公开(公告)号：US20210040507A1

公开(公告)日：2021-02-11

申请号：US16979722

申请日：2019-03-13

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Branden Moriarity ,  Beau Webber ,  Cara-Lin Lonetree ,  Miechaleen Diers ,  Mitchell Kluesner ,  Walker Lahr ,  Emily Joy Pomeroy

IPC分类号： C12N15/90 ,  C12N5/0783

摘要： Provided herein are methods and systems for targeted gene disruption (knock-out, missense mutation) and targeted gene knock-in in mammalian cells using base editors and guide RNAs (gRNAs) designed to target splice acceptor-splice donor sites. Also provided herein are universally acceptable genetically engineered cells comprising targeted disruptions in immunotherapy-related genes and comprising a CAR/TCR for therapeutic applications.

公开(公告)号：US20220002717A1

公开(公告)日：2022-01-06

申请号：US17290968

申请日：2019-11-08

申请人： Regents of the University of Minnesota

发明人： Branden Moriarity ,  Mitchell Kluesner ,  Beau Webber

IPC分类号： C12N15/11 ,  C12N9/22 ,  C12N9/24 ,  C12N9/78 ,  C12N9/06

摘要： Provided herein are methods and compositions for highly precise base editing and single strand nicking. In particular, provided herein are methods for producing a genetically modified cell where the methods employ a universal, highly precise base editor or staggered Cas9 editor for precise base editing with minimal off-target or bystander effects.