公开(公告)号：US20160264925A1

公开(公告)日：2016-09-15

申请号：US15161358

申请日：2016-05-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Christian Birkner ,  Herbert von der Eltz ,  Monika Soukopova

IPC: C12N1/06 ,  C12N1/20

CPC classification number: C12N1/06 ,  C12N1/20

Abstract: The invention provides a composition containing an aqueous liquid phase, bacterial cells, and an ionic compound dissolved in the liquid phase. The ionic compound is selected from the group consisting of 1-butyl-3-methyl-imidazolium-thiocyanate, 1-butyl-3-methyl-imidazolium-2(2-methoxy-ethoxy)ethylsulfate, 1-methyl-1-[4-(3-methyl-3H-imidazol-1-ium)-but-1-yl]-3H-imidazolium-di(toluylsulfate), and 1-butyl-3-methyl-imidazolium-octylsulfate. The compositions of the invention are advantageously used for preparing lysates of biological cells, particularly bacterial cells.

Abstract translation: 本发明提供一种含有水相液相，细菌细胞和溶于液相的离子化合物的组合物。 离子化合物选自1-丁基-3-甲基 - 咪唑鎓 - 硫氰酸酯，1-丁基-3-甲基 - 咪唑鎓-2（2-甲氧基 - 乙氧基）乙基硫酸酯，1-甲基-1- [4 - （3-甲基-3H-咪唑-1-基） - 丁-1-基] -3H-咪唑鎓 - 二（甲苯基硫酸盐）和1-丁基-3-甲基 - 咪唑鎓辛基硫酸盐。 本发明的组合物有利地用于制备生物细胞，特别是细菌细胞的裂解物。