公开(公告)号：US12158466B2

公开(公告)日：2024-12-03

申请号：US16734884

申请日：2020-01-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Schraeml ,  Markus Roessler ,  Michael Gerg

IPC: G01N33/543 ,  G01N33/53

Abstract: The present invention relates to a method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule as well as kits, compositions and uses relating thereto.

公开(公告)号：US20230406909A1

公开(公告)日：2023-12-21

申请号：US18251393

申请日：2021-10-29

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Ute Jucknischke ,  Ulrike Kurtkaya ,  Thomas Mock ,  Michael Schraeml ,  Sandrine Carolina Stiegler

IPC: C07K16/10

CPC classification number: C07K16/1003 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2317/565 ,  C07K2317/567

Abstract: The present invention relates to monoclonal antibodies binding to the nucleocapsid protein of SARS-CoV-2 virus, nucleic acids encoding said antibody, host cells producing the same, compositions and kits comprising said antibodies, as well as methods of detecting SARS-CoV-2 virus in a sample comprising using said antibodies.

公开(公告)号：US10301383B2

公开(公告)日：2019-05-28

申请号：US15003988

申请日：2016-01-22

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Herbert Andres ,  Hartmut Duefel ,  Michael Gerg ,  Frank Kowalewsky ,  Christian Scholz ,  Michael Schraemi

IPC: C07K16/00 ,  C07K16/22 ,  C12N9/90 ,  C07K16/12 ,  G01N33/74 ,  C07K16/40 ,  G01N33/573 ,  C07K14/475 ,  C07K14/65

Abstract: Isolated antibodies that specifically bind to an epitope comprised in the stretch of amino acids ranging from amino acid 76 to amino acid 84 of human insulin-like growth factor-1 precursor (SEQ ID NO:1). Use of the novel antibodies for the sensitive and specific detection of insulin-like growth factor-1, in some embodiments while in the presence of high excess concentration of insulin-like growth factor-2, for example in a bodily fluid sample.

公开(公告)号：US20140120561A1

公开(公告)日：2014-05-01

申请号：US14069445

申请日：2013-11-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Herbert Andres ,  Hartmut Duefel ,  Michael Gerg ,  Frank Kowalewsky ,  Christian Scholz ,  Michael Schraeml

IPC: G01N33/74 ,  C07K16/22

CPC classification number: C07K16/22 ,  C07K14/475 ,  C07K14/65 ,  C07K16/1203 ,  C07K16/40 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/92 ,  C07K2319/00 ,  C07K2319/21 ,  C07K2319/22 ,  C07K2319/23 ,  C07K2319/24 ,  C07K2319/35 ,  C07K2319/41 ,  C07K2319/43 ,  C12N9/90 ,  C12Y502/01008 ,  G01N33/573 ,  G01N33/74 ,  G01N2333/99

Abstract: Isolated antibodies that specifically bind to an epitope comprised in the stretch of amino acids ranging from amino acid 76 to amino acid 84 of human insulin-like growth factor-1 precursor (SEQ ID NO:1). Use of the novel antibodies for the sensitive and specific detection of insulin-like growth factor-1, in some embodiments while in the presence of high excess concentration of insulin-like growth factor-2, for example in a bodily fluid sample.

Abstract translation: 分离的抗体特异性结合包含在人类胰岛素样生长因子-1前体的氨基酸76至氨基酸84的氨基酸区段中的表位（SEQ ID NO：1）。 在一些实施方案中，当存在高过量浓度的胰岛素样生长因子-2（例如在体液样品中）时，使用新型抗体用于敏感和特异性检测胰岛素样生长因子-1。

公开(公告)号：US20240400672A1

公开(公告)日：2024-12-05

申请号：US18662694

申请日：2024-05-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Hans-Peter Josel ,  Ute Jucknischke ,  Holger Lehmann ,  Anastasia Markina ,  Giuseppe Prencipe ,  Leopold von Proff ,  Michael Schraeml ,  Steinar Halldórsson

IPC: C07K16/26 ,  G01N33/78

Abstract: The present invention provides a novel monoclonal antibody specifically binding to L-Thyroxine (T4) and compositions and kits comprising such antibodies. Furthermore, provided are polynucleotides encoding such monoclonal antibodies, host cells expressing said antibodies, methods of producing such antibodies and diagnostic methods using such monoclonal antibodies. The monoclonal antibody of the invention comprises a heavy chain variable domain (VH) comprising V or A in position 33; Y in position 50; W in position 52; I in position 98, G, A or V in position 99; Y in position 100; and I in position 100b; and a light chain variable domain (VL) comprising amino acids H or Y in position 28; N or K in position 29; W in position 32; G or A in position 91; Y, W or F in position 92; S or T in position 93; Y or F in position 95b; N, S, T or Q in position 95c; and H in position 96, wherein the positions of the amino acids in the VH and the VL are indicated according to the Kabat numbering scheme, respectively.

公开(公告)号：US20230095167A1

公开(公告)日：2023-03-30

申请号：US17801469

申请日：2021-02-19

Applicant: Universiteit Maastricht ,  Academisch Ziekenhuis Maastricht ,  Roche Diagnostics Operations, Inc.

Inventor： Alfred Engel ,  Michael Gerg ,  Ute Jucknischke ,  Johann Karl ,  Peter Kastner ,  Thomas Meier ,  Lars Hillringhaus ,  Ulrich Schotten ,  Vinzent Rolny ,  Ursula-Henrike Wienhues-Thelen ,  André Ziegler ,  Roberto Latini ,  Jennifer Marie Theresia Anna Meessen

IPC: G01N33/74 ,  G01N33/68

Abstract: The present invention relates to a method for assessing atrial fibrillation in a subject, said method comprising the steps of determining the amount of BMP10 in a sample from the subject, and comparing the amount of BMP10 to a reference amount, whereby atrial fibrillation is to be assessed. Moreover, the present invention relates to a method for diagnosing heart failure based on the determination of BMP 10 in a sample from a subject. Further, the present invention relates to a method for predicting the risk of a subject of hospitalization due to heart failure based on the determination of a BMP10-type peptide in a sample from a subject. The present invention further pertains to antibodies which bind to one or more BMP10-type peptides such as NT-proBMP10.

公开(公告)号：US11466098B2

公开(公告)日：2022-10-11

申请号：US16740778

申请日：2020-01-13

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Bernhard Eckert ,  Michael Gerg ,  Johann Karl ,  Martin Kaufmann ,  Julia Riedlinger ,  Magdelena Swiatek-de Lange ,  Lars Hillringhaus ,  Klaus Hirzel ,  Marcus-Rene Lisy

IPC: C07K16/36 ,  G01N33/574 ,  G01N33/86

Abstract: The present disclosure relates to specific binding agents binding to different PIVKA-II forms as compared to antibodies known so far in the art. The present disclosure also relates to methods of using the specific binding agents to detect the presence of PIVKA-II.

公开(公告)号：US11415576B2

公开(公告)日：2022-08-16

申请号：US15614856

申请日：2017-06-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Elke Faatz ,  Michael Gerg ,  Hans-Peter Josel ,  Christian Vogl

IPC: G01N33/537 ,  C07K16/26 ,  G01N33/82 ,  G01N33/541

Abstract: The present invention concerns an in vitro method for measurement of 25-hydroxyvitamin D, wherein the potentially interfering compound 24,25-dihydroxyvitamin D3 is blocked by a binding agent specifically binding to 24,25-dihydroxyvitamin D3 and not binding to 25-hydroxyvitamin D.

公开(公告)号：US20190324024A1

公开(公告)日：2019-10-24

申请号：US16450176

申请日：2019-06-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Klaus Hirzel ,  Caroline Dorothea Hojer ,  Erasmus Huber ,  Hans-Peter Josel ,  Thomas Meier ,  Michael Schraeml ,  Leopold Von Proff

IPC: G01N33/543 ,  C07K16/44

Abstract: The present invention relates to a monoclonal antibody capable of binding to biotin. In one embodiment the monoclonal antibody according to the invention also does not bind to a biotin moiety on a biotinylated molecule, wherein the biotin moiety is attached to the molecule via the carbon atom of the carboxyl function of the valeric acid moiety of biotin. Also disclosed is a method for generation of an antibody as disclosed herein. The monoclonal antibody according to the invention is of specific use in a method for measuring an analyte in a sample, wherein a (strept)avidin/biotin pair is used to bind a biotinylated analyte specific binding agent to a (strept)avidin coated solid phase.

公开(公告)号：US20160252500A1

公开(公告)日：2016-09-01

申请号：US15146226

申请日：2016-05-04

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Schraeml ,  Markus Roessler ,  Michael Gerg

IPC: G01N33/543

Abstract: The present invention relates to a method for determining the total amount and/or concentration of an analyte in the presence of a binding molecule as well as kits, compositions and uses relating thereto.

Abstract translation: 本发明涉及用于在结合分子存在下测定分析物的总量和/或浓度的方法以及与其相关的试剂盒，组合物和用途。