公开(公告)号：US08460744B2

公开(公告)日：2013-06-11

申请号：US12713105

申请日：2010-02-25

申请人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

发明人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

IPC分类号： B05D1/00

CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

摘要翻译： 一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。 还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。 该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及用于在照射期间向转印膜施加恒定压力的装置。

公开(公告)号：US20100216166A1

公开(公告)日：2010-08-26

申请号：US12713105

申请日：2010-02-25

申请人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

发明人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

IPC分类号： G01N33/567

CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

摘要翻译： 一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。 还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。 该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及在照射期间向转印膜施加恒定压力的装置。

公开(公告)号：US07695752B2

公开(公告)日：2010-04-13

申请号：US11202848

申请日：2005-08-12

申请人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

发明人： Robert F. Bonner ,  Thomas J. Pohida ,  Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Rodrigo F. Chuaqui

IPC分类号： B05D1/00

CPC分类号： G01N33/543 ,  G01N33/56966 ,  G01N2001/284 ,  Y10T428/2813

摘要： A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation.

摘要翻译： 一种用于执行目标激活转移的装置，其包括用于安装组织样本的安装表面; 以及光源，其定位成基本均匀地照射组织样本的染色和未染色区域，所述光能激发试剂以选择性地将染色区域粘附到转移表面。 还描述了一种用于将组织从组织样品转移到转移衬底的自动化系统。 该系统包括用于保持组织部分的装置，该组织部分包括用吸收性污渍特异性染色的靶，从而导致染色的组织表面;以及柔性转移膜，其包括与染色的组织表面充分热接触的下部热塑性层; 照射组件，被配置为向整个组织部分提供预定的均匀光剂量; 以及用于在照射期间向转印膜施加恒定压力的装置。

公开(公告)号：US08597715B2

公开(公告)日：2013-12-03

申请号：US12753566

申请日：2010-04-02

申请人： Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Robert F. Bonner ,  Rodrigo Chuaqui ,  Thomas J. Pohida

发明人： Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Robert F. Bonner ,  Rodrigo Chuaqui ,  Thomas J. Pohida

IPC分类号： B05D1/00

CPC分类号： G01N33/56966 ,  G01N33/543 ,  Y10T428/2813 ,  Y10T428/2839

摘要： A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorophore that absorbs light and emits heat.

摘要翻译： 一种从生物样品中除去靶的方法，其包括将转移表面与生物样品接触，然后焦点改变转移表面以允许靶与生物样品的选择性分离。 在所公开的实施方案中，靶是组织切片的细胞或细胞组分，并且转移表面是可以被焦点改变以将靶附着到转移表面的膜。 随后从组织切片分离膜，从组织切片选择性地去除粘附的靶。 转移表面从靶内活化，以将靶附着到转印表面，例如通过加热靶以将其粘附到热塑性转印表面。 可以通过将生物样品暴露于特异性结合靶（或靶的成分）的免疫反应物来实现这种原位激活。 免疫反应物可以直接（例如用免疫反应物携带的发热酶）或间接地（例如通过改变靶的特征）来改变转移表面。 在一些实施方案中，免疫反应物在靶中沉积沉淀物，其相对于周围组织增加其光吸收，使得生物样品可暴露于光以选择性加热靶标。 或者，免疫反应剂是携带吸收光并发出热量的荧光团的免疫荧光剂。

公开(公告)号：US20100190177A1

公开(公告)日：2010-07-29

申请号：US12753566

申请日：2010-04-02

申请人： Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Robert F. Bonner ,  Rodrigo Chuaqui ,  Thomas J. Pohida

发明人： Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Robert F. Bonner ,  Rodrigo Chuaqui ,  Thomas J. Pohida

IPC分类号： C12Q1/68 ,  B01L3/00 ,  G01N33/53 ,  G01N1/28 ,  G01N33/566

CPC分类号： G01N33/56966 ,  G01N33/543 ,  Y10T428/2813 ,  Y10T428/2839

摘要： A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorophore that absorbs light and emits heat.

摘要翻译： 一种从生物样品中除去靶的方法，其包括将转移表面与生物样品接触，然后焦点改变转移表面以允许靶与生物样品的选择性分离。 在所公开的实施方案中，靶是组织切片的细胞或细胞组分，并且转移表面是可以被焦点改变以将靶附着到转移表面的膜。 随后从组织切片分离膜，从组织切片选择性地去除粘附的靶。 转移表面从靶内活化，以将靶附着到转印表面，例如通过加热靶以将其粘附到热塑性转印表面。 可以通过将生物样品暴露于特异性结合靶（或靶的成分）的免疫反应物来实现这种原位激活。 免疫反应物可以直接（例如用免疫反应物携带的发热酶）或间接地（例如通过改变靶的特征）来改变转移表面。 在一些实施方案中，免疫反应物在靶中沉积沉淀物，其相对于周围组织增加其光吸收，使得生物样品可暴露于光以选择性加热靶标。 或者，免疫反应剂是携带吸收光并发出热量的荧光团的免疫荧光剂。

公开(公告)号：US07709047B2

公开(公告)日：2010-05-04

申请号：US10543218

申请日：2003-07-23

申请人： Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Robert F. Bonner ,  Rodrigo Chuaqui ,  Thomas J. Pohida

发明人： Michael R. Emmert-Buck ,  Michael Anthony Tangrea ,  Robert F. Bonner ,  Rodrigo Chuaqui ,  Thomas J. Pohida

IPC分类号： B05D1/00

CPC分类号： G01N33/56966 ,  G01N33/543 ,  Y10T428/2813 ,  Y10T428/2839

摘要： A method of removing a target from a biological sample which involves placing a transfer surface in contact with the biological sample, and then focally altering the transfer surface to allow selective separation of the target from the biological sample. In disclosed embodiments, the target is a cell or cellular component of a tissue section and the transfer surface is a film that can be focally altered to adhere the target to the transfer surface. Subsequent separation of the film from the tissue section selectively removes the adhered target from the tissue section. The transfer surface is activated from within the target to adhere the target to the transfer surface, for example by heating the target to adhere it to a thermoplastic transfer surface. Such in situ activation can be achieved by exposing the biological sample to an immunoreagent that specifically binds to the target (or a component of the target). The immunoreagent can alter the transfer surface directly (for example with a heat generating enzyme carried by the immunoreagent), or indirectly (for example by changing a characteristic of the target). In some embodiments, the immunoreagent deposits a precipitate in the target that increases its light absorption relative to surrounding tissue, such that the biological specimen can be exposed to light to selectively heat the target. Alternatively, the immunoreagent is an immunofluorescent agent that carries a fluorophore that absorbs light and emits heat.

摘要翻译： 一种从生物样品中除去靶的方法，其包括将转移表面与生物样品接触，然后焦点改变转移表面以允许靶与生物样品的选择性分离。 在所公开的实施方案中，靶是组织切片的细胞或细胞组分，并且转移表面是可以被焦点改变以将靶附着到转移表面的膜。 随后从组织切片分离膜，从组织切片选择性地去除粘附的靶。 转移表面从靶内活化，以将靶附着到转印表面，例如通过加热靶以将其粘附到热塑性转印表面。 可以通过将生物样品暴露于特异性结合靶（或靶的成分）的免疫反应物来实现这种原位激活。 免疫反应物可以直接（例如用免疫反应物携带的发热酶）或间接地（例如通过改变靶的特征）来改变转移表面。 在一些实施方案中，免疫反应物在靶中沉积沉淀物，其相对于周围组织增加其光吸收，使得生物样品可暴露于光以选择性加热靶标。 或者，免疫反应剂是携带吸收光并发出热量的荧光团的免疫荧光剂。