公开(公告)号：US20190324024A1

公开(公告)日：2019-10-24

申请号：US16450176

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Erasmus Huber ,  Hans-Peter Josel ,  Thomas Meier ,  Michael Schraeml ,  Leopold Von Proff

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US20190309091A1

公开(公告)日：2019-10-10

申请号：US16450186

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Lars Hillringhaus ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Hans-Peter Josel ,  Christoph Seidel ,  Michael Schraeml ,  Leopold Von Proff

IPC: C07K16/44 ,  G01N33/543

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US11372000B2

公开(公告)日：2022-06-28

申请号：US16538886

申请日：2019-08-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Klaus Hallermayer ,  Monika Soukupova ,  Christina Porzig ,  Ulrike Kurtkaya ,  Leopold Von Proff ,  Sonja Fiesel ,  Marion Herrmann ,  Stefanie Kern

IPC: C07K16/26 ,  A61K39/395 ,  G01N33/68 ,  C07K14/575 ,  C07K14/58

Abstract: The present disclosure relates to an antibody that specifically binds a mutated NT-proBNP having i) a mutation substituting arginine at position 46 with histidine or ii) a mutation substituting glutamic acid at position 43 with aspartic acid. Moreover, the present disclosure relates to a mutated NT-proBNP or fragment thereof. Further, envisaged by the present disclosure are kits containing the antibody of the present disclosure, or the mutated NT-proBNP of the present disclosure. The present disclosure also concerns a method for diagnosing heart failure.