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公开(公告)号：US20230002445A1
公开(公告)日：2023-01-05
申请号：US17853807
申请日：2022-06-29
发明人： Tao Xing , Yuetian Yan , Shunhai Wang
摘要： The present invention generally pertains to methods of characterizing charge variants of a protein of interest. In particular, the present invention pertains to the use of desalting size exclusion chromatography-reduced peptide mapping mass spectrometry to identify charge variants separated by capillary isoelectric focusing.
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公开(公告)号：US11318470B2
公开(公告)日：2022-05-03
申请号：US16349700
申请日：2017-11-17
摘要： Microfluidic devices and nanobiosensors comprising a magnetic nanoparticle attached to a reporter molecule via a release unit for microfluidic-based detection of a target analyte in a biological sample. The nanobiosensors can be magnetically manipulated or guided through the microfluidics channels for incubation with the biological sample, concentration of the nanobiosensors, and detection of target analytes, without having to pump the entire initial sample through a microfluidic channel of the microfluidic device. The magnetic nanoparticles are separated from the reporter molecules before detection and can be re-used.
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公开(公告)号：US20200369720A1
公开(公告)日：2020-11-26
申请号：US16882453
申请日：2020-05-23
发明人： Sophia Liu , Jennifer NGUYEN , Michael ROSCONI , Erica PYLES
摘要： The present invention provides methods and systems for analyzing the biophysical characteristics of peptides or proteins, such as antibodies, based on enzymatic digestion-assisted imaged capillary electrophoresis (DiCE) to characterize the domain-specific charge variants, preferably within a bispecific antibody. The methods and systems include treating the protein with digestion enzymes to generate components of the protein, reducing or denaturing the components, and separating the components based on their isoelectric points.
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公开(公告)号：US10578580B2
公开(公告)日：2020-03-03
申请号：US15741883
申请日：2017-09-22
发明人： Norman Dovichi , Guijie Zhu , Liangliang Sun
IPC分类号： G01N27/447 , G01N30/72 , G01N30/00 , C07K1/28
摘要： The invention provides a capillary isoelectric focusing (cIEF) system based on a sandwich injection method for automated chemical mobilization. This system was coupled with an electrokinetically pumped nanoelectrospray interface to a mass spectrometer. The nanoelectrospray emitter employed an acidic sheath electrolyte. To realize automated focusing and mobilization, a plug of ammonium hydroxide was first injected into the capillary, followed by a section of mixed sample and ampholyte. As focusing progressed, the NH3H2O section was titrated to lower pH buffer by the acidic sheath buffer. Chemical mobilization started automatically once the ammonium hydroxide was consumed by the acidic sheath flow electrolyte.
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公开(公告)号：US20190004010A1
公开(公告)日：2019-01-03
申请号：US15741883
申请日：2017-09-22
发明人： Norman DOVICHI , Guijie ZHU , Liangliang SUN
IPC分类号： G01N27/447 , G01N30/72 , C07K1/28
摘要： The invention provides a capillary isoelectric focusing (cIEF) system based on a sandwich injection method for automated chemical mobilization. This system was coupled with an electrokinetically pumped nanoelectrospray interface to a mass spectrometer. The nanoelectrospray emitter employed an acidic sheath electrolyte. To realize automated focusing and mobilization, a plug of ammonium hydroxide was first injected into the capillary, followed by a section of mixed sample and ampholyte. As focusing progressed, the NH3H2O section was titrated to lower pH buffer by the acidic sheath buffer. Chemical mobilization started automatically once the ammonium hydroxide was consumed by the acidic sheath flow electrolyte.
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公开(公告)号：US09766207B2
公开(公告)日：2017-09-19
申请号：US14468730
申请日：2014-08-26
发明人： Aran Paulus , Camille Diges , Roumen Bogoev , Inbal Zafir-Lavie , Sricharan Bandhakavi , Annett Hahn-Windgassen , Anton Posch , Elad Brod , Uri Sivan
IPC分类号： G01N27/447 , C07K1/28 , C07K1/36 , G01N33/53
CPC分类号： G01N27/44795 , C07K1/28 , C07K1/36 , G01N33/5302
摘要： Methods and devices for purifying, detecting, and collecting analytes fractionated based on pI, separating analytes via electrophoresis and pI, and purifying a target molecule using pI focusing and subsequent crystallization are provided.
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7.发明授权公开(公告)号：US09028664B2
公开(公告)日：2015-05-12
申请号：US13390352
申请日：2010-08-18
IPC分类号： G01N27/447 , B01D57/02 , B01D61/44 , C07K1/28 , G01N21/80
CPC分类号： G01N27/44795 , B01D57/02 , B01D61/445 , C07K1/28 , G01N21/80 , G01N27/44747 , G01N27/44773
摘要： A device for isoelectric focusing. The device comprises a focusing container configured to contain an electrolyte solution and having a longitudinal axis and at least one electrolysis unit mounted in a close proximity to the longitudinal axis. Each electrolysis unit injects an ion flow into the focusing container so as to create a pH gradient having a plurality of steps in the electrolyte solution, along the longitudinal axis. Each step has a substantially uniform pH level and the pH gradient is defined by at least one pH ramp between every two sequential steps of the plurality of steps.
摘要翻译： 等电聚焦装置。 该装置包括配置成容纳电解质溶液并具有纵向轴线的至少一个电解单元的聚焦容器，并且至少一个电解单元安装在靠近纵向轴线的位置。 每个电解单元将离子流注入到聚焦容器中，以沿着纵向轴线产生在电解液中具有多个步骤的pH梯度。 每个步骤具有基本上均匀的pH水平，并且pH梯度由多个步骤的每两个连续步骤之间的至少一个pH斜坡限定。
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公开(公告)号：US20100173064A1
公开(公告)日：2010-07-08
申请号：US12458036
申请日：2009-06-30
IPC分类号： A23J1/14 , A23L2/66 , C07K1/28 , C07K14/415
CPC分类号： C07K14/415
摘要： A novel canola protein isolate consisting predominantly of 2S canola protein and having equal to better solubility properties and improved clarity properties in aqueous media, has an increased proportion of 2S canola protein and a decreased proportion of 7S canola protein. The novel canola protein isolate is formed by isoelectric precipitation of aqueous supernatant from canola protein micelle formation and precipitation, to effect precipitation of 7S protein which is sedimented and removed.
摘要翻译： 主要由2S卡诺拉蛋白质组成的新型卡诺拉蛋白分离物在水性介质中具有相同的较好的溶解性和改善的澄清性能，具有增加的2S卡诺拉蛋白的比例和7S卡诺拉蛋白的比例降低。 通过从卡诺拉蛋白胶束形成和沉淀的水性上清液的等电沉淀形成新的卡诺拉蛋白分离物，以沉淀和去除7S蛋白质的沉淀。
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公开(公告)号：US20080289963A1
公开(公告)日：2008-11-27
申请号：US12096815
申请日：2006-12-19
申请人： Bengt Bjellqvist , Kristina Uhlen
发明人： Bengt Bjellqvist , Kristina Uhlen
IPC分类号： C07K1/28
CPC分类号： C07K1/28 , G01N27/44747 , G01N27/44795
摘要： The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.
摘要翻译： 本发明涉及电泳领域，更具体地涉及通过在聚焦之前以新颖的方式生产IPG（固定化pH梯度）凝胶或条带，通过等电聚焦分离肽组分的凝胶或条带。 更紧密地，肽聚焦在包含具有至少三个分开的逐步排列的pH间隔的不均匀或非线性pH梯度的新型IPG凝胶中。 聚焦后肽分辨率高，肽沿凝胶均匀分布。
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公开(公告)号：US20070119712A1
公开(公告)日：2007-05-31
申请号：US10556196
申请日：2003-09-24
申请人： Detlev Hadbawnik
发明人： Detlev Hadbawnik
CPC分类号： G01N27/44795 , G01N27/44773
摘要： Disclosed is a separation of amphoteric molecules according to their isoelectric points, wherein a first separation according to the isoelectric points of the molecules in one ore more first strip-like separation media located on a first carrier is carried out. First separation media have a first pl range.
摘要翻译： 公开了根据其等电点的两性分子的分离，其中进行根据位于第一载体上的一个或多个第一条带状分离介质中的分子的等电点的第一分离。 第一分离介质具有第一个P1范围。
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