公开(公告)号：US5219761A

公开(公告)日：1993-06-15

申请号：US716430

申请日：1991-06-17

申请人： Richard K. Lankow ,  Sally A. Miller ,  G. David Grothaus ,  Frank P. Petersen ,  Dennis R. Stocker ,  Stephanie L. Papa ,  James Donovan ,  Douglas Malik

发明人： Richard K. Lankow ,  Sally A. Miller ,  G. David Grothaus ,  Frank P. Petersen ,  Dennis R. Stocker ,  Stephanie L. Papa ,  James Donovan ,  Douglas Malik

IPC分类号： G01N33/52 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N33/521 ,  G01N33/543 ,  G01N33/54386 ,  G01N33/56961 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

摘要： A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.

摘要翻译： 一种用于检测可疑抗原植物的方法和试剂盒。 在第一和第二垫之间取出并摩擦植物的样品以从样品中提取汁液并在第一垫上收集汁液。 将汁液转移到液体溶液中，并测试该溶液中的疑似抗原。 这可以通过使溶液与标签抗体和具有捕获抗体的载体接触来实现，使得如果可疑抗原在液体溶液中，则在载体上形成标签抗体 - 抗原 - 捕获抗体复合物。 然后测试载体的标签抗体。 试剂盒优选包括第一和第二垫，载体，用于保持液体溶液的容器和标签抗体，以及测试载体用于标签抗体的装置或试剂。

公开(公告)号：US5047207A

公开(公告)日：1991-09-10

申请号：US865379

申请日：1986-05-20

申请人： Richard K. Lankow ,  Sally A. Miller ,  G. David Grothaus ,  Frank P. Petersen ,  Dennis R. Stocker ,  Stephanie L. Papa ,  James Donovan ,  Douglas Malik

发明人： Richard K. Lankow ,  Sally A. Miller ,  G. David Grothaus ,  Frank P. Petersen ,  Dennis R. Stocker ,  Stephanie L. Papa ,  James Donovan ,  Douglas Malik

IPC分类号： G01N33/53 ,  G01N33/52 ,  G01N33/543 ,  G01N33/569

CPC分类号： G01N33/521 ,  G01N33/543 ,  G01N33/54386 ,  G01N33/56961

摘要： A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.

摘要翻译： 一种用于检测可疑抗原植物的方法和试剂盒。 在第一和第二垫之间取出并摩擦植物的样品以从样品中提取汁液并在第一垫上收集汁液。 将汁液转移到液体溶液中，并测试该溶液中的疑似抗原。 这可以通过使溶液与标签抗体和具有捕获抗体的载体接触来实现，使得如果可疑抗原在液体溶液中，则在载体上形成标签抗体 - 抗原 - 捕获抗体复合物。 然后测试载体的标签抗体。 试剂盒优选包括第一和第二垫，载体，用于保持液体溶液的容器和标签抗体，以及测试载体用于标签抗体的装置或试剂。