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公开(公告)号:US06402950B1
公开(公告)日:2002-06-11
申请号:US09665860
申请日:2000-09-20
申请人: Paul T. Nix , Dennis R. Stocker , Michael J. Byers
发明人: Paul T. Nix , Dennis R. Stocker , Michael J. Byers
IPC分类号: B01D3500
CPC分类号: B01L3/50255 , B01L2400/0409 , Y10T436/25375 , Y10T436/255
摘要: A device which can be used for filtering samples is described. The device is comprised of a plurality of filter wells, each having a wide region and a narrow region. The device is fitted with a filter element and then filled with a gel filtration matrix which is selected so that molecules of a particular molecular size will diffuse into the matrix. The device is centrifuged to remove water from the interstitial regions of the gel filtration matrix, and a sample is then added to the device containing the dried gel filtration matrix. The device is again centrifuged, causing smaller molecules to diffuse into the matrix and allowing larger molecules to exit the device, wherein they are collected and analyzed. The device is capable of filtering sample sizes as low as 1 &mgr;L and can be used in a variety of existing laboratory equipment, including bench-top centrifuges and microcentrifuges. A process for using the device is also disclosed.
摘要翻译: 描述可用于过滤样品的装置。 该装置由多个过滤器孔组成,每个过滤器孔具有宽的区域和窄的区域。 该装置装有过滤元件,然后填充凝胶过滤基质,其被选择使得特定分子尺寸的分子将扩散到基质中。 将装置离心以从凝胶过滤基质的间隙区域除去水,然后将样品加入到含有干燥的凝胶过滤基质的装置中。 该装置再次离心,导致较小的分子扩散到基质中并允许较大的分子离开该装置,其中它们被收集和分析。 该设备能够过滤低至1μL的样品量,可用于各种现有的实验室设备,包括台式离心机和微量离心机。 还公开了一种使用该装置的方法。
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公开(公告)号:US5219761A
公开(公告)日:1993-06-15
申请号:US716430
申请日:1991-06-17
申请人: Richard K. Lankow , Sally A. Miller , G. David Grothaus , Frank P. Petersen , Dennis R. Stocker , Stephanie L. Papa , James Donovan , Douglas Malik
发明人: Richard K. Lankow , Sally A. Miller , G. David Grothaus , Frank P. Petersen , Dennis R. Stocker , Stephanie L. Papa , James Donovan , Douglas Malik
IPC分类号: G01N33/52 , G01N33/543 , G01N33/569
CPC分类号: G01N33/521 , G01N33/543 , G01N33/54386 , G01N33/56961 , Y10T436/25 , Y10T436/25375 , Y10T436/255
摘要: A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.
摘要翻译: 一种用于检测可疑抗原植物的方法和试剂盒。 在第一和第二垫之间取出并摩擦植物的样品以从样品中提取汁液并在第一垫上收集汁液。 将汁液转移到液体溶液中,并测试该溶液中的疑似抗原。 这可以通过使溶液与标签抗体和具有捕获抗体的载体接触来实现,使得如果可疑抗原在液体溶液中,则在载体上形成标签抗体 - 抗原 - 捕获抗体复合物。 然后测试载体的标签抗体。 试剂盒优选包括第一和第二垫,载体,用于保持液体溶液的容器和标签抗体,以及测试载体用于标签抗体的装置或试剂。
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公开(公告)号:US5047207A
公开(公告)日:1991-09-10
申请号:US865379
申请日:1986-05-20
申请人: Richard K. Lankow , Sally A. Miller , G. David Grothaus , Frank P. Petersen , Dennis R. Stocker , Stephanie L. Papa , James Donovan , Douglas Malik
发明人: Richard K. Lankow , Sally A. Miller , G. David Grothaus , Frank P. Petersen , Dennis R. Stocker , Stephanie L. Papa , James Donovan , Douglas Malik
IPC分类号: G01N33/53 , G01N33/52 , G01N33/543 , G01N33/569
CPC分类号: G01N33/521 , G01N33/543 , G01N33/54386 , G01N33/56961
摘要: A method and a kit for testing a plant for a suspected antigen. A sample of the plant is taken and rubbed between first and second pads to extract sap from the sample and to collect the sap on the first pad. The sap is transferred to a liquid solution, and that solution is tested for the suspected antigen. This may be done by contacting the solution with a tag antibody and with a carrier having a capture antibody so that a tag antibody-antigen-capture antibody complex forms on the carrier if the suspected antigen is in the liquid solution. The carrier is then tested for the tag antibody. The kit preferably includes the first and second pads, the carrier, containers to hold the liquid solution and the tag antibody, and a device or reagent to test the carrier for the tag antibody.
摘要翻译: 一种用于检测可疑抗原植物的方法和试剂盒。 在第一和第二垫之间取出并摩擦植物的样品以从样品中提取汁液并在第一垫上收集汁液。 将汁液转移到液体溶液中,并测试该溶液中的疑似抗原。 这可以通过使溶液与标签抗体和具有捕获抗体的载体接触来实现,使得如果可疑抗原在液体溶液中,则在载体上形成标签抗体 - 抗原 - 捕获抗体复合物。 然后测试载体的标签抗体。 试剂盒优选包括第一和第二垫,载体,用于保持液体溶液的容器和标签抗体,以及测试载体用于标签抗体的装置或试剂。
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