit ,  Yarden Dloomy

IPC分类号： G01N33/53

CPC分类号： G01N33/5302 ,  G01N33/54386 ,  G01N33/558

摘要： Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.

摘要翻译： 用于快速和简单地测定液体样品中靶分子（包括小分子，多肽，蛋白质，细胞和感染性疾病）的装置，方法和试剂盒，其能够在高度选择性，高度选择性的流体样品中实时测量这些实体 敏感，操作简单，成本低，便携。 在至少一些实施例中，装置，方法和试剂盒还提供了在流动或横向流动装置中使用MIP。