公开(公告)号：US20250042976A1

公开(公告)日：2025-02-06

申请号：US18704654

申请日：2022-10-25

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Gerg ,  Simon Jochum ,  Ute Jucknischke ,  Ulrike Kurtkaya ,  Michael Schraeml ,  Sandrine Carolina Stiegler

IPC: C07K16/10

Abstract: The present invention relates to monoclonal antibodies binding to the Receptor Binding Domain of the Spike protein of SARS-CoV-2 virus, nucleic acids encoding said antibody, host cells producing the same, compositions and kits comprising said antibodies, method of detecting SARS-CoV-2 virus in a sample comprising using said antibodies and methods of using said antibodies in immunoassays.

公开(公告)号：US20240059746A1

公开(公告)日：2024-02-22

申请号：US18506022

申请日：2023-11-09

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Juliane Benz ,  Simon Jochum ,  Matthias Hemann

IPC: C07K14/435 ,  C07K14/005 ,  G01N33/569 ,  G01N33/58

CPC classification number: C07K14/435 ,  C07K14/005 ,  G01N33/56988 ,  G01N33/58 ,  G01N33/581 ,  C07K2319/40 ,  G01N2333/16 ,  G01N2469/20

Abstract: Fusion polypeptides are disclosed which are substrates for Kutzneria albida transglutaminase. The fusion polypeptides comprise one or more FKBP chaperone(s) and a target polypeptide. Each of these elements is separated from the neighboring element by a linker amino acid sequence. It was found that inserting glutamic acid containing transglutaminase recognition motifs into the linker amino acid chains is advantageous. Subsequent labeling reactions catalyzed by the transglutaminase surprisingly provide labeled fusion polypeptides have superior properties when compared with chemically random-labeled fusion polypeptides of similar design. Assays and kits are provided for in vitro detection of target antibodies in samples.