公开(公告)号：US20050002954A1

公开(公告)日：2005-01-06

申请号：US10846548

申请日：2004-05-17

申请人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

发明人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

IPC分类号： A61K20060101 ,  A61K39/112 ,  A61K39/145 ,  A61P27/16 ,  A61P31/16 ,  C07K7/06 ,  C07K7/08 ,  A61K39/12

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/385 ,  A61K2039/543 ,  A61K2039/58 ,  A61K2039/6068 ,  C12N2760/16134

摘要： A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.

摘要翻译： 用于鼻内给药的基于人的基于合成肽的流感疫苗包含含有至少四种与人类细胞反应的流感病毒表位的鞭毛的混合物，每种表达单独在沙门氏菌鞭毛蛋白中表达，所述流感病毒表位选自：（i ）一个B细胞血凝素（HA）表位; （ii）可以结合许多HLA分子的一种T辅助血凝素（HA）或核蛋白（NP）表位; 和（iii）限于不同人群中最流行的HLA分子的至少两种细胞毒性淋巴细胞（CTL）核蛋白（NP）或基质蛋白（M）表位。

公开(公告)号：US06740325B1

公开(公告)日：2004-05-25

申请号：US09856920

申请日：2001-07-30

申请人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

发明人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

IPC分类号： A61K39145

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/385 ,  A61K2039/543 ,  A61K2039/58 ,  A61K2039/6068 ,  C12N2760/16134

摘要： A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.

摘要翻译： 用于鼻内给药的基于人的基于合成肽的流感疫苗包含含有至少四种与人类细胞反应的流感病毒表位的鞭毛的混合物，每种表达单独在沙门氏菌鞭毛蛋白中表达，所述流感病毒表位选自：（i ）一个B细胞血凝素（HA）表位; （ii）可以结合许多HLA分子的一种T辅助血凝素（HA）或核蛋白（NP）表位; 和（iii）限于不同人群中最流行的HLA分子的至少两种细胞毒性淋巴细胞（CTL）核蛋白（NP）或基质蛋白（M）表位。

公开(公告)号：US20130137117A1

公开(公告)日：2013-05-30

申请号：US13386426

申请日：2010-07-18

申请人： Raphael Levi ,  Ido Margalit ,  Orgad Laub ,  Yarden Dloomy

发明人： Raphael Levi ,  Ido Margalit ,  Orgad Laub ,  Yarden Dloomy

IPC分类号： G01N33/53

CPC分类号： G01N33/5308 ,  C12Q1/37 ,  G01N33/543 ,  G01N33/6803 ,  G01N2600/00 ,  G16B30/00

摘要： Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.

摘要翻译： 公开了制备分子印迹聚合物的方法及其用于检测样品中蛋白质和/或多肽的用途。 制备方法基于从可用数据库中选择蛋白质/多肽靶分子的氨基酸序列; 用至少一种裂解剂将该序列与硅裂解，产生具有已知组成的片段; 选择至少一个包含唯一表位的此类片段; 制备代表独特表位的合成肽; 并制备包含合成肽特异性结合位点的分子印迹聚合物。 为了检测样品中的靶蛋白，使用用于硅片切割的相同切割剂来切割靶蛋白以形成MIP特异性的特异性肽片段。

公开(公告)号：US20070122424A1

公开(公告)日：2007-05-31

申请号：US11672429

申请日：2007-02-07

申请人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

发明人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

IPC分类号： A61K39/145 ,  A61K39/12

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/385 ,  A61K2039/543 ,  A61K2039/58 ,  A61K2039/6068 ,  C12N2760/16134

摘要： A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.

摘要翻译： 用于鼻内给药的基于人的基于合成肽的流感疫苗包含含有至少四种与人类细胞反应的流感病毒表位的鞭毛的混合物，每种表达单独在沙门氏菌鞭毛蛋白中表达，所述流感病毒表位选自：（i ）一个B细胞血凝素（HA）表位; （ii）可以结合许多HLA分子的一种T辅助血凝素（HA）或核蛋白（NP）表位; 和（iii）限于不同人群中最流行的HLA分子的至少两种细胞毒性淋巴细胞（CTL）核蛋白（NP）或基质蛋白（M）表位。

公开(公告)号：US07192595B2

公开(公告)日：2007-03-20

申请号：US10846548

申请日：2004-05-17

申请人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

发明人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

IPC分类号： A61K39/145

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/385 ,  A61K2039/543 ,  A61K2039/58 ,  A61K2039/6068 ,  C12N2760/16134

摘要： A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.

摘要翻译： 用于鼻内给药的基于人的基于合成肽的流感疫苗包含含有至少四种与人类细胞反应的流感病毒表位的鞭毛的混合物，每种表达单独在沙门氏菌鞭毛蛋白中表达，所述流感病毒表位选自：（i ）一个B细胞血凝素（HA）表位; （ii）可以结合许多HLA分子的一种T辅助血凝素（HA）或核蛋白（NP）表位; 和（iii）限于不同人群中最流行的HLA分子的至少两种细胞毒性淋巴细胞（CTL）核蛋白（NP）或基质蛋白（M）表位。

公开(公告)号：US07021122B1

公开(公告)日：2006-04-04

申请号：US09646348

申请日：1999-03-18

申请人： Yossef Rosemberg ,  Falk Fish ,  Raphael Levi

发明人： Yossef Rosemberg ,  Falk Fish ,  Raphael Levi

IPC分类号： G01N11/00

CPC分类号： G01N33/491 ,  G01N11/04 ,  G01N2011/0066

摘要： A sensor device (10) and a method for measuring a viscosity of a sample of a liquid. The sensor features a chamber (12) for receiving the sample of the liquid, two electrodes (18) disposed either on the surfaces of the chamber, but possibly isolated from contacting the sample, or electrodes located externally to the chamber, and a capacitance measuring circuit (25). The sensor is used by connecting to a measurer for determining a capacitance on the electrodes, such that the capacitance directly reflects a volume occupied by the sample of the liquid. Preferably, the liquid is blood and the capacitance is used to determine the clotting time of the blood. Also preferably, a cover for the chamber is provided with at least one aperture, which more preferably is a mesh. Alternatively, an electrical property such as the amplitude of current passed through the sample is used to determine clotting time. Also preferably, the time period required for the maximum capacitance to be reached could be measured. Alternatively, the capacitance could be measured after a fixed time period (34) had elapsed. Also alternatively, the time period required for the maximum current to be reached could be measured, or the amount of current could be measured after a fixed time period had elapsed. In preferred embodiments of the present invention, a kit for measuring the clotting time of blood is provided. In other preferred embodiments of the present invention, methods are provided for using the kit and device of the present invention.

摘要翻译： 传感器装置（10）和用于测量液体样品的粘度的方法。 传感器具有用于接收液体样品的腔室（12），设置在腔室表面上但可能与接触样品或位于腔室外部的电极隔离的两个电极（18）和电容测量 电路（25）。 传感器通过连接到用于确定电极上的电容的测量器来使用，使得电容直接反映由液体样品占据的体积。 优选地，液体是血液，并且电容用于确定血液的凝血时间。 还优选地，用于腔室的盖设置有至少一个孔，更优选地是网。 或者，使用诸如通过样品的电流的振幅的电特性来确定凝固时间。 还优选地，可以测量达到最大电容所需的时间。 或者，可以在经过固定时间段（34）之后测量电容。 或者，可以测量达到最大电流所需的时间段，或者在固定时间段过去之后可以测量电流量。 在本发明的优选实施方案中，提供了用于测量血液凝血时间的试剂盒。 在本发明的其它优选实施方案中，提供了使用本发明的试剂盒和装置的方法。

公开(公告)号：US07514086B2

公开(公告)日：2009-04-07

申请号：US11672429

申请日：2007-02-07

申请人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

发明人： Ruth Arnon ,  Tamar Ben-Yedidia ,  Raphael Levi

IPC分类号： A61K39/145

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K39/385 ,  A61K2039/543 ,  A61K2039/58 ,  A61K2039/6068 ,  C12N2760/16134

摘要： A human synthetic peptide-based influenza vaccine for intranasal administration comprises a mixture of flagella containing at least four epitopes of influenza virus reactive with human cells, each expressed individually in Salmonella flagellin, said influenza virus epitopes being selected from the group consisting of: (i) one B-cell hemagglutinin (HA) epitope; (ii) one T-helper hemagglutinin (HA) or nucleo-protein (NP) epitope that can bind to many HLA molecules; and (iii) at least two cytotoxic lymphocyte (CTL) nucleoprotein (NP) or matrix protein (M) epitopes that are restricted to the most prevalent HLA molecules in different human populations.

摘要翻译： 用于鼻内给药的基于人的基于合成肽的流感疫苗包含含有至少四种与人类细胞反应的流感病毒表位的鞭毛的混合物，每种表达单独在沙门氏菌鞭毛蛋白中表达，所述流感病毒表位选自：（i ）一个B细胞血凝素（HA）表位; （ii）可以结合许多HLA分子的一种T辅助血凝素（HA）或核蛋白（NP）表位; 和（iii）限于不同人群中最流行的HLA分子的至少两种细胞毒性淋巴细胞（CTL）核蛋白（NP）或基质蛋白（M）表位。

公开(公告)号：US20090110601A1

公开(公告)日：2009-04-30

申请号：US12318378

申请日：2008-12-29

申请人： Raphael Levi ,  Ido Margalit ,  Yarden Dloomy

发明人： Raphael Levi ,  Ido Margalit ,  Yarden Dloomy

IPC分类号： G01N33/566

CPC分类号： G01N33/5302 ,  G01N33/54386 ,  G01N33/558

摘要： Devices, methods and kits for rapid and simple determination of target molecules, including small molecules, polypeptides, proteins, cells and infectious disease agents in liquid samples that are capable of real-time measurement of these entities in fluid samples that are highly selective, highly sensitive, simple to operate, low cost, and portable. The devices, methods and kits also provide, in at least some embodiments, the use of MIPs in a flow through or lateral flow device.

摘要翻译： 用于快速和简单地测定液体样品中靶分子（包括小分子，多肽，蛋白质，细胞和感染性疾病）的装置，方法和试剂盒，其能够在高度选择性，高度选择性的流体样品中实时测量这些实体 敏感，操作简单，成本低，便携。 在至少一些实施例中，装置，方法和试剂盒还提供了在流动或横向流动装置中使用MIP。