公开(公告)号：US20240142441A1

公开(公告)日：2024-05-02

申请号：US18541708

申请日：2023-12-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Regina Baranowski ,  Dieter Heindl ,  Nikolaus-Peter Stengele ,  Christian Wellner

IPC: G01N33/543 ,  G01R33/09

CPC classification number: G01N33/54306 ,  G01R33/098

Abstract: The present invention concerns the field of diagnostics. In particular, it relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with a sensor element comprising an anchor layer which is present on a solid support, a first binding agent which is capable of specifically binding to the analyte, which is anchored in the anchor layer, a second binding agent which is capable of specifically binding to the analyte when bound to the first binding agent and which is immobilized on the solid support, said second binding agent comprising at least one magnetic label, and a magnetic tunnel junction in functional proximity to the second binding agent which generates a signal elicited in proximity to the at least one magnetic label of the second binding agent, for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent and detecting the formation the complex of first binding agent, analyte and second binding agent based on an altered signal which is generated by the magnetic tunnel junction whereby the analyte is determined. The present invention, further, relates to a device and a kit for determining an analyte suspected to be present in a sample and to the use of said device for determining an analyte suspected to be present in a sample.

公开(公告)号：US20240310355A1

公开(公告)日：2024-09-19

申请号：US18672786

申请日：2024-05-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Martin Rempt ,  Christian Wellner

IPC: G01N33/487

CPC classification number: G01N33/48721

Abstract: The present invention relates to a method for determining an analyte of interest by frequency detection and the use thereof, a modified nanopore, an analyzing system, a kit and the uses thereof.

公开(公告)号：US20240118271A1

公开(公告)日：2024-04-11

申请号：US18541875

申请日：2023-12-15

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Regina Baranowski ,  Dieter Heindl ,  Nikolaus-Peter Stengele ,  Christian Wellner

IPC: G01N33/543

CPC classification number: G01N33/54326 ,  G01N2446/60

Abstract: The present invention relates to diagnostic test and technology. In particular, the present invention relates to a method for determining an analyte suspected to be present in a sample comprising contacting said sample with a sensor element comprising an anchor layer which is present on a solid support, a first binding agent which is capable of specifically binding to the analyte, which is anchored in the anchor layer and which comprises at least one magnetic label, wherein said at least one magnetic label is located within the anchor layer, a second binding agent which is capable of specifically binding to the analyte when bound to the first binding agent and which is immobilized on the solid support, and a magnetic tunnel junction in functional proximity to the second binding agent which generates a signal elicited in proximity to the at least one magnetic label of the first binding agent, for a time and under conditions which allow for specific binding of the analyte suspected to be present in the sample to the first binding agent and specific binding of the second binding agent to the analyte bound to the first binding agent and detecting the formation the complex of first binding agent, analyte and second binding agent based on the signal which is generated by the magnetic tunnel junction whereby the analyte is determined. Moreover, provided is a device for determining an analyte suspected to be present in a sample and the use thereof for determining an analyte suspected to be present in a sample in said sample. Moreover, the present invention contemplates a kit for determining an analyte suspected to be present in a sample.