Biocomposite comprising co-precipitate of enzyme, silicate and polyamine
    1.
    发明授权
    Biocomposite comprising co-precipitate of enzyme, silicate and polyamine 有权
    生物复合物包括酶,硅酸盐和多胺的共沉淀物

    公开(公告)号:US07642077B2

    公开(公告)日:2010-01-05

    申请号:US11007437

    申请日:2004-12-07

    IPC分类号: C12N11/14 C12N11/02 C12N11/04

    CPC分类号: C12N9/54 C12N11/08 C12N11/14

    摘要: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, δ-hydroxylysine, and δ-hydroxy-ω-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.

    摘要翻译: 本发明涉及通过有机模板分子的作用,通过与硅酸盐或有机硅酸盐基质共沉淀来固定包括蛋白质和细胞的生物催化剂的方法。 有机模板分子通常是多胺,例如聚乙烯亚胺(PEI)或具有选自赖氨酸,精氨酸,组氨酸,脯氨酸,羟脯氨酸,N-甲基组氨酸,鸟氨酸,牛磺酸中的至少两个或三个碱性残基的多肽化合物 ,δ-羟基赖氨酸和δ-羟基-ω-N,N,N三甲基赖氨酸。 本发明还涉及包含活性生物催化剂,二氧化硅或有机硅酸盐和含N有机模板分子的共沉淀物的二氧化硅生物复合物。 这种二氧化硅生物复合材料可用于生物催化和其它需要固定化生物催化剂的应用。 本发明优选的生物催化剂是酶和全细胞。

    IMMOBILIZATION OF BIOCATALYSTS BY TEMPLATE-DIRECTED SILICATE PRECIPITATION
    2.
    发明申请
    IMMOBILIZATION OF BIOCATALYSTS BY TEMPLATE-DIRECTED SILICATE PRECIPITATION 有权
    通过模板指导的硅酸盐沉淀法生物活性物质的稳定化

    公开(公告)号:US20090170180A1

    公开(公告)日:2009-07-02

    申请号:US12395242

    申请日:2009-02-27

    IPC分类号: C12N11/08

    CPC分类号: C12N9/54 C12N11/08 C12N11/14

    摘要: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, δ-hydroxylysine, and δ-hydroxy-ω-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.

    摘要翻译: 本发明涉及通过有机模板分子的作用,通过与硅酸盐或有机硅酸盐基质共沉淀来固定包括蛋白质和细胞的生物催化剂的方法。 有机模板分子通常是多胺,例如聚乙烯亚胺(PEI)或具有选自赖氨酸,精氨酸,组氨酸,脯氨酸,羟脯氨酸,N-甲基组氨酸,鸟氨酸,牛磺酸中的至少两个或三个碱性残基的多肽化合物 ,δ-羟基赖氨酸和δ-羟基-ω-N,N,N三甲基赖氨酸。 本发明还涉及包含活性生物催化剂,二氧化硅或有机硅酸盐和含N有机模板分子的共沉淀物的二氧化硅生物复合物。 这种二氧化硅生物复合材料可用于生物催化和其它需要固定化生物催化剂的应用。 本发明优选的生物催化剂是酶和全细胞。

    Method for preparing a biocomposite comprising one or more enzymes
    3.
    发明授权
    Method for preparing a biocomposite comprising one or more enzymes 有权
    制备包含一种或多种酶的生物复合材料的方法

    公开(公告)号:US08574880B2

    公开(公告)日:2013-11-05

    申请号:US12395242

    申请日:2009-02-27

    CPC分类号: C12N9/54 C12N11/08 C12N11/14

    摘要: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, δ-hydroxylysine, and δ-hydroxy-ω-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.

    摘要翻译: 本发明涉及通过有机模板分子的作用,通过与硅酸盐或有机硅酸盐基质共沉淀来固定包括蛋白质和细胞的生物催化剂的方法。 有机模板分子通常是多胺,例如聚乙烯亚胺(PEI)或具有选自赖氨酸,精氨酸,组氨酸,脯氨酸,羟脯氨酸,N-甲基组氨酸,鸟氨酸,牛磺酸中的至少两个或三个碱性残基的多肽化合物 ,δ-羟基赖氨酸和δ-羟基-ω-N,N,N三甲基赖氨酸。 本发明还涉及包含活性生物催化剂,二氧化硅或有机硅酸盐和含N有机模板分子的共沉淀物的二氧化硅生物复合物。 这种二氧化硅生物复合材料可用于生物催化和其它需要固定化生物催化剂的应用。 本发明优选的生物催化剂是酶和全细胞。

    Immobilization of biocatalysts by template-directed silicate precipitation
    6.
    发明申请
    Immobilization of biocatalysts by template-directed silicate precipitation 有权
    通过模板导向的硅酸盐沉淀固定生物催化剂

    公开(公告)号:US20050158837A1

    公开(公告)日:2005-07-21

    申请号:US11007437

    申请日:2004-12-07

    CPC分类号: C12N9/54 C12N11/08 C12N11/14

    摘要: This invention relates to a method of immobilizing biocatalysts including protein and cells by co-precipitation with silicate or organosilicate matrices through the action of an organic template molecule. The organic template molecule is in general a polyamine such as polyethylenimine (PEI), or polypeptide compound bearing at least two or three basic residues selected from the group consisting of lysine, arginine, histidine, proline, hydroxyproline, N-methylhistidine, ornithine, taurine, δ-hydroxylysine, and δ-hydroxy-ω-N,N,N trimethyllysine. The invention is also directed to a silica biocomposite comprising co-precipitates of active biocatalysts, silica or organosilicates, and an N-containing organic template molecule. Such silica biocomposites are useful in biocatalysis, and other applications requiring an immobilized biocatalyst. Preferred biocatalysts for this invention are enzymes and whole cells.

    摘要翻译: 本发明涉及通过有机模板分子的作用,通过与硅酸盐或有机硅酸盐基质共沉淀来固定包括蛋白质和细胞的生物催化剂的方法。 有机模板分子通常是多胺,例如聚乙烯亚胺(PEI)或具有选自赖氨酸,精氨酸,组氨酸,脯氨酸,羟脯氨酸,N-甲基组氨酸,鸟氨酸,牛磺酸中的至少两个或三个碱性残基的多肽化合物 ,δ-羟基赖氨酸和δ-羟基-ω-N,N,N三甲基赖氨酸。 本发明还涉及包含活性生物催化剂,二氧化硅或有机硅酸盐和含N有机模板分子的共沉淀物的二氧化硅生物复合物。 这种二氧化硅生物复合材料可用于生物催化和其它需要固定化生物催化剂的应用。 本发明优选的生物催化剂是酶和全细胞。