公开(公告)号：US5637323A

公开(公告)日：1997-06-10

申请号：US341399

申请日：1994-11-16

申请人： Robert H. Wiltrout ,  Francis Ruscetti ,  Krzysztof Grzegorzewski ,  Jonathan Keller ,  Kristin L. Komschlies-McConville

发明人： Robert H. Wiltrout ,  Francis Ruscetti ,  Krzysztof Grzegorzewski ,  Jonathan Keller ,  Kristin L. Komschlies-McConville

IPC分类号： A61K35/12 ,  A61K38/00 ,  A61K38/20 ,  C12N5/0789 ,  C12N15/85 ,  A61K35/14 ,  A01N63/00

CPC分类号： C12N5/0647 ,  A61K38/2046 ,  A61K2035/124

摘要： This invention provides a method of isolating an increased number of hematopoietic stem cells from a subject comprising a) administering interleukin-7 to the subject in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; and, b) isolating a population of leukocytes enriched for hematopoietic stem cells from the peripheral blood. Also provided is a method of transplanting an increased number of hematopoietic stem cells from a donor to a recipient to enhance repopulation of the recipient's hematopoietic and immune cells comprising a) administering interleukin-7 to the donor in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; b) isolating a population of leukocytes enriched for hematopoietic stem cells from the donor's peripheral blood; and, c) transplanting the isolated population of leukocytes enriched for hematopoietic stem cells to the recipient, thereby enhancing the repopulation of the recipient's hematopoietic and immune cells. Finally, the invention provides a method of improving engraftment of a bone marrow transplant or a peripheral blood leukocyte transplant from a donor to a recipient comprising administering interleukin-7 to the recipient following transplantation in an amount that enhances engraftment.

摘要翻译： 本发明提供了从受试者中分离增加数目的造血干细胞的方法，其包括a）以将造血干细胞移植到外周血的量向受试者施用白细胞介素-7; 和b）从外周血中分离富含造血干细胞的白细胞群。 还提供了将增加数量的造血干细胞从供体移植到受体以增强受体的造血细胞和免疫细胞的再增殖的方法，其包括a）以使动员造血干细胞的量向供体施用白细胞介素-7 外周血; b）从供体的外周血中分离富含造血干细胞的白细胞群; 并且c）将分离的富含造血干细胞的白细胞群体移植到受体，从而增强受体的造血细胞和免疫细胞的再增殖。 最后，本发明提供了一种改善骨髓移植物或外周血白细胞移植物从供体到接受者的移植的方法，其包括以增强移植量的量将移植后的白细胞介素-7给予受体。