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公开(公告)号：US08532398B2
公开(公告)日：2013-09-10
申请号：US12732272
申请日：2010-03-26
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/06 , G02B21/365
摘要： A method for the imaging of protein expression and location in biological samples using optical segmentation is provided. The steps comprise acquiring a fluorescent image of a biological sample, analyzing the image and generating a masking pattern corresponding to a specific structure within the biological sample, transforming the masking pattern into the spatial coordinates of a digital micro-mirror device (DMD) which may then be projected onto the biological sample and obtaining a masked fluorescent image. Also provided is an image analysis system for imaging of protein expression and location in biological samples using optical segmentation.
摘要翻译： 提供了一种使用光学分割技术在生物样品中成像蛋白质表达和定位的方法。 步骤包括获取生物样品的荧光图像，分析图像并产生对应于生物样品内的特定结构的掩模图案，将掩模图案变换成数字微镜装置（DMD）的空间坐标，其可以 然后投射到生物样品上并获得掩蔽的荧光图像。 还提供了一种用于使用光学分割对生物样品中蛋白质表达和位置成像进行成像的图像分析系统。
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公开(公告)号：US20110235875A1
公开(公告)日：2011-09-29
申请号：US12732272
申请日：2010-03-26
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/06 , G02B21/365
摘要： A method for the imaging of protein expression and location in biological samples using optical segmentation is provided. The steps comprise acquiring a fluorescent image of a biological sample, analyzing the image and generating a masking pattern corresponding to a specific structure within the biological sample, transforming the masking pattern into the spatial coordinates of a digital micro-mirror device (DMD) which may then be projected onto the biological sample and obtaining a masked fluorescent image. Also provided is an image analysis system for imaging of protein expression and location in biological samples using optical segmentation.
摘要翻译： 提供了一种使用光学分割技术在生物样品中成像蛋白质表达和定位的方法。 步骤包括获取生物样品的荧光图像，分析图像并产生对应于生物样品内的特定结构的掩模图案，将掩模图案变换成数字微镜装置（DMD）的空间坐标，其可以 然后投射到生物样品上并获得掩蔽的荧光图像。 还提供了一种用于使用光学分割对生物样品中蛋白质表达和位置成像进行成像的图像分析系统。
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公开(公告)号：US20080266440A1
公开(公告)日：2008-10-30
申请号：US11843754
申请日：2007-08-23
申请人： Siavash Yazdanfar , Robert John Filkins , Elizabeth Lokenberg Dixon , Walter Vincent Dixon , Krenar Tasimi , Kevin Bernard Kenny
发明人： Siavash Yazdanfar , Robert John Filkins , Elizabeth Lokenberg Dixon , Walter Vincent Dixon , Krenar Tasimi , Kevin Bernard Kenny
CPC分类号： G02B21/244 , G02B21/365
摘要： A method of autofocusing includes capturing first, second and third images of a sample, at respective first, second and third sample distances and respective first, second and third lateral positions determined with respect to an objective; determining a quantitative characteristic for the first, second and third images; determining a primary sample distance based upon at least the quantitative characteristics for the first, second, and third images; and capturing a primary image of the sample at the primary sample distance and at a primary lateral position that is offset from the first, second and third lateral positions.
摘要翻译： 自动对焦的方法包括：以相对于目标确定的各自的第一，第二和第三采样距离以及相应的第一，第二和第三横向位置捕获样本的第一，第二和第三图像; 确定第一，第二和第三图像的定量特性; 至少基于第一，第二和第三图像的定量特性来确定主样本距离; 以及在所述主要采样距离处和在偏离所述第一，第二和第三横向位置的主横向位置处捕获所述样本的主要图像。
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公开(公告)号：US08179432B2
公开(公告)日：2012-05-15
申请号：US11843754
申请日：2007-08-23
申请人： Siavash Yazdanfar , Robert John Filkins , Elizabeth Lockenberg Dixon , Walter Vincent Dixon, legal representative , Krenar Tasimi , Kevin Bernard Kenny
发明人： Siavash Yazdanfar , Robert John Filkins , Elizabeth Lockenberg Dixon , Krenar Tasimi , Kevin Bernard Kenny
CPC分类号： G02B21/244 , G02B21/365
摘要： A method of autofocusing includes capturing first, second and third images of a sample, at respective first, second and third sample distances and respective first, second and third lateral positions determined with respect to an objective; determining a quantitative characteristic for the first, second and third images; determining a primary sample distance based upon at least the quantitative characteristics for the first, second, and third images; and capturing a primary image of the sample at the primary sample distance and at a primary lateral position that is offset from the first, second and third lateral positions.
摘要翻译： 自动对焦的方法包括：以相对于目标确定的各自的第一，第二和第三采样距离以及相应的第一，第二和第三横向位置捕获样本的第一，第二和第三图像; 确定第一，第二和第三图像的定量特性; 至少基于第一，第二和第三图像的定量特性来确定主样本距离; 以及在所述主要采样距离处和在偏离所述第一，第二和第三横向位置的主横向位置处捕获所述样本的主要图像。
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