公开(公告)号：US20240209021A1

公开(公告)日：2024-06-27

申请号：US18540975

申请日：2023-12-15

申请人： Regeneron Pharmaceuticals, Ins.

发明人： Isabelle LIVIGNI ,  Stefanie MCDERMOTT ,  James REILLY ,  John MATTILA

IPC分类号： C07K1/107 ,  B01D15/18 ,  B01D15/30 ,  C07K1/20 ,  C07K16/06

CPC分类号： C07K1/1077 ,  B01D15/1864 ,  B01D15/305 ,  C07K1/20 ,  C07K16/065

摘要： Embodiments of the present disclosure are directed to methods for preparing a target polypeptide from a mixture including the target polypeptide. The method may include contacting the mixture to a hydrophobic interaction chromatography (HIC) apparatus including multiple chromatographic zones. The method may further include passing the target polypeptide through the outlets of at least a first zone and a second zone of the HIC apparatus. A residence time for the mixture including the target polypeptide in a first zone may be approximately the same as a residence time of one or more mobile phases in the second zone.