公开(公告)号：US11099154B2

公开(公告)日：2021-08-24

申请号：US15366695

申请日：2016-12-01

Applicant: Roche Diagnostics Operations, Inc. ,  Owlstone Medical Limited

Inventor： Uwe Kobold ,  Roland Thiele ,  Noah Weiss ,  Lauren Brown

IPC: G01N27/624 ,  G01N33/82 ,  G01N33/487 ,  H01J49/00

Abstract: A method of quantifying the amount of at least two analytes A1 and A2 involving (a) adding at least one salt (S) to at least a portion (P1) of the sample comprising the at least two analytes A1 and A2, (b) ionizing at least a portion of the sample according to (a) thereby forming an analyte flow comprising the analytes A1 and A2 in ionized form, (c) separating the ionized analytes A1 and A2 from each other by using at least one ion mobility separator (124), wherein the analyte flow according to (b) at least partially passes through the ion mobility separator (124), and (d) quantifying the amount of the separated ionized analytes obtained according to (c), wherein A1 is a pharmaceutically active compound C or derivative thereof and A2 is a metabolite or stereoisomer of C.

公开(公告)号：US20170082578A1

公开(公告)日：2017-03-23

申请号：US15366695

申请日：2016-12-01

Applicant: Roche Diagnostics Operations, Inc. ,  Owlstone Medical Limited

Inventor： Uwe Kobold ,  Roland Thiele ,  Noah Weiss ,  Lauren Brown

IPC: G01N27/62 ,  H01J49/00 ,  G01N33/487

CPC classification number: G01N27/624 ,  G01N33/487 ,  G01N33/82 ,  H01J49/0031

Abstract: A method of quantifying the amount of at least two analytes A1 and A2 involving (a) adding at least one salt (S) to at least a portion (P1) of the sample comprising the at least two analytes A1 and A2, (b) ionizing at least a portion of the sample according to (a) thereby forming an analyte flow comprising the analytes A1 and A2 in ionized form, (c) separating the ionized analytes A1 and A2 from each other by using at least one ion mobility separator (124), wherein the analyte flow according to (b) at least partially passes through the ion mobility separator (124), and (d) quantifying the amount of the separated ionized analytes obtained according to (c), wherein A1 is a pharmaceutically active compound C or derivative thereof and A2 is a metabolite or stereoisomer of C.

公开(公告)号：US11016098B2

公开(公告)日：2021-05-25

申请号：US16158931

申请日：2018-10-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Uwe Kobold ,  Roland Thiele ,  Noah Weiss ,  Benjamin Tiemann

IPC: G01N33/68 ,  H01J49/00 ,  G01N33/58 ,  G01N33/96 ,  B01D59/44 ,  G01N30/72 ,  G01N1/28

Abstract: A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.

公开(公告)号：US20190388402A1

公开(公告)日：2019-12-26

申请号：US16562629

申请日：2019-09-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Ewelina Hegel ,  Hans-Peter Josel ,  Eloisa Lopez-Calle ,  Josef Roedl ,  Noah Weiss ,  Benjamin Tiemann

IPC: A61K31/436 ,  A61K47/18 ,  A61K9/08

Abstract: The present description relates to a liquid composition having at least one immune-modulatory macrolide compound and an essentially cell-free blood hemolysate. The present description further relates to a method for manufacturing a stabilized immune-modulatory macrolide calibration solution, by a) preparing a solution comprising an essentially cell-free blood hemolysate, b) admixing a predetermined amount of at least one immune-modulatory macrolide into said solution comprising an essentially cell-free blood hemolysate, and, thereby c) manufacturing a stabilized immune-modulatory macrolide calibration solution. Furthermore, the present description relates to kits, uses, devices, methods and to an immune-modulatory macrolide compound calibration solution related thereto.

公开(公告)号：US20190049460A1

公开(公告)日：2019-02-14

申请号：US16158931

申请日：2018-10-12

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Uwe Kobold ,  Roland Thiele ,  Noah Weiss ,  Benjamin Tiemann

IPC: G01N33/68 ,  G01N33/58 ,  G01N30/72 ,  B01D59/44

Abstract: A method and an apparatus for determining a concentration of a target analyte in a sample of bodily fluid are disclosed. The method involves providing a sample of bodily fluid including the target analyte, providing an internal standard solution including a mixture of components having a plurality of isotopes of the target analyte, wherein a concentration of each isotope is unknown, adding the internal standard solution to the sample, analyzing the sample including the internal standard solution using a mass spectrometer, creating a sample function curve based on signal intensities, wherein the signal intensities define arbitrary units, transferring an analyte signal into a corresponding arbitrary analyte unit by means of the sample function curve, and transferring the arbitrary analyte unit into the concentration of a target analyte by means of a standardization function representing a curve of concentrations depending on the arbitrary units.