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11 条记录 (耗时 0.032 秒)

    Host cells transformed with the E. coli glucoronide permease gene
    4.
    发明授权
    Host cells transformed with the E. coli glucoronide permease gene 失效
    宿主细胞用大肠杆菌葡萄糖醛酸通透酶基因转化

    公开(公告)号：US5432081A

    公开(公告)日：1995-07-11

    申请号：US138546

    申请日：1993-10-15

    申请人： Richard A. Jefferson

    发明人： Richard A. Jefferson

    IPC分类号： C07K14/245 ,  C12N9/24 ,  C12N15/56 ,  C12N15/82 ,  C12N15/85 ,  C12N1/21 ,  C12N5/10

    CPC分类号： C12Y302/01031 ,  C07K14/245 ,  C12N15/8209 ,  C12N15/85 ,  C12N9/2434 ,  C07K2319/00 ,  C07K2319/61 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/65

    摘要： The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.

    摘要翻译： 本发明涉及β-葡糖醛酸糖苷酶（GUS）基因融合系统，以及克隆和表征大肠杆菌的β-葡糖苷酸酶和葡糖苷酸通透酶基因。 基于惊人的发现，包含β-葡糖醛酸糖苷酶基因的基因融合物可以在多种生物体中有效表达以产生活性β-葡萄糖醛酸酶。 由于β-葡萄糖醛酸酶的有用底物的丰富性和可用性，GUS基因融合可以作为优异的报道基因系统以及改变细胞表型的有效手段。 结合可用于使宿主细胞可渗透β-葡萄糖醛酸酶底物的重组葡糖苷酸通透酶，GUS基因融合系统在植物和动物基因工程领域提供几乎无限的应用。

    .beta.-glucuronidase and glucuronide permease gene system
    6.
    发明授权
    .beta.-glucuronidase and glucuronide permease gene system 失效
    β-葡萄糖醛酸苷酶和葡糖苷酸通透酶基因系统

    公开(公告)号：US5599670A

    公开(公告)日：1997-02-04

    申请号：US329701

    申请日：1995-02-21

    申请人： Richard A. Jefferson

    发明人： Richard A. Jefferson

    IPC分类号： C07K14/245 ,  C12N9/24 ,  C12N15/56 ,  C12N15/82 ,  C12N15/85 ,  C12Q1/68 ,  A61K48/00 ,  C12N9/00

    CPC分类号： C12Y302/01031 ,  C07K14/245 ,  C12N15/8209 ,  C12N15/85 ,  C12N9/2434 ,  C07K2319/00 ,  C07K2319/61 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/65

    摘要： The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.

    摘要翻译： 本发明涉及β-葡糖醛酸糖苷酶（GUS）基因融合系统，以及克隆和表征大肠杆菌的β-葡糖苷酸酶和葡糖苷酸通透酶基因。 基于惊人的发现，包含β-葡糖醛酸糖苷酶基因的基因融合物可以在多种生物体中有效表达以产生活性β-葡萄糖醛酸酶。 由于β-葡萄糖醛酸酶的有用底物的丰富性和可用性，GUS基因融合可以作为优异的报道基因系统以及改变细胞表型的有效手段。 结合可用于使宿主细胞可渗透β-葡萄糖醛酸酶底物的重组葡糖苷酸通透酶，GUS基因融合系统在植物和动物基因工程领域提供几乎无限的应用。

    Plant promoter .alpha.-glucuronidase gene construct
    7.
    发明授权
    Plant promoter .alpha.-glucuronidase gene construct 失效
    植物启动子β-葡糖苷酸酶基因构建体

    公开(公告)号：US5268463A

    公开(公告)日：1993-12-07

    申请号：US447976

    申请日：1989-12-08

    申请人： Richard A. Jefferson

    发明人： Richard A. Jefferson

    IPC分类号： C07K14/245 ,  C12N9/24 ,  C12N15/56 ,  C12N15/82 ,  C12N15/85

    CPC分类号： C12Y302/01031 ,  C07K14/245 ,  C12N15/8209 ,  C12N15/85 ,  C12N9/2434 ,  C07K2319/00 ,  C07K2319/61 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12N2830/55 ,  C12N2830/65

    摘要： The present invention relates to the .beta.-glucuronidase (GUS) gene fusion system, and to the cloning and characterization of the .beta.-glucuronidase and glucuronide permease genes of Escherichia coli. It is based on the surprising discovery that gene fusions comprising the .beta.-glucuronidase gene may be effectively expressed in a wide variety of organisms to produce active .beta.-glucuronidase enzyme. Because of the abundance and availability of useful substrates for .beta.-glucuronidase enzyme, GUS gene fusions may serve as a superior reporter gene system as well as an effective means of altering cellular phenotype. In conjunction with recombinant glucuronide permease, which may be used to render host cells permeable to .beta.-glucuronidase substrates, the GUS gene fusion system offers almost unlimited applications in the fields of plant and animal genetic engineering.

    摘要翻译： 本发明涉及β-葡糖醛酸糖苷酶（GUS）基因融合系统，以及克隆和表征大肠杆菌的β-葡糖苷酸酶和葡糖苷酸通透酶基因。 基于惊人的发现，包含β-葡糖醛酸糖苷酶基因的基因融合物可以在多种生物体中有效表达以产生活性β-葡萄糖醛酸酶。 由于β-葡萄糖醛酸酶的有用底物的丰富性和可用性，GUS基因融合可以作为优异的报道基因系统以及改变细胞表型的有效手段。 结合可用于使宿主细胞可渗透β-葡萄糖醛酸酶底物的重组葡糖苷酸通透酶，GUS基因融合系统在植物和动物基因工程领域提供几乎无限的应用。

    Preparation of cellobiuronic acid from polysaccharide
    10.
    发明授权
    Preparation of cellobiuronic acid from polysaccharide 失效
    从多糖制备纤维二糖酸

    公开(公告)号：US06268493B1

    公开(公告)日：2001-07-31

    申请号：US09130695

    申请日：1998-08-07

    申请人： Richard A. Jefferson

    发明人： Richard A. Jefferson

    IPC分类号： C07H106

    CPC分类号： C07H1/08 ,  C07H13/02

    摘要： Cellobiuronic acid may be prepared by hydrolysis of gellan. The method provides embodiments expressed in terms of amounts of cellobiuronic acid produced, amounts of gellan gum hydrolyzed and amounts of cellobiuronic acid present in fractions of a hydrolysate The method may further include isolation of a separated fraction of the hydrolysate where the separated fraction comprises cellobiuronic acid. A preferred embodiment of the method includes hydrolyzing gellan gum with a protic acid under reaction conditions that convert at least 95 wt. % of the gellan gum to a hydrolysate comprised of cellobiuronic acid and monosaccharides and isolating a separated fraction of the hydrolysate where cellobiuronic acid comprises at least a 95 wt. % of saccharides in the separated fraction.

    摘要翻译： 纤维二糖酸可以通过水解吉兰糖来制备。 该方法提供以所产生的纤维二糖酸的量，水解的结冷胶胶的量和存在于水解产物的级分中的纤维二糖酸的量表示的实施方案。该方法还可以包括分离水解产物的分离级分，其中分离的级分包含纤维二糖酸 。 该方法的优选实施方案包括在反应条件下水解具有质子酸的结冷胶，其转化率至少为95wt。 ％的吉兰糖胶与由纤维二糖醛酸和单糖组成的水解产物，并分离水解产物的分离部分，其中纤维二糖酸包含至少95重量％ 分离级分中的糖的百分比。