公开(公告)号：US20230296559A1

公开(公告)日：2023-09-21

申请号：US18185624

申请日：2023-03-17

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Nisha PALACKAL ,  Kun LU ,  Gangadhar DHULIPALA ,  Erica PYLES

IPC分类号： G01N27/447

CPC分类号： G01N27/44795 ,  G01N27/44726

摘要： A method of quantifying charge variants within an analyte may include introducing a sample buffer comprising the analyte into a capillary, separating charge variants within the sample buffer along an isoelectric gradient, incubating the capillary in a detection antibody, quantifying a relative abundance of a charge variant based on a signal that corresponds to the detection antibody. The method may further include generating an electropherogram, wherein the electropherogram includes a plot of a strength of a signal generated by a reporter molecule versus an isoelectric point along the isoelectric gradient where the signal was detected.

公开(公告)号：US20200369720A1

公开(公告)日：2020-11-26

申请号：US16882453

申请日：2020-05-23

申请人： Regeneron Pharmaceuticals, Inc.

发明人： Sophia Liu ,  Jennifer NGUYEN ,  Michael ROSCONI ,  Erica PYLES

IPC分类号： C07K1/28 ,  C07K1/18 ,  C07K7/06

摘要： The present invention provides methods and systems for analyzing the biophysical characteristics of peptides or proteins, such as antibodies, based on enzymatic digestion-assisted imaged capillary electrophoresis (DiCE) to characterize the domain-specific charge variants, preferably within a bispecific antibody. The methods and systems include treating the protein with digestion enzymes to generate components of the protein, reducing or denaturing the components, and separating the components based on their isoelectric points.