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    METHOD FOR PREPARING PROTEIN IMPRINTED POLYMERS AND USE THEREOF
    1.
    发明申请
    METHOD FOR PREPARING PROTEIN IMPRINTED POLYMERS AND USE THEREOF 审中-公开
    制备蛋白质印迹聚合物的方法及其用途

    公开(公告)号：US20130137117A1

    公开(公告)日：2013-05-30

    申请号：US13386426

    申请日：2010-07-18

    申请人： Raphael Levi ,  Ido Margalit ,  Orgad Laub ,  Yarden Dloomy

    发明人： Raphael Levi ,  Ido Margalit ,  Orgad Laub ,  Yarden Dloomy

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5308 ,  C12Q1/37 ,  G01N33/543 ,  G01N33/6803 ,  G01N2600/00 ,  G16B30/00

    摘要： Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.

    摘要翻译： 公开了制备分子印迹聚合物的方法及其用于检测样品中蛋白质和/或多肽的用途。 制备方法基于从可用数据库中选择蛋白质/多肽靶分子的氨基酸序列; 用至少一种裂解剂将该序列与硅裂解，产生具有已知组成的片段; 选择至少一个包含唯一表位的此类片段; 制备代表独特表位的合成肽; 并制备包含合成肽特异性结合位点的分子印迹聚合物。 为了检测样品中的靶蛋白，使用用于硅片切割的相同切割剂来切割靶蛋白以形成MIP特异性的特异性肽片段。

    Intravenous immunoglobulin composition
    3.
    发明授权
    Intravenous immunoglobulin composition 有权
    静脉内免疫球蛋白组成

    公开(公告)号：US08354249B2

    公开(公告)日：2013-01-15

    申请号：US11201282

    申请日：2005-08-11

    申请人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    发明人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    IPC分类号： C12P21/04

    CPC分类号： C07K16/081 ,  A61K38/00 ,  A61K39/39591 ,  A61K2039/505 ,  C07K2319/00

    摘要： A method for preparing a concentrated, immunoglobulin composition for treating subjects vaccinated against or infected with a pathogenic microorganism, comprising: (a) selecting a population of individuals previously vaccinated against one or more antigens associated with the pathogenic microorganism; (b) determining the level of specific antibodies immunoreactive with the pathogenic microorganism in a blood or blood component of the individuals to identify very high titre individuals having a very high titre of the specific antibodies; (c) combining blood or blood components comprising immunoglobulins from the very high titre individuals; and (d) purifying and/or concentrating the product of step (c), thereby obtaining a concentrated immunoglobulin composition. Also disclosed is a concentrated immunoglobulin composition comprising specific antibodies immunoreactive with a pathogenic microorganism, characterized in that the titre of specific antibodies of the composition is at least 5 times higher than the average titre of specific antibodies of a population of individuals previously vaccinated against one or more antigens associated with the pathogenic microorganism. The composition has a relatively high protein concentration and a low percentage of protein aggregates, and is therefore suitable for both iv and im administration. In a preferred embodiment, the pathogenic microorganism is smallpox virus or vaccinia virus.

    摘要翻译： 一种制备用于治疗接种疫苗或感染病原微生物的受试者的浓缩免疫球蛋白组合物的方法，包括：（a）选择先前针对与病原微生物相关的一种或多种抗原接种的个体群体; （b）确定在个体的血液或血液成分中与病原微生物免疫反应的特异性抗体的水平，以鉴定具有非常高的特异性抗体滴度的极高滴度的个体; （c）从非常高的滴度个体组合包含免疫球蛋白的血液或血液成分; 和（d）纯化和/或浓缩步骤（c）的产物，由此获得浓缩的免疫球蛋白组合物。 还公开了包含与病原微生物免疫反应性的特异性抗体的浓缩免疫球蛋白组合物，其特征在于组合物的特异性抗体滴度比先前接种于一种或多种疫苗的个体群体的特异性抗体的平均滴度高至少5倍 更多与病原微生物相关的抗原。 该组合物具有相对高的蛋白质浓度和低百分比的蛋白质聚集体，因此适用于iv和im施用。 在优选的实施方案中，致病微生物是天花病毒或痘苗病毒。

    Subcutaneous administration of anti-hepatitis B antibodies
    4.
    发明授权
    Subcutaneous administration of anti-hepatitis B antibodies 有权
    皮下给予抗乙型肝炎抗体

    公开(公告)号：US08795671B2

    公开(公告)日：2014-08-05

    申请号：US12630433

    申请日：2009-12-03

    申请人： Orgad Laub ,  Nadav Orr

    发明人： Orgad Laub ,  Nadav Orr

    IPC分类号： A61K39/395 ,  A61P31/12

    CPC分类号： C07K16/082 ,  A61K2039/505 ,  A61K2039/54 ,  C07K16/06

    摘要： The present application relates to an immunoglobulin preparation comprising anti-hepatitis B antibodies for subcutaneous administration and to uses thereof, including methods for treating, preventing and/or reducing a disease, disorder or condition associated with hepatitis B virus.

    摘要翻译： 本申请涉及包含用于皮下给药的抗乙型肝炎抗体及其用途的免疫球蛋白制品，包括用于治疗，预防和/或减少与乙型肝炎病毒相关的疾病，病症或病状的方法。

    Intravenous immunoglobulin composition
    5.
    发明申请
    Intravenous immunoglobulin composition 有权
    静脉内免疫球蛋白组成

    公开(公告)号：US20070037170A1

    公开(公告)日：2007-02-15

    申请号：US11201282

    申请日：2005-08-11

    申请人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    发明人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： C07K16/081 ,  A61K38/00 ,  A61K39/39591 ,  A61K2039/505 ,  C07K2319/00

    摘要： A method for preparing a concentrated, immunoglobulin composition for treating subjects vaccinated against or infected with a pathogenic microorganism, comprising: (a) selecting a population of individuals previously vaccinated against one or more antigens associated with the pathogenic microorganism; (b) determining the level of specific antibodies immunoreactive with the pathogenic microorganism in a blood or blood component of the individuals to identify very high titre individuals having a very high titre of the specific antibodies; (c) combining blood or blood components comprising immunoglobulins from the very high titre individuals; and (d) purifying and/or concentrating the product of step (c), thereby obtaining a concentrated immunoglobulin composition. Also disclosed is a concentrated immunoglobulin composition comprising specific antibodies immunoreactive with a pathogenic microorganism, characterized in that the titre of specific antibodies of the composition is at least 5 times higher than the average titre of specific antibodies of a population of individuals previously vaccinated against one or more antigens associated with the pathogenic microorganism. The composition has a relatively high protein concentration and a low percentage of protein aggregates, and is therefore suitable for both iv and im administration. In a preferred embodiment, the pathogenic microorganism is smallpox virus or vaccinia virus.

    摘要翻译： 一种制备用于治疗接种疫苗或感染病原微生物的受试者的浓缩免疫球蛋白组合物的方法，包括：（a）选择先前针对与病原微生物相关的一种或多种抗原接种的个体群体; （b）确定在个体的血液或血液成分中与病原微生物免疫反应的特异性抗体的水平，以鉴定具有非常高的特异性抗体滴度的极高滴度的个体; （c）从非常高的滴度个体组合包含免疫球蛋白的血液或血液成分; 和（d）纯化和/或浓缩步骤（c）的产物，由此获得浓缩的免疫球蛋白组合物。 还公开了包含与病原微生物免疫反应性的特异性抗体的浓缩免疫球蛋白组合物，其特征在于组合物的特异性抗体滴度比先前接种于一种或多种疫苗的个体群体的特异性抗体的平均滴度高至少5倍 更多与病原微生物相关的抗原。 该组合物具有相对高的蛋白质浓度和低百分比的蛋白质聚集体，因此适用于iv和im施用。 在优选的实施方案中，致病微生物是天花病毒或痘苗病毒。

    Intravenous immunoglobulin composition
    7.
    发明授权
    Intravenous immunoglobulin composition 有权
    静脉内免疫球蛋白组成

    公开(公告)号：US09365635B2

    公开(公告)日：2016-06-14

    申请号：US13619011

    申请日：2012-09-14

    申请人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    发明人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    IPC分类号： A61K38/00 ,  C07K16/08 ,  A61K39/395 ,  A61K39/00

    CPC分类号： C07K16/081 ,  A61K38/00 ,  A61K39/39591 ,  A61K2039/505 ,  C07K2319/00

    摘要： A concentrated, immunoglobulin composition for treating subjects vaccinated against or infected with a pathogenic microorganism, is made by (a) selecting a population of individuals previously vaccinated against antigens associated with the pathogenic microorganism; (b) identifying very high titer individuals by determining the level of specific antibodies immunoreactive with the pathogenic microorganism in the blood of the individuals; (c) combining blood from the very high titer individuals; and (d) purifying and/or concentrating the product of step (c). A concentrated immunoglobulin composition can include specific antibodies immunoreactive with a pathogenic microorganism, wherein the titer of specific antibodies is at least 5 times higher than the average titer of specific antibodies of a population of individuals previously vaccinated against antigens associated with the pathogenic microorganism. The composition has a relatively high protein concentration and a low percentage of protein aggregates. The pathogenic microorganism is preferably smallpox virus or vaccinia virus.

    摘要翻译： 用于治疗接种疫苗或感染致病微生物的受试者的浓缩的免疫球蛋白组合物是通过（a）选择先前针对与病原微生物相关的抗原进行接种的个体群体; （b）通过确定与个体血液中的病原微生物免疫反应的特异性抗体的水平来鉴定非常高的滴度个体; （c）结合来自极高滴度个体的血液; 和（d）纯化和/或浓缩步骤（c）的产物。 浓缩的免疫球蛋白组合物可以包括与病原微生物免疫反应的特异性抗体，其中特异性抗体的滴度比先前接种抗原与病原微生物相关抗原的个体群体的特异性抗体的平均滴度高至少5倍。 该组合物具有相对高的蛋白质浓度和低百分比的蛋白质聚集体。 病原微生物优选为天花病毒或痘苗病毒。

    INTRAVENOUS IMMUNOGLOBULIN COMPOSITION
    8.
    发明申请
    INTRAVENOUS IMMUNOGLOBULIN COMPOSITION 有权
    内脏免疫组织化学

    公开(公告)号：US20130011388A1

    公开(公告)日：2013-01-10

    申请号：US13619011

    申请日：2012-09-14

    申请人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    发明人： Israel Nur ,  Liliana Bar ,  Orgad Laub

    IPC分类号： A61K39/395 ,  C07K16/08 ,  A61P31/20 ,  C07K16/00

    CPC分类号： C07K16/081 ,  A61K38/00 ,  A61K39/39591 ,  A61K2039/505 ,  C07K2319/00

    摘要： A concentrated, immunoglobulin composition for treating subjects vaccinated against or infected with a pathogenic microorganism, is made by (a) selecting a population of individuals previously vaccinated against antigens associated with the pathogenic microorganism; (b) identifying very high titre individuals by determining the level of specific antibodies immunoreactive with the pathogenic microorganism in the blood of the individuals; (c) combining blood from the very high titre individuals; and (d) purifying and/or concentrating the product of step (c). A concentrated immunoglobulin composition can include specific antibodies immunoreactive with a pathogenic microorganism, wherein the titre of specific antibodies is at least 5 times higher than the average titre of specific antibodies of a population of individuals previously vaccinated against antigens associated with the pathogenic microorganism. The composition has a relatively high protein concentration and a low percentage of protein aggregates. The pathogenic microorganism is preferably smallpox virus or vaccinia virus.

    摘要翻译： 用于治疗接种疫苗或感染致病微生物的受试者的浓缩的免疫球蛋白组合物是通过（a）选择先前针对与病原微生物相关的抗原进行接种的个体群体; （b）通过确定与个体血液中的病原微生物免疫反应的特异性抗体的水平来鉴定非常高的滴度个体; （c）结合极高滴度个体的血液; 和（d）纯化和/或浓缩步骤（c）的产物。 浓缩的免疫球蛋白组合物可以包括与病原微生物免疫反应的特异性抗体，其中特异性抗体的滴度比先前接种抗原与病原微生物相关抗原的个体群体的特异性抗体的平均滴度高至少5倍。 该组合物具有相对高的蛋白质浓度和低百分比的蛋白质聚集体。 病原微生物优选为天花病毒或痘苗病毒。