公开(公告)号：US11320423B2

公开(公告)日：2022-05-03

申请号：US15995572

申请日：2018-06-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara Upmeier ,  Toralf Zarnt ,  Dieter Roessler ,  Johannes Polz

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/541 ,  G01N33/543 ,  G01N21/64 ,  G01N21/78 ,  G01N33/531 ,  G01N33/538 ,  G01N33/569 ,  G01N33/576

Abstract: Disclosed is an immunoassay method for detecting an analyte such as an antigen or an antibody in an isolated sample suspected to contain the analyte by incubating the sample with a plurality of binding partners, one of which carries a detectable label, wherein a label-specific binding partner is added that does not carry a label but binds to the detectable label. The method is applicable for a large variety of analytes and has proven particularly useful for analyte antibodies of the IgG and IgM class present in samples due to infections by pathogens. Also disclosed is a reagent kit useful for the method comprising at least two analyte-specific binding partners one of which carries a detectable label and a label-specific binding partner that binds to said detectable label but itself does not carry a detectable label.

公开(公告)号：US11156610B2

公开(公告)日：2021-10-26

申请号：US15621280

申请日：2017-06-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara Upmeier ,  Toralf Zarnt ,  Johannes Polz

IPC: G01N31/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/68

Abstract: Described is a method for determining an analyte in a sample suspected to contain the analyte, by a) contacting the sample with a first and a second capture compound for the analyte, wherein the first and second capture compounds are non-identical capture compounds, and the capture compounds compete in binding to the analyte; b) contacting the capture compounds contacted with the sample with a specifier, wherein the specifier competes in binding to the capture compounds with the analyte; c) determining the amount of complexes having the specifier and a capture compound; and d) determining the analyte in a sample based on the result of step c). Also disclosed is a method for improving the specificity of an indirect immunoassay for determining an analyte, as well as kits, devices, and uses related to the methods.

公开(公告)号：US20180348211A1

公开(公告)日：2018-12-06

申请号：US15995572

申请日：2018-06-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara Upmeier ,  Toralf Zarnt ,  Dieter Roessler ,  Johannes Polz

IPC: G01N33/541 ,  G01N21/64 ,  G01N33/538 ,  G01N21/78 ,  G01N33/531 ,  G01N33/53 ,  G01N33/543 ,  G01N33/576 ,  G01N33/569

CPC classification number: G01N33/54393 ,  G01N33/54306

Abstract: Disclosed is an immunoassay method for detecting an analyte such as an antigen or an antibody in an isolated sample suspected to contain the analyte by incubating the sample with a plurality of binding partners, one of which carries a detectable label, wherein a label-specific binding partner is added that does not carry a label but binds to the detectable label. The method is applicable for a large variety of analytes and has proven particularly useful for analyte antibodies of the IgG and IgM class present in samples due to infections by pathogens. Also disclosed is a reagent kit useful for the method comprising at least two analyte-specific binding partners one of which carries a detectable label and a label-specific binding partner that binds to said detectable label but itself does not carry a detectable label.

公开(公告)号：US20180128825A1

公开(公告)日：2018-05-10

申请号：US15621280

申请日：2017-06-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara Upmeier ,  Toralf Zarnt ,  Johannes Polz

IPC: G01N33/543 ,  G01N33/68

CPC classification number: G01N33/54393 ,  G01N33/54306 ,  G01N33/6854 ,  G01N2800/26

Abstract: The present invention relates to a method for determining an analyte in a sample suspected to comprise said analyte, comprising a) contacting with said sample at least a first and a second capture compound for said analyte, wherein said first and second capture compounds are non-identical capture compounds, and wherein said capture compounds compete in binding to said analyte; b) contacting said capture compounds contacted with said sample with a specifier, wherein said specifier competes in binding to said capture compounds with said analyte; c) determining the amount of complexes comprising said specifier and a capture compound; and d) determining said analyte in a sample based on the result of step c). The present invention further relates to a method for improving the specificity of an indirect immunoassay for determining an analyte, comprising replacing at least 10% of a capture compound by a non-identical capture compound; wherein the capture compound replaced competes in binding to said analyte with the capture compound introduced. The present invention further relates to kits, devices, and uses related to the aforementioned methods.